Project description:The purpose of this study is to determine whether cross feeding relationships can contribute to the alleviation of colitis. The two strains used in this study are Anaerostipes caccae and Bifidobacterium bifidum. Both strains are clearly present in significant amounts in the intestines of healthy humans. A. caccae is a butyrate-producing bacterium that uses lactic acid and acetate to produce butyrate. On the other hand, B. bifidum is known to produce lactic acid and acetate. The purpose of this study is to confirm the effect of the ecological network between the two strains on the improvement of colitis, particularly in terms of protecting intestinal epithelial cells.
Project description:Transcriptional analysis upon the overexpression of the folate gene cluster on a high copy plasmid when compared to an empty vector in the lactic acid bacterium L. plantarum. The transcriptional response was determined for both strains in continuous culture in the presence of glucose thereby using the hybridisation sceme as described in the data processing section. Keywords: comparative genomic hybridization Transcriptional analysis upon the overexpression of the folate gene cluster on a high copy plasmid when compared to an empty vector in the lactic acid bacterium L. plantarum. The transcriptional response was determined for both strains in continuous culture in the presence of glucose thereby using the hybridisation sceme as described in the data processing section.
