L. lactis cultures: control vs acetaldehyde-induced
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ABSTRACT:
PROVIDER: PRJNA616125 | ENA |
REPOSITORIES: ENA
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Project description:Transcriptome analysis of Lactococcus lactis cultures comparing control non-induced and induced with acetaldehyde
2020-03-30 | GSE147696 | GEO
Project description:L. lactis cultures: control vs diacetyl-induced
| PRJNA616126 | ENA
Project description:Transcriptome analysis of Lactococcus lactis cultures comparing control non-induced and induced with diacetyl
2020-03-30 | GSE147695 | GEO
Project description:MCF12A monolayer cultures were exposed to ethanol or acetaldehyde for eiher 1 week or 4 weeks Total RNA samples were assayed for gene expression and for microRNA expression. MicroRNA expression was accomplished using 2 experiments. The first experiment included untreated control, ethanol treated (4 weeks at 2.5 mM) and acetaldehyde treated (4 weeks at 1 mM). The data are presented in order of control1 for the ethanol and acetaldehyde treated samples followed by the ethanol and acetaldehyde treated samples, and then the control2 for the soft agar selected sample followed by the soft agar selected sample. The microRNA names are included in separate columns.
2017-02-10 | GSE76952 | GEO
Project description:Alcoholism is associated with breast cancer incidence and progression, and moderate chronic consumption of ethanol is a risk factor. The mechanisms involved in alcohol's oncogenic effects are unknown, but it has been speculated that they may be mediated by acetaldehyde. Here, we use the immortalized normal human epithelial breast cell line MCF-12A to determine whether short- or long-term exposure to ethanol or to acetaldehyde, using in vivo compatible ethanol concentrations, induces their oncogenic transformation and/or the acquisition of epithelial mesenchymal transition (EMT). Cultures of MCF-12A cells were incubated with 25 mM ethanol or 2.5 mM acetaldehyde for 1 week, or with lower concentrations (1.0-2.5 mM for ethanol, 1.0 mM for acetaldehyde) for 4 weeks. In the 4 wk incubation, cells were also tested for anchorage independence, including isolation of soft agar selected cells (SASC) from the 2.5 mM ethanol incubations. Cells were analyzed by immuno-cytofluorescence, flow cytometry, western blotting, DNA microarrays, RT/PCR, and assays for miRs. We found that short-term exposure to ethanol, but not, in general, to acetaldehyde, was associated with transcriptional upregulation of the metallothionein family genes, alcohol metabolism genes, and genes suggesting the initiation of EMT, but without related phenotypic changes. Long-term exposure to the lower concentrations of ethanol or acetaldehyde induced frank EMT changes in the monolayer cultures and in SASC as demonstrated by changes in cellular phenotype and mRNA expression. This suggests that low concentrations of ethanol, with little or no mediation by acetaldehyde, induce EMT and some traits of oncogenic transformation such as anchorage independence in normal breast epithelial cells. MCF12A monolayer cultures were exposed to ethanol or acetaldehyde for either 1 week or 4 weeks. Total RNA samples were assayed for gene expression and for microRNA expression.
2017-02-10 | GSE76951 | GEO
Project description:Alcoholism is associated with breast cancer incidence and progression, and moderate chronic consumption of ethanol is a risk factor. The mechanisms involved in alcohol's oncogenic effects are unknown, but it has been speculated that they may be mediated by acetaldehyde. Here, we use the immortalized normal human epithelial breast cell line MCF-12A to determine whether short- or long-term exposure to ethanol or to acetaldehyde, using in vivo compatible ethanol concentrations, induces their oncogenic transformation and/or the acquisition of epithelial mesenchymal transition (EMT). Cultures of MCF-12A cells were incubated with 25 mM ethanol or 2.5 mM acetaldehyde for 1 week, or with lower concentrations (1.0-2.5 mM for ethanol, 1.0 mM for acetaldehyde) for 4 weeks. In the 4 wk incubation, cells were also tested for anchorage independence, including isolation of soft agar selected cells (SASC) from the 2.5 mM ethanol incubations. Cells were analyzed by immuno-cytofluorescence, flow cytometry, western blotting, DNA microarrays, RT/PCR, and assays for miRs. We found that short-term exposure to ethanol, but not, in general, to acetaldehyde, was associated with transcriptional upregulation of the metallothionein family genes, alcohol metabolism genes, and genes suggesting the initiation of EMT, but without related phenotypic changes. Long-term exposure to the lower concentrations of ethanol or acetaldehyde induced frank EMT changes in the monolayer cultures and in SASC as demonstrated by changes in cellular phenotype and mRNA expression. This suggests that low concentrations of ethanol, with little or no mediation by acetaldehyde, induce EMT and some traits of oncogenic transformation such as anchorage independence in normal breast epithelial cells. MCF12A monolayer cultures were exposed to ethanol or acetaldehyde for either 1 week or 4 weeks. Total RNA samples were assayed for gene expression and for microRNA expression.
2017-02-10 | GSE76950 | GEO
Project description:Mycobacterium tuberculosis: yidC (Rv3921c) induced Vs Control cultures
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Project description:Our transcriptome data shows that the two-component system CesSR, which senses cell envelope stresses of different origins, is one of the major players when L. lactis is forced to overproduce the endogenous membrane protein BcaP, a branched-chain amino acid permease. Two-condition experiment: nisin induced overproduction of BcaP vs. control. Biological replicates: 2 controls, 2 overproduction cultures; independently grown and harvested; dye swaped on the second array.
2011-07-19 | E-GEOD-30776 | biostudies-arrayexpress
Project description:<p>Lactobacillus has antioxidant properties that may benefit poultry production. However, there is no systematic research on antioxidant of Lactobacillus strain and its effects on regulating nutrient metabolism in aged laying hens. This study investigated the influence of Leuconostoc lactis on production and antioxidant capacity in aged laying hens and explored the key biomarkers associated with tryptophan-skatole metabolism and its effects on the intestinal microbiota-liver axis. Hens supplemented with L. lactis showed a higher laying rate, reduced hepatic MDA levels, and increased T-AOC in comparison with the control group (CG). Indole-3-acetaldehyde (IAld) levels were elevated in both feces and yolk, and skatole decreased in feces by the L. lactis group compared to CG. The total polyunsaturated fatty acids (PUFAs), C18:3n3, and C18:2n6c in yolk were raised in the L. lactis group relative to CG. In the liver, mRNA levels of AhR, CYP2D6, and CPT-1 were markedly upregulated in the L. lactis group relative to CG. The L. lactis-treated group also exhibited higher alpha diversity in fecal samples at 30 days and in ileal samples at 60 days. Further, we conducted the hepatocyte validation experiment and found that MDA levels were significantly reduced, and T-AOC was increased in both the L. lactis and IAld-treated groups compared with the CG. IAld treatment significantly affected p38, and NF-κB, and Nrf2 cytokine expression in hepatocytes. The findings provide a reference for the use of L. lactis in improving production and intestinal nutrition in aged laying hens.</p>
2025-09-09 | MTBLS12964 | MetaboLights
Project description:Gene expression analysis of MCF12A human breast cultures exposed to ethanol or acetaldehyde
| PRJNA309120 | ENA