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Adaptive evolution provides a high-light stress tolerance in Synechocystis sp. PCC 6803

ABSTRACT: Adaptive evolution provides a high-light stress tolerance in Synechocystis sp. PCC 6803

PROVIDER: PRJNA630442 | ENA |

REPOSITORIES: ENA

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Adaptive evolution provides a high-light stress tolerance in Synechocystis sp. PCC 6803

Project description:we obtained a HL tolerant (Tol) strain Synechocystis sp. PCC 6803 by adaptive evolution experiment that the cells were repeatedly subcultured for prolonged periods of time (52 days) under high light stress condition (7000 to 9000 μmol m-2 s-1). Although the growth of the parental strain almost stopped under 9000 μmol m-2 s-1, no growth inhibition was observed in the Tol strain. Furthermore, the growth rate was identical to that of parental strain under low light condition (40 μmol m-2 s-1). To further investigate the high light tolerant mechanisms in the Tol strain, the transcriptome was performed. The transcriptome data suggests the increase of isiA expression in the Tol strain under HL condition. The overexpression of isiA successfully enhanced the HL stress tolerance in the parental strain. The HL tolerant mechanism was different from previous reported mechanisms, such as a reduction of the light-harvesting antenna size. The tolerant strain would be an attractive host for bio-production under high light conditions.

2020-05-06 | GSE149892 | GEO

Synechocystis sp. PCC 6803 trophic condition

Project description:Targeted proteome analysis of Synechocystis sp. PCC 6803 under photoautotrophic and mixotrophic conditions

2020-06-02 | PXD010010 | JPOST Repository

Transcriptome of Synechocystis sp. PCC 6803 strains with high light tolerance

Project description:Several strains with increased high light tolerance were previously generated by adaptive laboratory evolution from Synechocystis sp. PCC 6803 WT strain. The high light tolerance in each strain was caused by a few non-synonymous point mutations. Reintroduction of the corresponding point mutation in WT conferred enhanced tolerance to high light. Here, we characterized the responses at the transcriptional level using RNA-Seq approach to identify genes associated with the HL tolerance.

2025-08-01 | GSE268898 | GEO

Transcriptome analysis of the microalgae Synechocystis sp. PCC 6803 and mechanisms of photoinhibition tolerance under extreme high light conditions

Project description:To investigate acclimation mechanisms employed under extreme high light conditions, gene expression analysis was performed using the model microalgae Synechocystis sp. PCC 6803 (PCC 6803) cultured under various light intensities. From the low to the mid light conditions, the expression of genes related to light harvesting systems was repressed, whereas that of CO2 fixation and of D1 protein turnover-related genes was induced. Gene expression data also revealed that the down-regulation of genes related to flagellum synthesis (pilA2), pyridine nucleotide transhydrogenase (pntA and pntB), and sigma factor (sigA and sigF) represents acclimation mechanisms of PCC 6803 under excessive high light conditions.

2018-03-06 | GSE111408 | GEO

Synechocystis sp. PCC 6803

Project description:Synechocystis sp PCC 6803 Project

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Synechocystis sp. PCC 6803

Project description:Synechocystis sp. PCC 6803 Genome sequencing

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Synechocystis sp. PCC 6803

Project description:m5C_IP_Fastq of Synechocystis sp. PCC 6803

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