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Transcriptional response of fibronectin fragment treatment in human articular chondrocytes

ABSTRACT: Transcriptional response of fibronectin fragment treatment in human articular chondrocytes

PROVIDER: PRJNA631993 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR11778500_1.fastq.gz	Fastqsanger.gz
	SRR11778500_2.fastq.gz	Fastqsanger.gz
	SRR11778501_1.fastq.gz	Fastqsanger.gz
	SRR11778501_2.fastq.gz	Fastqsanger.gz
	SRR11778502_1.fastq.gz	Fastqsanger.gz

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Transcriptional response of fibronectin fragment treatment in human articular chondrocytes

Project description:Objective: Fibronectin is a matrix protein that is fragmented during cartilage degradation in osteoarthritis (OA). Treatment of chondrocytes with fibronectin fragments (FN-f) has been used to model OA in vitro, but the system has not been fully characterized. This study sought to define the transcriptional response of chondrocytes to FN-f, and directly compare it to responses traditionally observed in OA. Design: Normal human femoral chondrocytes isolated from tissue donors were treated with either FN-f or PBS (control) for 3, 6, or 18 hours. RNA-seq libraries were compared between time-matched FN-f and control samples in order to identify changes in gene expression over time. Differentially expressed genes were compared to a published OA gene set and used for pathway, transcription factor motif, and kinome analysis. Results: FN-f treatment resulted in 3,914 differentially expressed genes over the time course. Genes that are up- or downregulated in OA were significantly up- (p < 0.00001) or downregulated (p < 0.0004) in response to FN-f. Early response genes were involved in proinflammatory pathways, whereas many late response genes were involved in ferroptosis. The promoters of upregulated genes were enriched for NF-?B, AP-1, and IRF motifs. Highly upregulated kinases included CAMK1G, IRAK2, and the uncharacterized kinase DYRK3, while growth factor receptors TGFBR2 and FGFR2 were downregulated. Conclusions: FN-f treatment of normal human articular chondrocytes recapitulated many key aspects of the OA chondrocyte phenotype. This in vitro model is promising for future OA studies, especially considering its compatibility with genomics and genome-editing techniques.

2020-06-18 | GSE150411 | GEO

RNA-seq data from mouse articular chondrocytes

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Genes regulated by TGF-beta in bovine articular chondrocytes

Project description:Bovine articular chondrocytes were grown in micromass culture and were either untreated or treated with 5 ng TGF-b1/ml for 8 hours to identify genes regulated by TGF-b. Six total samples were analyzed. Three biological replicates of untreated bovine articular chondrocytes grown in micromass culture and three biological replicates of bovine articular chondrocytes grown in micromass culture and treated with 5ng TGF-b1/ ml for 8 hours.

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Synovial Fibroblast Gene Expression in Response to Fibronectin Fragment

Project description:Synovial Fibroblast Gene Expression in Response to Fibronectin Fragment

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Synovial Fibroblast Gene Expression in Response to Fibronectin Fragment

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Comparison between articular chondrocytes and mesenchymal stromal cells for the production of articular chondrocyte implants

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