Project description:Large White and Meishan pigs were either non-treated or injected with mammalian 1-24 ACTH (Immediate Synachten, Novartis France) at the dose of 250 µg per animal. Pigs were sacrificed either immediately after capture from their home cage (non-treated animals) or 1 hour following ACTH injection. Adrenal glands were immediately collected from pigs and frozen on dry ice and then stored at -80°C until RNA isolation. Keywords: stress response, adrenal, gene expression, pig
Project description:Large White and Meishan pigs were either non-treated or injected with mammalian 1-24 ACTH (Immediate Synachten, Novartis France) at the dose of 250 µg per animal. Pigs were sacrificed either immediately after capture from their home cage (non-treated animals) or 1 hour following ACTH injection. Adrenal glands were immediately collected from pigs and frozen on dry ice and then stored at -80°C until RNA isolation. Keywords: stress response, adrenal, gene expression, pig 47 samples
Project description:In this study, we applied scRNA-Seq to profile the PBMCs from Meishan and Duroc pigs which are two strains with distinctive immune traits, and applied scATAC-Seq to Duroc PBMCs. We expect this study will improve the understanding about the immune heterogeneity and inter-breed immune variations in pigs, and reveal novel immune cell populations, immune genes and regulators that would facilitate future biomedical studies. MS(Meishan pig); DR(Duroc pig).
