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Expression data from Ulk1 silencing in mouse liver and AML12 cells

ABSTRACT: Expression data from Ulk1 silencing in mouse liver and AML12 cells

PROVIDER: PRJNA615632 | ENA |

REPOSITORIES: ENA

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Expression data from Ulk1 silencing in mouse liver and AML12 cells

Project description:This experiments detail the differential gene expressionin hepatic cells in response to Ulk1 silencing both in vivo and in vitro We used microarrays to detail the global programme of gene expression underlying the loss of ULK1 knockdownin hepatic cells

2020-03-27 | GSE147579 | GEO

Transcriptional regulation of genes in AML12 cells treated with Palmitic acid, LXR lingand (GW3965) and Ulk1 siRNA

Project description:Transcriptional regulation of genes in AML12 cells treated with Palmitic acid, LXR lingand (GW3965) and Ulk1 siRNA shows differential effect of Ulk1 KD on LXr responsive genes AML-12 cells co-treated with 0.75mM PA+/- 10ÂµM GW3965 for 24 h +/- Ulk1 SiRNA

2016-03-31 | E-GEOD-74059 | biostudies-arrayexpress

Transcriptional regulation of genes in AML12 cells treated with Palmitic acid, LXR lingand (GW3965) and Ulk1 siRNA

Project description:Transcriptional regulation of genes in AML12 cells treated with Palmitic acid, LXR lingand (GW3965) and Ulk1 siRNA shows differential effect of Ulk1 KD on LXr responsive genes

2016-03-31 | GSE74059 | GEO

Comprehensive Cytogenetic and Genomic Profiling of the Murine AML12 (Alpha Mouse Liver 12) Hepatocyte Cell Line

Project description:In our study, we aimed to provide a comprehensive cytogenetic and genomic profiling of the murine AML12 hepatocyte cell line. We established an integrated reference map that combines cytogenetics, short tandem repeat (STR) profiling, and transcriptomic analysis through RNA sequencing. Our findings reveal that AML12 maintains hepatocyte lineage identity while exhibiting partial de-differentiation and a complex karyotypic landscape characterized by a near-tetraploid chromosome set with recurrent structural rearrangements. This study serves as a valuable resource for the scientific community, enhancing the understanding of AML12's genetic characteristics and its implications for future research in liver biology and related fields.

2026-06-15 | GSE319199 | GEO

Irisin regulated genes in mouse liver derived AML12 cells

Project description:Irisin regulated genes in mouse liver derived AML12 cells

| PRJNA971191 | ENA

Irisin regulated genes in mouse liver derived AML12 cells

Project description:To find genes regulated by irisin in culture media in AML12 cells.

2023-09-25 | GSE232188 | GEO

DNA methylation data from AML12 cells during EMT

Project description:We investigated genome-wide DNA methylation during EMT in AML12 cells using comprehensive high throughput arrays for relative methylation (CHARM). We used custom Nimblegen microarrays. We isolated genomic DNA from cells at different timepoints post-TGF-β stimulation and hybridized to custom-designed Nimblegen microarrays (CHARM arrays). AML12 cells from 4 representative experiments were collected and used for the genome-wide DNA methylation analysis.

2011-07-03 | E-GEOD-27968 | biostudies-arrayexpress

Profiling of microRNAs in extracellular vesicles derived from mouse normal hepatocyte line AML12

Project description:Previously we have shown that AML12 cell-derived extracellular vesicles (EVs) exhibit anti-fibrotic effects in human hepatic stellate cells in vitro and during toxin-induced liver injury in mice. The mechanisms by which AML12 EVs ameliorate liver fibrosis have not been fully investigated and microRNAs are among the EV payload components that are delivered to recepient cells to regulate their functionsl properties. In this study, EVs were isolated by differential centrifugation of culture supernatants from AML12 cells that ahd been maintained in serum-free conditions. The EVs were characterized by Nanosight Tracking Analysis and Western blot. Three different batches of AML12 EVs were used to isolate total RNAs by QIAGEN miRNeasy kit, and approximately 200 ng of RNAs were subjected to small RNA-seq. A total of 224 miRNAs were identified, with miR-122-5p, miR-378a-3p, miR-210-3p, miR-320-3p, and miR-125a-5p ranked as the top five most abundant miRNAs.

2026-06-30 | GSE327951 | GEO

Effect of DUSP6 overexpression or knockdown on gene expression in AML12 cells

Project description:DUSP6 plays important roles in MAPK signaling pathway, but whether and how it is involved in liver funciton remains to be explored. Here, we performed RNA-seq analyses in AML12 cells where DUSP6 is disrupted. We overexpressed GFP-DUSP6 or GFP in AML12 cells, and tested the effects of DUSP6 increase on gene expression in AML12 cells. Meanwhile, we knocked down DUSP6 in AML12 cells and tested the effects of DUSP6 decrease on gene expression in AML12 cells. Taken together, we analyzed the changes of gene expression mediated by DUSP6, which provides new insights for the function of DUSP6 in liver

2023-12-31 | GSE249146 | GEO

Central Role of ULK1 in Type I Interferon Signaling

Project description:We provide evidence that the Unc-51-like kinase 1 (ULK1) is phosphorylated and activated during engagement of the Type I IFN receptor. Our studies demonstrate that the function of ULK1 controls expression of key interferon stimulated genes (ISGs) that mediate important biological functions, including anti-viral and antineoplastic responses. Total RNA was isolated from untreated and IFNβ-treated Ulk1/2+/+ and Ulk1/2-/- mouse embryonic fibroblasts (MEFs). Cells were treated for 6 hours with or without mouse IFNβ (2500 IU/ml).

2014-08-26 | E-GEOD-60778 | biostudies-arrayexpress

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