Project description:To know the role of C/EBPb in the changes of H3K27ac during desidualization, we compared the H3K27 profiles between cAMP-stimulation and cAMP-stimulation under C/EBPb-knockdown.
Project description:To know the role of C/EBPb in the change of mRNA expression during desidualization, we compared the mRNA expression profiles between cAMP-stimulation and cAMP-stimulation under C/EBPb-knockdown.
Project description:Timecourse of cAMP-induced decidualization of endometrial stromal cells.<br><br>Note that files GSM5962.txt and GSM5965.txt as imported from GEO are identical.
Project description:To know the differences of decidualization induced by different stimuli, we compared the mRNA expression profiles of decidualized ESCs stimulated by cAMP or cAMP+MPA for 4 days, or by MPA or E2+MPA for 14 days.
Project description:Endometrial stromal decidualization is a hormone-regulated process essential for establishing uterine receptivity and supporting embryo implantation. Three-dimensional (3D) endometrial organoid systems provide a physiologically relevant platform to study this process; however, how different stimulatory conditions influence decidualization responses in 3D culture remains incompletely characterized. In this study, we generated 3D human endometrial stromal organoids and subjected them to four experimental conditions: vehicle control, cyclic adenosine monophosphate (cAMP) stimulation, combined estrogen–progesterone–cAMP (EPC) treatment, and co-culture with in vitro–grown follicles derived from mouse ovary. Organoids were cultured for 6 days under each condition, followed by bulk RNA sequencing. Principal component analysis (PCA) revealed clear separation between control and stimulated groups, with cAMP, EPC, and follicle co-culture conditions showing distinct but partially overlapping transcriptional profiles. Differential gene expression analysis identified both shared and condition-specific transcriptional changes across the four treatments. Pathway-level analysis using gene set variation analysis (GSVA) further demonstrated common decidualization-associated biological processes alongside stimulus-specific functional responses. This dataset provides a comprehensive resource for investigating how distinct biochemical and paracrine stimuli regulate decidualization in 3D endometrial organoid systems. It enables comparative analysis of canonical cAMP-driven responses, hormone-mediated signaling, and follicle-derived paracrine effects on endometrial stromal decidualization.