Dataset Information

ABSTRACT: BS-Amplicon Rot HEK293

PROVIDER: PRJNA615220 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Dataset's files

Source:

			Action	DRS
	SRR11425020_1.fastq.gz	Fastqsanger.gz
	SRR11425020_2.fastq.gz	Fastqsanger.gz
	SRR11425021_1.fastq.gz	Fastqsanger.gz
	SRR11425021_2.fastq.gz	Fastqsanger.gz
	SRR11425022_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 8

Similar Datasets

Project description:<p>Wheat is a major staple crop grown across the globe. Fusarium crown rot (FCR) of wheat, caused by Fusarium pseudograminearum, is a destructive soil-borne disease that lacks effective sustainable control measures. Here, we assembled a cross-kingdom synthetic microbial community (SMC) comprising Trichoderma harzianum T19, Bacillus subtilis BS-Z15, and four other Bacillus strains, and evaluated its biocontrol efficacy against FCR under non-sterile soil conditions. The SMC treatment significantly suppressed FCR, reducing the disease severity index by approximately 70%. Wheat growth and yield were simultaneously enhanced: SMC inoculation nearly doubled plant biomass (with fresh and dry weights ~100% higher) and increased thousand-kernel weight by ~14% compared to the controls. In the rhizosphere, SMC improved soil health by elevating soil organic matter and nitrogen levels by over 50%, while mitigating pathogen-induced nutrient imbalances (excess available P and K) and boosting nutrient-cycling enzyme activities. Amplicon sequencing revealed that SMC suppressed pathogenic Fusarium in the rhizosphere and enriched beneficial microbes, including antagonistic fungi (Trichoderma, Chaetomium) and plant growth-promoting bacteria (Pseudomonas, Paenibacillus). Co-occurrence network analysis showed that SMC treatment restructured the rhizosphere microbial network with higher connectivity, stability, and a prevalence of positive cooperative interactions under F. pseudograminearum stress. Defense-related metabolites, such as epi-jasmonic acid, allantoin, Nβ-acetyltryptamine, and dihydrodaidzein, accumulated to higher levels with SMC, consistent with KEGG enrichment in pathways related to amino acid biosynthesis, carbon metabolism, signal transduction, and plant defense. These findings demonstrate that the cross-kingdom SMC modulates soil nutrients, microbial community structure, and rhizosphere metabolites to synergistically promote wheat growth and enhance resistance to FCR.</p>

Dataset Information

Dataset's files

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets