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ABSTRACT: Cyp51A sequencing of Aspergillus fumigatus

PROVIDER: PRJNA629059 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR11637856.fastq.gz	Fastqsanger.gz
	SRR11637857.fastq.gz	Fastqsanger.gz
	SRR11637858.fastq.gz	Fastqsanger.gz
	SRR11637859.fastq.gz	Fastqsanger.gz
	SRR11637860.fastq.gz	Fastqsanger.gz

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Project description:Aspergillus fumigatus cyp51A sequence

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RNA-seq analysis of AtrR-regulated gene expression in Aspergillus fumigatus cells in response to voriconazole.

Project description:Aspergillus fumigatus transcription factor AtrR is a critical determinant of the azole resistance phenotype of this organism. AtrR positively regulates expression of a range of genes involved in azole resistance including the ATP-binding cassette transporter-encoding locus abcG1 (cdr1B/abcC) as well as the gene that encodes the enzymatic target of azole drugs cyp51A. Homology searches of A. fumigatus AtrR against a range of fungal species identified highly conserved 25 amino acid region in the carboxy-terminus. To determine the contribution of this region to AtrR function, we prepared a mutant from of the atrR that lacked this region (Δ855-879). We compared the response of an isogenic wild-type strain to a strain expressing the Δ855-879 AtrR derivative using RNA-seq, both in the absence and the presence of voriconazole challenge. The resulting data indicate that this 25 amino acid segment of AtrR is required for expression of some but not all AtrR target genes.

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Targeted sequencing of Cerberus T-cells (Cb-STs) and pentavalent specific T-cells (Penta-STs) targeting CMV, EBV, BKV, adenovirus and Aspergillus fumigatus

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Project description:RNA sequencing data from human monocyte-derived macrophages after infection with Aspergillus fumigatus.

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Comparative genomic hybridization of Aspergillus fumigatus Af71, Aspergillus fumigatus Af294, Aspergillus clavatus, Neosartorya fenneliae and Neosartorya fischeri with Aspergillus fumigatus Af293

Project description:Genomic DNA from five strains, Aspergillus fumigatus Af71, Aspergillus fumigatus Af294, Aspergillus clavatus, Neosartorya fenneliae, and Neosartorya fischeri, were co-hybridized with that of Aspergillus fumigatus Af293 and compared.

2005-05-15 | E-MEXP-333 | biostudies-arrayexpress

Project description:Cyp51A OF A. fumigatus

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Response of A549 cells treated with Aspergillus fumigatus

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Project description:Purpose: The goal of this study is to investigate the responses of HUVECs after the stimulation of conidia of A. fumigatus Methods: HUVECs were stimulated with conidia of Aspergillus fumigatus for 2 and 6 hours. Three biological repeats of stimulated cells or un-stimulated controls were send for RNA sequencing. Results: Using an optimized data analysis workflow, we mapped about 40 million sequence reads per sample to the human genome (build hg38) and identified round 80,000 transcripts in the HUVECs upon stimulation. Conclusions: Our resutls showed the detailed analysis of HUVECs transcriptomes upton conidia of Aspergillus fumigatus stimulation.

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Project description:cyp51A sequence of A. fumigatus

| PRJNA626476 | ENA