Project description:The presence of antibiotic resistance related to Integrative Conjugative Elements (ICEs) in oral streptococci are increasingly being reported. This study investigates the prevalence, diversity and stability of one family of ICEs, Tn916-Tn1545 in blood isolates collected in Norway.
Project description:The presence of antibiotic resistance related to Integrative Conjugative Elements (ICEs) in oral streptococci are increasingly being reported. This study investigates the prevalence, diversity and stability of one family of ICEs, Tn916-Tn1545 in blood isolates collected in Norway.
Project description:Our group recently transcriptomically characterized coculture growth between Streptococcus mutans and several species of commensal streptococci (Rose et al, 2023). However, these experiments were carried out in our lab-based experimental medium, tryptone and yeast extract (TY-). To understand whether culturing these species within a medium that more closely mimics their natural environment alters the interaction, we evaluated both monoculture and coculture growth between the dental caries pathogen Streptococcus mutans and oral commensal species Streptococcus oralis in a half TY- / half human saliva mix that was optimally chosen based on our initial characterization of oral streptococci behaviors in medium mixes containing saliva. Our results surprising show that inclusion of saliva enhances the competition of Streptococcus mutans against commensal streptococci through upregulation of carbohydrate uptake and glycolytic pathways.
Project description:Comparative genomic hybridization of 9 Norwegian E. faecalis baby isolates with E. faecalis V583 as a reference strain using an E. faecalis V583 oligo array. Total gene content was analyzed by whole genome microarrays.
Project description:Comparative genomic hybridisation of Streptococcus pneumoniae isolates from a single clonal complex, in order to determine genomic diversity. Isolates were selected from a range of tissue types and serotypes in order to cover the full diversity of the clone, and also in order to try and identify tissue-specific genes Biological replicates: 19 clonal complex 199 S. pneumoniae isolates. One clonal complex 180 isolate used as an outgroup. Independently grown and isolated. One isolate per array
Project description:Fusobacterium polymorphum has been implicated in oral squamous cell carcinoma, yet its widespread presence in the oral cavity raises questions about whether different clinical isolates exert distinct effects on host cells. To explore host transcriptional responses, we performed transcriptomic profiling of dysplastic oral keratinocytes (DOK) following exposure to F. polymorphum isolates derived from across the oral epithelial disease spectrum. The host transcriptomic response revealed a broadly shared pro-inflammatory signature across all isolates, regardless of their clinical origin. However, expression of genes and pathways associated with cell proliferation and related signaling processes varied among isolates and correlated with the dysplasia severity of the lesions from which the strains were derived. These findings suggest that while F. polymorphum isolates elicit a common inflammatory response in epithelial cells, variation in host transcriptional programs linked to proliferation may reflect differences in their biological impact.
Project description:Campylobacter jejuni has become the predominant cause of sheep abortions in the U.S. However, little is know about the genetic diversity among the isolates collected from different time periods. In this study, the genetic diversity of sheep aborion isolates of C. jejuni was investigated by Array-based CGH
Project description:Campylobacter jejuni has become the predominant cause of sheep abortions in the U.S. However, little is know about the genetic diversity among the isolates collected from different time periods. In this study, the genetic diversity of sheep abortion isolates of C. jejuni was investigated by Array-based CGH
Project description:Comparative genomic hybridisation of Streptococcus pneumoniae isolates from a single clonal complex, in order to determine genomic diversity. Isolates were selected from a range of tissue types and serotypes in order to cover the full diversity of the clone, and also in order to try and identify tissue-specific genes
