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Comparative analysis using different multiplicity of infection ratios from Chlamydia-infected epithelial cells

ABSTRACT: Comparative analysis using different multiplicity of infection ratios from Chlamydia-infected epithelial cells

PROVIDER: PRJNA630978 | ENA |

REPOSITORIES: ENA

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			Action	DRS
		Other
	SRR11728300.fastq.gz	Fastqsanger.gz
	SRR11728300_1.fastq.gz	Fastqsanger.gz
	SRR11728300_2.fastq.gz	Fastqsanger.gz
	SRR11728301.fastq.gz	Fastqsanger.gz

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Comparative analysis using different multiplicity of infection ratios from Chlamydia-infected epithelial cells

Project description:Here we examined gene expression from an in-vitro dual-RNA-seq experiment using Chlamydia trachomatis-infected HEp-2 epithelial cells. The experimental design consisted of three different multiplicity of infection ratio's (0.1, 1 and 10) across two different timeframes (1 and 24hrs), in addition to applying two different depletion methods (rRNA and rRNA + PolyA depletion). The aim was to examine and outline which MOI and depletion methods are most suited for bacterial-based infections.

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