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Time course XBP1s ChIP-Seq in Xbp1 flx/flx (WT, wild-type) mice and AlbCre Xbp1 flx/flx (XBP1 LKO, liver-specific knockout) mice

ABSTRACT: Time course XBP1s ChIP-Seq in Xbp1 flx/flx (WT, wild-type) mice and AlbCre Xbp1 flx/flx (XBP1 LKO, liver-specific knockout) mice

PROVIDER: PRJNA633946 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR11815158.fastq.gz	Fastqsanger.gz
	SRR11815159.fastq.gz	Fastqsanger.gz
	SRR11815160.fastq.gz	Fastqsanger.gz
	SRR11815161.fastq.gz	Fastqsanger.gz
	SRR11815162.fastq.gz	Fastqsanger.gz

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Time course Total RNA-Seq and XBP1s ChIP-Seq in Xbp1 flx/flx (WT, wild-type) mice and AlbCre Xbp1 flx/flx (XBP1 LKO, liver-specific knockout) mice

Project description:Time course Total RNA-Seq and XBP1s ChIP-Seq in Xbp1 flx/flx (WT, wild-type) mice and AlbCre Xbp1 flx/flx (XBP1 LKO, liver-specific knockout) mice

| PRJNA633943 | ENA

Time course XBP1s ChIP-Seq in Xbp1 flx/flx (WT, wild-type) mice and AlbCre;Xbp1 flx/flx (XBP1 LKO, liver-specific knockout) mice

Project description:We reported the hepatic XBP1s cistrome (starting at CT0 and proceeding every four hours for three complete 12-hour cycles) in the liver of wild-type mice and XBP1 liver-specific knockout mice; we also chose liver samples at CT0 and CT8 (four hours before the dusk 12-hour rhythmic peak) for comprehensive XBP1s ChIP-Seq to interrogate the genomic binding profiles of hepatic XBP1s in vivo.

2020-05-24 | GSE150889 | GEO

Time course Total RNA-Seq in Xbp1 flx/flx (WT, wild-type) mice and AlbCre Xbp1 flx/flx (XBP1 LKO, liver-specific knockout) mice

Project description:Time course Total RNA-Seq in Xbp1 flx/flx (WT, wild-type) mice and AlbCre Xbp1 flx/flx (XBP1 LKO, liver-specific knockout) mice

| PRJNA633945 | ENA

Time course Total RNA-Seq in Xbp1 flx/flx (WT, wild-type) mice and AlbCre;Xbp1 flx/flx (XBP1 LKO, liver-specific knockout) mice

Project description:We reported the hepatic total gene expression (starting at CT0 and proceeding every two hours for four complete 12-hour cycles) in the liver of wild-type mice and XBP1 liver-specific knockout mice.

2020-05-24 | GSE150888 | GEO

Time course Total RNA-Seq and XBP1s ChIP-Seq in Xbp1 flx/flx (WT, wild-type) mice and AlbCre;Xbp1 flx/flx (XBP1 LKO, liver-specific knockout) mice

Project description:This SuperSeries is composed of the SubSeries listed below.

2020-05-24 | GSE150890 | GEO

Time series XBP1s ChIP-Seq in wild-type mice and XBP1 liver-specific knockout mice

Project description:We reported the liver XBP1s cistrome (taken at 4h interval for a total of 48 hrs) in the liver of wild-type mice and XBP1 liver-specific knockout mice

2019-11-26 | GSE130760 | GEO

Profiling gene expression in 32Dcl3 cells following Xbp1 retrovirus vector transfection

Project description:The significant changes of hematopoietic cells induced by Xbp1S expression indicate that there is global alteration in gene expression. UPR induces transcription of Xbp1, and phosphorylation of the ER transmembrane kinase IRE1 initiates UPR-mediated mRNA splicing of Xbp1, resulting in the production of Xbp1S, an active form of a basic leucine zipper transcription factor. In the present study, Xbp1S retrovirus vector infected 32cl3 cells show cell cycle arrest and myeloid differentiation. Xbp1S may modulate important genes of differentiation and the cell cycle. RNA samples extracted from 32Dcl3 cells with pMIG (empty vector), pMY-Xbp1U (unspliced form) and pMY-Xbp1S (spliced form) retroviral vector transfected cells were subjected to expression profiling using the Affymetrix GeneChip microarray system.

2010-10-01 | E-GEOD-17023 | biostudies-arrayexpress

Profiling gene expression in 32Dcl3 cells following Xbp1 retrovirus vector transfection

2010-10-01 | GSE17023 | GEO

Adipocyte XBP1s regulates systemic insulin sensitivity by modulating adiponectin multimerization

Project description:The physiological role of the spliced form of X-box-binding protein 1 (XBP1s), a key transcription factor of the endoplasmic reticulum (ER) stress response, in adipose tissue remains largely unknown. Here we show that overexpression of XBP1s promotes adiponectin multimerization in adipocytes, thereby regulating systemic glucose homeostasis. Ectopic expression of XBP1s in adipocytes improves glucose tolerance and insulin sensitivity in both lean and obese (ob/ob) mice. The beneficial effect of adipocyte XBP1s on glucose homeostasis is associated with elevated serum levels of HMW adiponectin and indeed, is adiponectin dependent. Mechanistically, XBP1s promotes adiponectin multimerization rather than activating its transcription likely through a direct regulation of the expression of several ER-chaperones involved in adiponectin maturation, including Grp78, Pdia6, ERp44 and DsbA-L. Thus, we conclude that XBP1s is an important regulator of adiponectin multimerization, which may lead to a new therapeutic approach for the treatment of type 2 diabetes and hypoadiponectinemia. Epididymal adipose tissue from wild type and XBP1-overexpressing mice was subjected to gene expression profiling.

2013-11-21 | E-GEOD-36031 | biostudies-arrayexpress

Androgen-induced modulation of XBP1s is functionally driving part of the AR transcriptional program

Project description:Prostate cancer development and progression is largely dependent on androgen receptor (AR) signaling. AR is a hormone-dependent transcription factor, which binds to thousands of sites throughout the human genome to regulate expression of directly responsive genes, including pro-survival genes that enable tumor cells to cope with increased cellular stress. ERN1 and XBP1 – two key players of the unfolded protein response (UPR) – are among such stress-associated genes. Here, we show that XBP1 levels in primary prostate cancer are associated with biochemical recurrence in five independent cohorts. Patients who received AR targeted therapies had significantly lower XBP1 expression, whereas expression of the active form of XBP1 (XBP1s) was elevated. In vitro results show that AR-induced ERN1 expression led to increased XBP1s mRNA and protein levels. Furthermore, ChIP-seq analysis revealed that XBP1s binds enhancers upon stress stimuli regulating genes involved in UPR processes, eIF2 signaling and protein ubiquitination. We further demonstrate genomic overlap of AR and XBP1s binding sites, suggesting genomic conversion of the two signaling cascades. Transcriptomic effects of XBP1 were further studied by knockdown experiments, which lead to decreased expression of androgen-responsive genes and UPR genes. These results suggest a two-step mechanism of gene regulation, which involves androgen-induced expression of ERN1, thereby enhancing XBP1 splicing and transcriptional activity. This signaling cascade may prepare the cells for the increased protein folding, mRNA decay and translation that accompanies AR-regulated tumor cell proliferation.

2019-12-18 | GSE121880 | GEO

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