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Gene expression profile of OT-I CD8+ T cell subtypes

ABSTRACT: Gene expression profile of OT-I CD8+ T cell subtypes

PROVIDER: PRJNA631424 | ENA |

REPOSITORIES: ENA

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Expression data from OT-I CD8 T cells activated with DC in the presence or absence of CpG-Induced Inflammation

Project description:Inflammatory cytokines promote the accumulation of activated CD8 T cells. Here, we transfer 600 OT-I CD8 T cells iv into naÃ¯ve C57BL/6 hosts. One day later, 500,000 LPS-matured and OVA257 peptide-coated DC were injected iv into OT-I CD8 T cell seeded hosts with (DC+CpG) or without (DC). Other seeded mice were infected with 2x10^4 virulent Listeria monocytogenes (vLM-OVA) iv. OT-I CD8 T cells were harvested from the spleen, flow sort purified, then RNA was extracted using RNeasy (Qiagen) kit. Naive OT-I CD8 T cells (Naive) were purified from the spleens of OT-I transgenic mice. Each group had three independent biological replicates.Transcriptomes were compared using DAVID analysis (with genes scoring FDR<0.01) and GSEA analysis. 3 biological replicates per group. Groups included NaÃ¯ve OT-I CD8 T cells, DC+CpG OT-I CD8 T cells, DC OT-I CD8 T cells, and vLM-OVA OT-I CD8 T cells. Most comparisons used NaÃ¯ve OT-I CD8 T cells as a baseline comparison

2013-12-10 | E-GEOD-53200 | biostudies-arrayexpress

Proteome profile of Traf6-OE OT-I T cells

Project description:Proteome profile of Traf6-OE OT-I T cells and EV OT-I cells after viral transfection

2025-05-06 | PXD039327 | Pride

Gene expression profile of CD8+ T cell subtypes

Project description:OT-I naïve T cells, central memory T cells, effector memory T cells and skin infiltrating T cells were sorted out from mice at different timepoints post infection OT-I cells from 15-20 mice were pooled together for each microarray dataset

2017-02-21 | GSE79805 | GEO

Comparison of gene expression of OT-I CD8 T cells after infection

Project description:The goal was to examine gene expression after in vivo activation by different vaccine strains of Listeria monocytogenes. Heat killed LM, irradiated LM and live actA- LM were used to immunize mice after transfer of OT-I cells 5x10e5 OT-I-RAG-/- cells transferred to B6 mice, 24hrs later mice immunized; 24hrs later cells sorted on MHC class II-, CD45.1+, CD8+, CD69+ Two arrays were performed per sample for a total of 8 arrays. RAW data file (supplementary file on the Series record) contains the data for each array individually.

2010-06-08 | E-GEOD-20265 | biostudies-arrayexpress

Gene expression profile of OT-I CD8+ T cell subtypes

Project description:Modified Vaccinia Ankara (MVA) was recently approved as a Smallpox vaccine. Transmission of Variola is by respiratory droplets, and MVA delivered by skin scarification (ss) protected mice far more effectively against lethal respiratory challenge with VACV than any other route of delivery, and at much lower doses. Comparisons of ss with intradermal, subcutaneous or intramuscular routes showed that MVAOVA ss-generated T cells were both more abundant and transcriptionally distinct. MVAOVA ss produced greater numbers of lung Ova-specific CD8+ TRM and was superior in protecting mice against lethal VACVOVA respiratory challenge. Nearly as many lung TRM were generated with MVAOVA ss compared to direct pulmonary immunization with MVAOVA, and both routes vaccination protected mice against lethal pulmonary challenge with VACVOVA. Strikingly, MVAOVA ss-generated effector T cells exhibited overlapping gene transcriptional profiles to those generated via direct pulmonary immunization. Overall, our data suggest that heterologous MVA vectors delivered via ss are uniquely well-suited as vaccine vectors for respiratory pathogens like COVID-19. In addition, MVA delivered via ss could represent a more effective dose-sparing smallpox vaccine.

2020-05-10 | GSE150190 | GEO

Differential activation of OT-1 CD8+ T cells by CD40-activated B cells and dendritic cells

Project description:To determine if CD40-activated B or dendritic cells can induce a similar or different genetic profile in OT-1 CD8+ T cells in early time points following activation

2016-12-31 | GSE76196 | GEO

Expression data from OT-I CD8 T cells activated with DC in the presence or absence of CpG-Induced Inflammation

Project description:Inflammatory cytokines promote the accumulation of activated CD8 T cells. Here, we transfer 600 OT-I CD8 T cells iv into naïve C57BL/6 hosts. One day later, 500,000 LPS-matured and OVA257 peptide-coated DC were injected iv into OT-I CD8 T cell seeded hosts with (DC+CpG) or without (DC). Other seeded mice were infected with 2x10^4 virulent Listeria monocytogenes (vLM-OVA) iv. OT-I CD8 T cells were harvested from the spleen, flow sort purified, then RNA was extracted using RNeasy (Qiagen) kit. Naive OT-I CD8 T cells (Naive) were purified from the spleens of OT-I transgenic mice. Each group had three independent biological replicates.Transcriptomes were compared using DAVID analysis (with genes scoring FDR<0.01) and GSEA analysis.

2013-12-10 | GSE53200 | GEO

Gene expression profile magnetic beads sorted CD8+ T cells from naïve splenocytes and OVA257-264 peptide activated splenocytes from WT and Susd2-/- OT-I mice

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2022-08-28 | GSE212179 | GEO

Gene expression profile at single cell level of OT-I CD8+ T cells from B16-OVA melanoma tumors

Project description:Gene expression profile at single cell level of OT-I CD8+ T cells from B16-OVA melanoma tumors

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Single-cell RNA sequencing of OT-I CD8+ T cells after stimulation with different affinity ligands

Project description:Activation of CD8+ T cells depends exquisitely on the affinity of the T cell receptor (TCR) for a peptide MHC (pMHC) ligand complex. Here, we activated OT-I transgenic CD8+ T cells with pure peptide and examined early activation responses by single-cell RNA-sequencing. T cells were activated with the high affinity OT-I cognate peptide (N4=SIINFEKL) for 1, 3 or 6 hours, or with reduced affinity peptides (T4=SIITFEKL and G4=SIIGFEKL) or the non-binding peptide (NP68=ASNENMDAM) for 6 hours. Cells were then sorted into 96-well plates by FACS and RNA was sequenced following an adapted Smart-Seq2 protocol.

2018-06-25 | E-MTAB-6051 | biostudies-arrayexpress

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