Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Next Generation Sequencing Facilitates Quantitative Analysis of Transcriptome profile in IEC 4.1-WT and IEC4.1-Ifnar1-/- KO cells (C12 KO) in response to Cryptosporidium parvum

ABSTRACT: Next Generation Sequencing Facilitates Quantitative Analysis of Transcriptome profile in IEC 4.1-WT and IEC4.1-Ifnar1-/- KO cells (C12 KO) in response to Cryptosporidium parvum

PROVIDER: PRJNA616254 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR11448400.fastq.gz	Fastqsanger.gz
	SRR11448401.fastq.gz	Fastqsanger.gz
	SRR11448402.fastq.gz	Fastqsanger.gz
	SRR11448403.fastq.gz	Fastqsanger.gz
	SRR11448404.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 12

Similar Datasets

Next Generation Sequencing Facilitates Quantitative Analysis of Transcriptome profile in IEC 4.1-WT and IEC4.1-Ifnar1-/- KO cells (C12 KO) in response to Cryptosporidium parvum

Project description:This study aims to measure the impact of Ifnar1-/- knockdown (KO) on the transcriptome profile in cultured IEC 4.1 cell in response to C. parvum infection. IEC4.1-WT cells and IEC4.1-Ifnar1-/- cells (stably CRISPR/Cas9 knockout; C12 KO cells) were exposed to C. parvum infection. Cells treated with a scrambled non-specific siRNA were used as the control. Total RNA was collected for the genome-wide analysis.

2021-10-20 | GSE147720 | GEO

ISG induction in ∆7bp IFNAR2 mutant, IFNAR1 KO and wild type THP1.

Project description:ISG induction in IFNAR2 mutant, IFNAR1 KO and wild type THP1

2023-01-25 | GSE211502 | GEO

Transcriptome profile of intestinal epithelium in conditional Ifnar1 ko and wt mice following Cryptosporidium parvum Infection

Project description:This study aims to measure the transcriptome profile of ilium tissues from intestinal epithelial conditional Ifnar1 KO and control littermates following cryptosporidial infection. Neonates of the conditional intestinal epithelial Ifnar1 knockout mice and control littlermates were exposed to C. parvum infection for 48 and 72h. Ilium tissues were collected and total RNA was isolated for the genome-wide analysis.

2023-01-04 | GSE164279 | GEO

Dependency of ISG expression on IFNAR1 and Tyk2

Project description:We analyze the expression profile of ISGs in the context of IFNAR1-KO primary murine B cells and macrophages. These analses were used to define ISG gene sets that are under tonic control. Furthermore, these analyses enabled the definition of ISGs that are dependent on Tyk2 signaling. Male C57BL/6 IFNAR1-KO mice were injected subcutaneously with 10,000U IFNa for 2hrs. Splenic B cells and peritoneal macrophages were sorted by FACS directly into TriZol and gene expression profiling was performed on Affymetrix Mouse Gene 1.0 ST Arrays.

2015-11-20 | E-GEOD-75203 | biostudies-arrayexpress

Transcriptome Profile of IEC4.1 cells deficient with Prdm1 in response to IFN-gamma

Project description:This study is to investigate the potential role of Prdm1 in murine intestinal epithelial cells (IEC4.1 cells) in response to IFN gamma stimulation. Prdm1 was knocked down in IEC4.1 cells by using a CRISPR/Cas9 approach. Wild type IEC4.1 cells were used as control (Ctrl). Cells were treated with or without mouse IFN gamma (1ng/ml for 4hrs). Then total RNA was collected for sequencing via DNBSEQ platform to obtain a comprehensive view of the transcriptome.

2025-05-30 | GSE245345 | GEO

Investigation of the direct activity of type I interferon on Ifnar1 KO ID8 ovarian cancer cells in vitro.

Project description:This experiment is part of a larger study examining the anti-tumour properties of interferon epsilon in ovarian cancer. The goal of this experiment was to assess whether interferon signalling had been ablated in Ifnar1 CRISPR knockout (KO) ID8 cell lines, so that they could be used for in vivo models of ovarian cancer.

2023-03-28 | GSE215261 | GEO

Next Generation Sequencing Facilitates Quantitative Analysis of Transcriptome profile in IEC 4.1 cells in response to Cryptosporidium parvum

Project description:The lincRNA-NR_033736 is an early-primary gene controlled by the type I IFN signaling in murine intestinal epithelial cells. This study aims to measure the impact of lincRNA-NR_033736 knockdown on the transcriptome profile in cultured IEC 4.1 in response to C. parvum infection. Cells were treated with a specific siRNA to knockdown lincRNA-NR_033736 and then exposed to C. parvum infection. Cells treated with a scrambled non-specific siRNA were used as the control. Total RNA was collected for the genome-wide analysis using RNA-seq

2021-01-12 | GSE145464 | GEO

Next-generation sequencing facilitates quantitative analysis of transcriptome profile in IEC 4.1 cells in response to CSpV1 RNA transfection

Project description:The Cryptosporidium parvum virus 1 (CSpV1) is a member of the family Partitiviridae, genus Cryspovirus that infects C. parvum. This study aims to measure the impact of CSpV1 on the transcriptome profile in cultured IEC 4.1. Cells were treated with specific dsRNAs encoding CSpV1 viral capsid protein (CA) and RNA dependent RNA polymerase (RdRp). Cells treated with a scrambled non-specific siRNA were used as the control. Total RNA was collected for the genome-wide analysis, for sequencing using BGISEQ-500 platform.

2023-01-05 | GSE164316 | GEO

Transcriptome analysis of IEC4.1 cells in response to TNFα stimulation.

Project description:Mouse intestinal epithelial cells (IEC4.1 cells) were stimulated with TNFα (10 ng/ml) for 4h. The Agilent SurePrint G3 Mouse Gene Expression Microarray (G4852A) was used for the analysis, which provides full coverage of genes and transcripts with the most up-to-date content, including mRNAs and lincRNAs (http://www.chem.agilent.com/store/en_US/Prod-G4852A/G4852A). IEC4.1 cells were grown to 80% confluence for four groups: the siRNA control (Group A, cells treated with a non-specific scrambied siRNA control), the TNFα -stimulated (Group B, cells treated with the siRNA control plus TNFα stimulation), lincRNA-Cox2 siRNA (Group C, cells treated with an siRNA to lincRNA-Cox2), and lincRNA-Cox2 siRNA/ TNFα stimulated (Group D, cells treated with the lincNRA-Cox2 siRNA plus TNFα stimulation). Cells were treated with the siRNAs for 24h, followed by additional culture for 4h in the presence or absence of TNFα (10 ng/ml). Total RNAs were prepared with the RNeasy Mini kit (Qiagen) according to the manufacturer’s instruction (Ambion).

2016-10-25 | E-MTAB-3904 | biostudies-arrayexpress

ISG induction in ∆7bp IFNAR2 mutant, IFNAR1 KO and wild type THP1.

Project description:ISG induction in ∆7bp IFNAR2 mutant, IFNAR1 KO and wild type THP1.

| PRJNA870508 | ENA