Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

ABSTRACT: m6A sequencing of mice brain tissue

PROVIDER: PRJNA627805 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR11602473_1.fastq.gz	Fastqsanger.gz
	SRR11602473_2.fastq.gz	Fastqsanger.gz
	SRR11602474_1.fastq.gz	Fastqsanger.gz
	SRR11602474_2.fastq.gz	Fastqsanger.gz
	SRR11602475_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 10

Similar Datasets

m6A mapping in mouse RNA (mouse liver and human brain)

Project description:We developed a novel approach, m6A-seq, for high-resolution mapping of the transcriptome-wide m6A landscape, based on antibody-mediated capture followed by massively parallel sequencing Identification of m6A modified sequences in mouse liver and human brain

2012-04-25 | E-GEOD-37004 | biostudies-arrayexpress

m6A profiling of early, adolescent, aged and Alzheimer brains

Project description:m6A-seq was performed on 4 brain regions (cortex, cerebellum, hypothalmus and hippocampus) of 2 week, 4 week, 6 week, 26 week and 52 week old BL6 mice. m6A profiling was also performed on human adolescent and old brain tissue (region BA9). m6A-seq was also performed on WT and 5xFAD mice (Alzheimer).

2020-11-20 | GSE144032 | GEO

Single-cell m6A profiling in the mouse brain uncovers cell type-specific methylomes and age-dependent differential methylation [m6A]

Project description:N6-methyladenosine (m6A) is an abundant mRNA modification in the brain which plays important roles in neurodevelopment and brain function. However, due to technical limitations, global profiling of m6A sites within the individual cell types that make up the brain has not been possible. Here, we develop a mouse model that enables transcriptome-wide m6A detection in any tissue of interest and at single-cell resolution. We use these mice to map m6A across different brain regions and within single cells of the mouse cortex and discover a high degree of shared methylation across brain regions and cell types. However, we also identify a small number of differentially methylated mRNAs in neurons that encode important regulators of neuronal signaling, and we discover that microglia have lower levels of m6A compared to other cell types. Finally, we perform single-cell m6A mapping in aged mice and identify many transcripts with age-dependent changes in m6A.

2024-09-24 | GSE237871 | GEO

Single-cell m6A profiling in the mouse brain uncovers cell type-specific RNA methylomes and age-dependent differential methylation [aged study]

2024-09-24 | GSE268159 | GEO

Single-cell m6A profiling in the mouse brain uncovers cell type-specific RNA methylomes and age-dependent differential methylation [GLORI]

2024-09-24 | GSE267627 | GEO

Single-cell m6A profiling in the mouse brain uncovers cell type-specific RNA methylomes and age-dependent differential methylation [neuronuclei]

2024-09-24 | GSE267234 | GEO

Single-cell m6A profiling in the mouse brain uncovers cell type-specific RNA methylomes and age-dependent differential methylation

2024-09-24 | GSE266876 | GEO

Effect of acupuncture on gene expression in cerebral tissue of mice with cerebral hemorrhage

Project description:The present study aimed to identify and analyze the m6A profiles in the brain tissue of mice with intracerebral hemorrhage after acupuncture treatment.

2023-01-25 | GSE222968 | GEO

Profiling of mRNA N6-Adenosine Methylation (m6A) in Arabidopsis

Project description:m6A profiling in two accessions of Arabidopsis thaliana (Can-0 and Hen-16) using the m6A-targeted antibody coupled with high-throughput sequencing m6A-seq in two accessions of Arabidopsis, two replicates for each sample

2014-11-06 | E-GEOD-59154 | biostudies-arrayexpress

The tRNA methyltransferase dTrmt10A impacts m6A levels and the stress response in the Drosophila brain

Project description:The tRNA methyltransferase TRMT10A was previously shown modulating the level of N6-methyladenosine (m6A) in polyadenylated RNA (polyA+ RNA). Here, we focus on the function of the Drosophila homolog of human TRMT10A, CG14618/dTrmt10A, and its impact on m6A regulation in the Drosophila brain and acute stress response. Using m6A-IP RNA sequencing from head tissue, we find that altering the levels of dTrmt10A modulates the levels of m6A on polyA+ RNA in the brain. Specifically, dTrmt10A affects m6A levels on transcripts enriched for signaling pathways, with a particular impact on 3' UTR m6A in response to heat shock. Furthermore, our findings indicate that upregulation of dTrmt10A results in a global decrease in m6A levels and decreases resilience to acute heat stress. Collectively, our data affirm the crucial role of m6A in the biological response of the brain to stress, and reveal that dTrmt10A acts as an additional regulator of m6A in the Drosophila brain and stress response.

2023-12-12 | GSE229430 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data