Project description:shRNAs targetting p65, NFIA, or GFP (control) were expressed in High Passage Growth Arrested BJ fibroblasts maintained in rapamycin, which were then maintained or withdrawn from rapamycin and harvested for RNA-seq
Project description:BJ fibroblasts were grown in the presence of rapamycin until reaching growth arrest at high passages, at which point cells were maintained in or withdrawn from rapamycin. RNA was then harvested for RNA-seq
Project description:BJ fibroblasts were grown in the presence of rapamycin until reaching growth arrest at high passages, at which point cells were maintained in or withdrawn from rapamycin, and RNA was then harvested for RNA-seq
Project description:BJ fibroblasts were grown in the presence of rapamycin until reaching growth arrest at high passages, at which point cells were maintained in or withdrawn from rapamycin, while also being treated with control or SMAD2/3 regulating ligands. RNA was then harvested for RNA-seq
Project description:BJ Fibroblasts were maintained in rapamycin and were passaged until reaching growth arrest at very high passages. These cells were then maintained in or withdrawn from rapamycin, during which time they were given either control treatments or the p65 inhibitor TPCA-1.
Project description:BJ Fibroblasts were maintained in rapamycin and were passaged until reaching growth arrest at very high passages. These cells were then maintained in or withdrawn from rapamycin, during which time they were given either control treatments or treatments with factors regulating SMAD2/3 activity.
Project description:Genome wide expression analysis of high passage growth arrested BJ fibroblasts maintained or withdrawn from rapamycin maintenance, upon control or transcription factor knockdown
Project description:Genome wide expression analysis of high passage growth arrested BJ fibroblasts maintained or withdrawn from rapamycin maintenance, in the presence or absence of various SMAD2/3 regulating molecules
Project description:Genome wide analysis of the effect of p65 inhibition upon the epigenomic landscape in high passage growth arrested cells being withdrawn from rapamycin