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Single-cell transcriptomic resolution of human pancreatic islets reveals cellular states and intercellular interactions associated with type 1 diabetes

ABSTRACT: Single-cell transcriptomic resolution of human pancreatic islets reveals cellular states and intercellular interactions associated with type 1 diabetes

PROVIDER: PRJNA623002 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR11477627.fastq.gz	Fastqsanger.gz
	SRR11477628.fastq.gz	Fastqsanger.gz
	SRR11477629.fastq.gz	Fastqsanger.gz
	SRR11477630.fastq.gz	Fastqsanger.gz
	SRR11477631.fastq.gz	Fastqsanger.gz

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Single-cell transcriptomic resolution of human pancreatic islets reveals cellular states and intercellular interactions associated with type 1 diabetes

Project description:Our transcriptomic phenotyping of pancreatic cell types provides novel insights into pancreas biology, as well as the initial pathogenic events in T1D.

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Epigenetic changes in Langerhans islets preceding the onset of diabetes

Project description:Diabetes-resistant and diabetes-prone female New Zealand Obese mice were classified based on liver fat content and early blood glucose concentrations at 10 weeks of age before the onset of T2D. By using transcriptome and DNA methylome analysis of Langerhans islets, we identified early epigenetic alteration in mice and humans which could serve as putative epigenetic biomarkers

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N6-methyladenosine (m6A) profiling of type II diabetes islets

Project description:In type 2 diabetes, pancreatic beta-cells fail to compensate for the presence of insulin resistance in target tissues and represent a central player in the disease development. Identifying and studying innovative molecular mechanisms that lead to beta-cell failure in diabetes represent an interesting line of research and are necessary. N6-Methyladenosine (m6A) is the most abundant modification in mRNA and is found virtually in all mammals. Through m6A-profiling, we aim to characterize the dynamic RNA methylation changes in islets obtained from patients with type 2 diabetes.

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Human Pluripotent Stem Cell-derived Islets Ameliorate Diabetes in Nonhuman Primates [human_singlecell]

Project description:Pluripotent stem cell-derived islets (hPSC-islets) are a promising cell resource for diabetes treatment. Here, we demonstrate that transplantation of pluripotent stem cell-derived islets into diabetic nonprimates effectively restored endogenous insulin secretion and improved glycemic control. Single-cell RNA sequencing analysis of S6D2 clusters confirmed the existence of the three major pancreatic endocrine cell populations (β cells, α-like cells and δ-like cells) and their proportions, which altogether accounted for 80%. Importantly, hierarchical clustering of S6D2 hCiPSC-islets, 10 wpt kidney grafts and primary islets showed that the hCiPSC differentiated pancreatic endocrine cells shared similar global gene expression profiles to their native counterparts in primary islets. Single-cell RNA sequencing analysis on PBMCs revealed the potential immune response of recipient macaque to hCiPSC-islets.

2022-02-04 | GSE185036 | GEO

Human Pluripotent Stem Cell-derived Islets Ameliorate Diabetes in Nonhuman Primates [monkey_singlecell]

Project description:Human pluripotent stem cell-derived islets (hPSC-islets) are a promising cell resource for diabetes treatment. Here, we demonstrate that transplantation of human pluripotent stem cell-derived islets into diabetic nonhuman primates effectively restored endogenous insulin secretion and improved glycemic control. Single-cell RNA sequencing analysis of S6D2 clusters confirmed the existence of the three major pancreatic endocrine cell populations (β cells, α-like cells and δ-like cells) and their proportions, which altogether accounted for 80%. Importantly, hierarchical clustering of S6D2 hCiPSC-islets, 10 wpt kidney grafts and primary human islets showed that the hCiPSC differentiated pancreatic endocrine cells shared similar global gene expression profiles to their native counterparts in primary human islets. Single-cell RNA sequencing analysis on PBMCs revealed the potential immune response of recipient macaque to hCiPSC-islets.

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Human Pluripotent Stem Cell-derived Islets Ameliorate Diabetes in Nonhuman Primates [human_bulk]

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Single-cell RNA-seq uncovers the pathological alterations of pancreatic islets in type 2 diabetes mice

Project description:Pancreatic islet beta cell heterogeneity has been identified, which plays a pivotal role in the pathological alterations of pancreatic islets in type 2 diabetes (T2D) mice. However, pathological alterations of beta cells in type 2 diabetes (T2D) mice remain to be investigated. We isolated pancreatic islets from the control and T2D mice and conducted scRNA-seq analysis using the 10x Genomics platform. Pathological alterations of beta cells in T2D were also explored.

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N6-methyladenosine (m6A) profiling of type II diabetes islets

Project description:N6-methyladenosine (m6A) profiling of type II diabetes islets

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Enriched Alternative Splicing in Islets of Diabetes-Susceptible Mice

Project description:Enriched Alternative Splicing in Islets of Diabetes-Susceptible Mice

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DNA methylation profiling of male human pancreatic islets identifies loci for type 2 diabetes

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