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PAgo-induced DNA interference protects bacteria from invader DNA

ABSTRACT: pAgo-induced DNA interference protects bacteria from invader DNA

PROVIDER: PRJNA625099 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR11541039.fastq.gz	Fastqsanger.gz
	SRR11541040.fastq.gz	Fastqsanger.gz
	SRR11541041.fastq.gz	Fastqsanger.gz
	SRR11541042.fastq.gz	Fastqsanger.gz
	SRR11541043.fastq.gz	Fastqsanger.gz

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pAgo-induced DNA interference protects bacteria from invader DNA [smDNA]

Project description:pAgo-induced DNA interference protects bacteria from invader DNA [smDNA]

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pAgo-induced DNA interference protects bacteria from invader DNA [gDNA]

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pAgo-induced DNA interference protects bacteria from invader DNA [smDNA]

Project description:Members of the conserved Argonaute protein family use small RNA guides to find their mRNA targets to regulate gene expression and suppress mobile genetic elements in eukaryotes. Argonautes are also present in many bacterial and archaeal species. Unlike eukaryotic proteins, several studied prokaryotic Argonautes use small DNA guides to cleave DNA, a process dubbed DNA interference. However, the natural functions and targets of DNA interference are poorly understood and the mechanisms of DNA guide generation and target discrimination remain unknown. Here, we studied the in vivo activities of a bacterial Argonaute nuclease CbAgo and demonstrated that it induces cleavage of multicopy genetic elements, including plasmids, transposons and repetitive chromosomal loci. Generation of small DNA guides employed by CbAgo requires cooperation between its intrinsic endonuclease activity and the cellular double-strand break repair machinery. The mechanism of guide generation ensures that small DNA guides are enriched in sequences that target foreign DNA and endows CbAgo with capacity to eliminate plasmids and fight phage infection. Similar principles may underlie the specificity of self-nonself discrimination by diverse defense systems in prokaryotes.

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pAgo-induced DNA interference protects bacteria from invader DNA [gDNA]

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pAgo-induced DNA interference protects bacteria from invader DNA

Project description:This SuperSeries is composed of the SubSeries listed below.

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Cyanobacterial Argonautes and Cas4 family nucleases cooperate to interfere with invading DNA

Project description:Prokaryotic Argonaute proteins (pAgos) from the long-A clade are stand-alone immune systems that use small interfering (si)DNA guides to recognize and cleave invading plasmid and virus DNA. Certain long-A pAgos are co-encoded with accessory proteins with unknown functions. Here, we show that cyanobacterial long-A pAgos act in conjunction with Argonaute-associated Cas4 family enzyme 1 (ACE1). Structural and biochemical analyses reveal that ACE1-associated pAgos mediate siDNA-guided DNA interference, akin to stand-alone pAgos. ACE1 is structurally homologous to the nuclease domain of bacterial DNA repair complexes and acts as a single-stranded DNA endonuclease that processes siDNA guides. pAgo and ACE1 form a heterodimeric APACE1 complex, which modulates ACE1 activity. While ACE1-associated pAgos alone interfere with plasmids and bacteriophages, plasmid interference is boosted when pAgo and ACE1 are co-expressed. Our study reveals that pAgo-mediated immunity is enhanced by accessory proteins and broadens our mechanistic understanding of how pAgo systems interfere with invading DNA.

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