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Chromosomal Mcm2-7 distribution and the genome replication program in species from yeast to humans

ABSTRACT: Chromosomal Mcm2-7 distribution and the genome replication program in species from yeast to humans

PROVIDER: PRJNA633612 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR15613656_1.fastq.gz	Fastqsanger.gz
	SRR15613656_2.fastq.gz	Fastqsanger.gz
	SRR15613657_1.fastq.gz	Fastqsanger.gz
	SRR15613657_2.fastq.gz	Fastqsanger.gz
	SRR15613658_1.fastq.gz	Fastqsanger.gz

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Chromosomal Mcm2-7 distribution and the genome replication program in species from yeast to humans

Project description:The spatio-temporal program of genome replication across eukaryotes is thought to be driven both by the uneven loading of pre-replication complexes (pre-RCs) across the genome at the onset of S-phase, and by differences in the timing of activation of these complexes during S-phase. To determine the degree to which distribution of pre-RC loading alone could account for chromosomal replication patterns, we mapped the binding sites of the Mcm2-7 helicase complex (MCM) in budding yeast, fission yeast, mouse and humans. We observed identical individual MCM double-hexamer (DH) footprints across the species, but notable differences in their distribution. Nonetheless, most fluctuations in replication timing in all four organisms could be accounted for by differences in chromosomal MCM distribution. We conclude that, although certain genomic regions, most notably the inactive X-chromosome, are subject to post-licensing regulation, most differences in replication timing along the chromosome reflect uneven chromosomal distribution of stochastically firing pre-replication complexes.

2021-05-18 | GSE150800 | GEO

Chromosomal Mcm2-7 distribution and the genome replication program in species from yeast to humans.

Project description:The spatio-temporal program of genome replication across eukaryotes is thought to be driven both by the uneven loading of pre-replication complexes (pre-RCs) across the genome at the onset of S-phase, and by differences in the timing of activation of these complexes during S phase. To determine the degree to which distribution of pre-RC loading alone could account for chromosomal replication patterns, we mapped the binding sites of the Mcm2-7 helicase complex (MCM) in budding yeast, fission yeast, mouse and humans. We observed similar individual MCM double-hexamer (DH) footprints across the species, but notable differences in their distribution: Footprints in budding yeast were more sharply focused compared to the other three organisms, consistent with the relative sequence specificity of replication origins in S. cerevisiae. Nonetheless, with some clear exceptions, most notably the inactive X-chromosome, much of the fluctuation in replication timing along the chromosomes in all four organisms reflected uneven chromosomal distribution of pre-replication complexes.

| S-EPMC8443269 | biostudies-literature

Kc167 Mcm2-7 Localization in HU

Project description:Determination of the genome-wide distribution of the Mcm2-7 helicase by chromatin immunoprecipitation in the Drosophila Kc167 cell line at the beginning of S phase. Kc167 cells were arrested at the G1/S transition with hydroxyurea (HU). Goal was to evaluate changes in the genome-wide Mcm2-7 distribution throughout the cell cycle, and how this relates to the excess Mcm2-7 loaded onto chromatin in G1. ChIP-Chip of Mcm2-7 in HU compared to input genomic DNA. Biological Replicates: 2

2014-11-27 | E-GEOD-63680 | biostudies-arrayexpress

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Kc167 Mcm2-7 Localization in Late S Phase

Project description:Determination of the genome-wide distribution of the Mcm2-7 helicase by chromatin immunoprecipitation in the Drosophila Kc167 cell line at the end of S phase. Kc167 cells were synchronized by first arresting at the G1/S transition with hydroxyurea (HU), and then releasing from the HU arrest point for 6 hours. Goal was to evaluate changes in the genome-wide Mcm2-7 distribution throughout the cell cycle, and how this relates to the excess Mcm2-7 loaded onto chromatin in G1.

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MCM2-7-dependent cohesin loading during S phase promotes sister-chromatid cohesion

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Project description:Evaluation of ORC and Mcm2-7 Distribution Throughout the Drosophila Kc Cell Cycle

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ARID1A regulates condensin II distribution and chromosomal partition [HiC]

Project description:ARID1A regulates condensin II distribution and chromosomal partition [HiC]

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ChIP-Seq experiment of Mcm2-HA in wild type and H3K37R yeast cells

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