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ABSTRACT: Transcriptomic insight into the pH variation and secondary metabolite production regulated by transcriptional factor PacC in Trichoderma harzianum

PROVIDER: PRJNA627508 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR11592054_1.fastq.gz	Fastqsanger.gz
	SRR11592054_2.fastq.gz	Fastqsanger.gz
	SRR11592055_1.fastq.gz	Fastqsanger.gz
	SRR11592055_2.fastq.gz	Fastqsanger.gz
	SRR11592056_1.fastq.gz	Fastqsanger.gz

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Expression data from tomato (Solanum lycopersicum) plants in interaction with different Trichoderma strains

Project description:Trichoderma harzianum T34 is a fungal strain able to promote the plant growth and to increase plant defense responses. Trichoderma harzianum transformants expressing the amdS gene, encoding an acetamidase, of Aspergillus nidulans produce a higher plant development than the wild type T34. We used microarrays to analyze the physiological and biochemical changes in tomato plants produced as consequence of interaction with Trichoderma harzianum T34 and amdS transformants

2015-12-25 | GSE76332 | GEO

Global transcriptome analysis of the pH regulator pacC in Colletotrichum gloeosporioides

Project description:Colletotrichum gloeosporioides, a widely distributed economically important agent in postharvest fruit disease thrives by massive secretion of ammonia to alkalize its environment and activate its pathogenicity genes. We sequenced the C. gloeosporioides transcriptomes of WT and a ∆pacC mutant, a major gene regulator under changing pH, under pH 7.0 in order to examine pacC regulation. Transcriptome analysis of the ∆pacC strain showed that 468 and 458 genes were up or down regulated, respectively. Both the up and down regulated genes were clustered into similar functions including: transporters to maintain cellular homeostasis, cell wall degrading enzymes to optimize pathogenicity and GATA-like transcription factors. Suggesting that activation of pacC under alkaline condition regulates genes expression to fit their optimal PKa. The transcript level predictions were verified by growth in different pH and fruit infection. Further analyses showed pacC binding sites were over-represented in the promoters of the pacC up-regulated genes but not in the promoters of the down-regulated genes, where instead, GATA-like binding sites were prominent. Evolutionary conservation of pacC control was sought by examining the gene orthologs in 5 fungal genomes whose members display contrasting alkaline or acidifying pathogenicity strategies. The results showed that irrespective of pathogen colonization strategy, only orthologs of up-regulated genes showed over-representation of pacC binding sites. Significantly, the down regulated orthologs revealed cross-genome over-representation of GATA transcription factor binding sites. Thus, pacC is a phylogenetically conserved fungal mechanism exerting dual pH control for maintaining homeostasis and pathogenicity in changing environments.

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Trichoderma harzianum CECT 2413 expression in liquid basal medium or glucose-, chitin-, or tomato plant-containing media

Project description:Trichoderma harzianum CECT 2413 expression in liquid basal medium and in the presence of glucose, chitin or tomato plants.

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Global transcriptome analysis of the pH regulator pacC in Colletotrichum gloeosporioides

Project description:Colletotrichum gloeosporioides, a widely distributed economically important agent in postharvest fruit disease thrives by massive secretion of ammonia to alkalize its environment and activate its pathogenicity genes. We sequenced the C. gloeosporioides transcriptomes of WT and a M-bM-^HM-^FpacC mutant, a major gene regulator under changing pH, under pH 7.0 in order to examine pacC regulation. Transcriptome analysis of the M-bM-^HM-^FpacC strain showed that 468 and 458 genes were up or down regulated, respectively. Both the up and down regulated genes were clustered into similar functions including: transporters to maintain cellular homeostasis, cell wall degrading enzymes to optimize pathogenicity and GATA-like transcription factors. Suggesting that activation of pacC under alkaline condition regulates genes expression to fit their optimal PKa. The transcript level predictions were verified by growth in different pH and fruit infection. Further analyses showed pacC binding sites were over-represented in the promoters of the pacC up-regulated genes but not in the promoters of the down-regulated genes, where instead, GATA-like binding sites were prominent. Evolutionary conservation of pacC control was sought by examining the gene orthologs in 5 fungal genomes whose members display contrasting alkaline or acidifying pathogenicity strategies. The results showed that irrespective of pathogen colonization strategy, only orthologs of up-regulated genes showed over-representation of pacC binding sites. Significantly, the down regulated orthologs revealed cross-genome over-representation of GATA transcription factor binding sites. Thus, pacC is a phylogenetically conserved fungal mechanism exerting dual pH control for maintaining homeostasis and pathogenicity in changing environments. Examination of C. gloeosporioides WT and a M-bM-^HM-^FpacC mutant grown under pH 7.0

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2010-10-13 | E-GEOD-24630 | biostudies-arrayexpress

Transcriptome analysis uncovers PacC-mediated metabolic adaptation in the dermatophyte pathogen Tricophyton interdigitale

Project description:Purpose: We evaluated T. interdigitale H6 and PacC-1 mutant transcriptome using high-throughput RNA-sequencing (RNA-seq) technology aiming to identify metabolic pathways modulated by the transcription factor PacC. Methods: We employed the next-generation sequencing (RNA-seq) approach to identify the central genes related to PacC in response to keratin. Results: PacC acts as a central regulator of fungal adaptation and virulence in keratin-rich conditions, coordinating nutrient uptake, keratin degradation, and oxidative stress responses. Its mutation causes severe metabolic imbalance and cellular stress. Conclusions: Our findings provide insights into a specific molecular function of PacC in a infection-like medium, highlighting PacC-dependent pH adaptation pathways as potential therapeutic targets in dermatophytoses.

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