Altered lncRNAs transcriptomic profiles in atherosclerosis-induced ischemic stroke
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ABSTRACT:
PROVIDER: PRJNA612294 | ENA |
REPOSITORIES: ENA
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Project description:Long non-coding RNAs (lncRNAs) can not only regulate gene transcription and translation, but also participate in the development of central nervous system diseases as epigenetic modification factors. However, their functional significance in atherosclerosis-induced ischemic stroke (AIIS) is unclear. The study aimed to screen out differentially expressed lncRNAs (delncRNAs), and to elucidate their potential regulatory mechanisms in the pathophysiology of AIIS. We screened out 10 patients with AIIS and recruited 10 healthy volunteers. We used microarray to detect the whole blood RNA of subjects, and explored the biological functions of delncRNAs by GO and KEGG analysis. After further analyzing the delncRNAs of THP-1 stimulated by ox-LDL, selective lncRNAs were screened. Through co-expression analysis, a corresponding lncRNA-mRNA interaction network was constructed. We yielded 180 delncRNAs (44 up-regulated and 136 down-regulated) and 218 demRNAs (45 up-regulated and 173 down-regulated). Lnc-SCARNA8 and lnc-SNRPN-2 are the highest elevated and decreased lncRNA in AIIS, respectively. The delncRNAs may play a significant role in ubiquitination-mediated protein degradation signaling pathways. In THP-1 cells models, the expression levels of lnc-SLC22A16-3, lnc-MTRNR2L1-10 and lnc-ACAT1-4 were consistent to the microarray results. According to lncRNA-mRNA network, the expression of vacuolar protein sorting 13 homolog B (VPS13B) and biliverdin reductase B (BLVRB) were significantly regulated. This study identified selective delncRNAs through microarray detection and validated by in vitro cell experiment. Our findings suggest that the ubiquitinated proteasome pathway, VPS13B and BLVRB may play a fundamental role in the pathological process of AIIS.
2020-03-13 | GSE146882 | GEO
Project description:Purpose: This study aimed to explore the differential expression profiles of exosomal lncRNAs and evaluated their potential utility in the accurate diagnosis of LAA stroke. Methods: LncRNA profiles of exosomes in large artery atherosclerosis stroke and controls were generated by high-throughput sequencing. The sequence reads that passed quality filters were analyzed at the transcript isoform level with Hisat2, Trapnell, STAR. qRT–PCR validation was performed using TaqMan and SYBR Green assays. Results: A total of 1020 differentially expressed lncRNAs were identified in LAA stroke patients. GO and KEGG pathway analyses indicated that their target genes are involved in atherosclerosis-related pathways, including inflammation, cell adhesion, and cell migration. 8 exosomal lncRNAs were confirmed with qRT–PCR.The result showed that the expression trend of differential expressed lncRNAs in validation was consistent with RNA-seq.Conclusion: Our study showed the differential expression of lncRNAs in plasma exosomes and presented related diagnostic potential for LAA stroke for the first time. The results suggested that exosomal lncRNA could be potential diagnostic tools in LAA stroke. Circular RNAs (circRNAs), novel endogenous noncoding RNAs, play diverse roles in ischemic stroke. Recently, the abundance and stability of circRNAs in exosomes have been identified. However, a comprehensive analysis of exosomal circRNAs in large artery atherosclerotic (LAA) stroke has not yet been reported. We performed RNA sequencing (RNA-Seq) to comprehensively identify differentially expressed exosomal circRNAs in five paired LAA and normal controls. RNA-Seq identified a total of 462 circRNAs in peripheral exosomes; there were 25 differentially expressed circRNAs among them. Additionally, circRNA competing endogenous RNA (ceRNA) network and translatable analysis revealed the potential functions of the exosomal circRNAs in LAA progression. Two ceRNA pathways involving 5 circRNAs, 2 miRNAs, and 3 mRNAs were confirmed by qRT-PCR. In the validation cohort, receiver operating characteristic (ROC) curve analysis identified two circRNAs as possible novel biomarkers, and a logistic model combining two and four circRNAs increased the area under the curve compared with the individual circRNAs.
2021-06-08 | GSE173719 | GEO
Project description:We performed a genome-wide methylation study in whole-blood DNA from 404 ischemic stroke patient cohort, distributed across 3 ischemic stroke subtypes: Large-artery atherosclerosis (n=132), Small-artery disease (n=141) and Cardio embolic (n=127) . Illumina HumanMethylation450 BeadChip array was used to measure DNA methylation in CpG sites. We performed a genome-wide methylation study in whole-blood DNA from 185 ischemic stroke patient cohort. Illumina HumanMethylation450 BeadChip array was used to measure DNA methylation in CpG sites.
2015-08-19 | GSE69138 | GEO
Project description:Residual risk of stroke recurrence persists despite the administration of the prevention measures. The recurrent risk is particularly high in patients with atherosclerosis. However, the underlying mechanisms leading to atherosclerosis progression and recurrent stroke have not been fully understood. We found that stroke accelerated atherosclerosis progression.FTO overexpression suppressed atheroprogression after stroke via inhibiting autophagy-dependent ferroptosis.
2026-07-01 | GSE307081 | GEO
Project description:Residual risk of stroke recurrence persists despite the administration of the prevention measures. The recurrent risk is particularly high in patients with atherosclerosis. However, the underlying mechanisms leading to atherosclerosis progression and recurrent stroke have not been fully understood. We found that stroke accelerated atherosclerosis progression.FTO overexpression suppressed atheroprogression after stroke via inhibiting autophagy-dependent ferroptosis.
2026-07-01 | GSE307034 | GEO
Project description:Residual risk of stroke recurrence persists despite the administration of the prevention measures. The recurrent risk is particularly high in patients with atherosclerosis. However, the underlying mechanisms leading to atherosclerosis progression and recurrent stroke have not been fully understood. We found that stroke accelerated atherosclerosis progression.FTO overexpression suppressed atheroprogression after stroke via inhibiting autophagy-dependent ferroptosis.
2026-07-01 | GSE307033 | GEO
Project description:Residual risk of stroke recurrence persists despite the administration of the prevention measures. The recurrent risk is particularly high in patients with atherosclerosis. However, the underlying mechanisms leading to atherosclerosis progression and recurrent stroke have not been fully understood. We found that stroke accelerated atherosclerosis progression.
2026-07-01 | GSE307032 | GEO
Project description:Residual risk of stroke recurrence persists despite the administration of the prevention measures. The recurrent risk is particularly high in patients with atherosclerosis. However, the underlying mechanisms leading to atherosclerosis progression and recurrent stroke have not been fully understood. We found that stroke accelerated atherosclerosis progression.
2026-07-01 | GSE308369 | GEO
Project description:Our study reveals that ischemic stroke can influence the expression of LncRNAs and mRNAs in the peripheral blood at both the acute and subacute stages
2019-02-27 | GSE122709 | GEO
Project description:Circulating angiogenic profiles in ischemic stroke and intracranial atherosclerosis (ICAD)
| PRJNA1380502 | ENA