Project description:The mechanistic target of rapamycin complex 1 (mTORC1) regulates beta cell growth and mass; yet it remains unclear whether it also directs beta cell functional maturation. To understand the global molecular basis of the phenotype caused by the loss of Raptor in beta cells, we isolated pancreatic islets from 8-week-old βRapKO and WT mice. We compared gene-expression profile by Affymetrix microarray of islets, which revealed that a number of mRNAs were dys-regulated in Raptor-deficient islets.
Project description:To understand the global molecular basis of the phenotype caused by the loss of Zzef1 in islets, we isolated pancreatic islets from 12-week-old Zzef1KO and WT mice fed with normal-chow diet (NCD). Then mice were sacrificed and total RNAs were isoloated from islets. We included at least 3-4 independent biological replicates, each composed of RNA isolated from islets of 5 animals with the same genotype. We then performed gene expression profiling analysis using data obtained from islets RNA-seq of Zzef1KO mice and littermates fed a normal chow diet.
Project description:To investigate a role of UCP2 in pancreatic β-cells, we performed whole genome microarray in islets from βUCP2Tg mice, which overexpressed UCP2 in specifically β-cells.
Project description:Npc1 is a cholesterol transporter located on lysosomal membrane, and whether it is involved in regulating the function and differentiation of β cells is unclear. To understand molecular basis of the phenotype caused by Npc1 loss in β cells, we isolated islets from 2-week-old WT and Npc1KO mice for single-cell sequencing. We compared the gene expression profiles of different subpopulation of islet cells and found many changes of islet subpopulation and biological pathway occurred in Npc1KO islets.
Project description:The pancreas islets RNA expressions were compared between WT (exon2 floxed) mice and Cdh13 KO (exon2 deleted) mice. The islets were isolated from the mice at 8wks old, 14wks old under normal chow diet, and 14wks old under HFD conditions.
Project description:To obtain comprehensive profile of gene expression following a pancreatectomy, we performed microarray analysis of genes expressed in islets of C57BL/6J mice 3 days after 60% pancreatectomy or sham operation. gene expression in mouse pancreatic islets was measured at 3 days after 60% pancreatectomy or sham operation.
Project description:To obtain comprehensive profile of gene expression following a pancreatectomy, we performed microarray analysis of genes expressed in islets of C57BL/6J mice 3 days after 60% pancreatectomy or sham operation.