Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

A Specific Neuroligin3-αNeurexin1Signaling Regulates GABAergic Synaptic Function in Mouse Hippocampus

ABSTRACT: A Specific Neuroligin3-αNeurexin1Signaling Regulates GABAergic Synaptic Function in Mouse Hippocampus

PROVIDER: PRJNA634328 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR11825846.fastq.gz	Fastqsanger.gz
	SRR11825846_1.fastq.gz	Fastqsanger.gz
	SRR11825846_2.fastq.gz	Fastqsanger.gz
	SRR11825847.fastq.gz	Fastqsanger.gz
	SRR11825847_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 32

Similar Datasets

A Specific Neuroligin3-αNeurexin1Signaling Regulates GABAergic Synaptic Function in Mouse Hippocampus

Project description:Synapses are fundamental organizers of precise signal propagation between neurons. Maintaining synapse assemblies require interactions between pre- and post- synaptic proteins, notably cell adhesion molecules (CAMs). It has been proposed that the function of Neuroligins (Nlgn1 - 4), postsynaptic CAMs, relies on the formation of trans-synaptic complexes with Neurexins (Nrxs), presynaptic CAMs. Nlgn3 is a unique Nlgn isoform that localizes at both excitatory and inhibitory synapses. However, Nlgn3 function mediated through Nrx interaction is mostly unknown. Here, we find for the first time that Nlgn3 localizes at postsynaptic sites apposing vesicular glutamate transporter 3 (VGT3)-expressing inhibitory terminals. Overexpression and knockdown approaches indicate that Nlgn3 regulates VGT3-positive inhibitory interneuron-mediated synaptic transmission. Fluorescent in situ hybridization and single-cell RNA sequencing studies revealed that αNrxn1 and βNrxn3 are VGT3 interneuron-specific Nrxn isoforms and the expression levels of Nrxn splice isoforms are highly diverse in VGT3 interneurons, respectively. Most importantly, postsynaptic Nlgn3 requires presynaptic αNrx1+AS4 expressed in VGT3-positive interneurons to regulate inhibitory synaptic transmission. Our results strongly suggest that specific Nlgn-Nrx interaction generate distinct functional properties at synapses.

2021-01-04 | GSE150989 | GEO

Specific Neuroligin3-αNeurexin1 signaling regulates GABAergic synaptic function in mouse hippocampus.

Project description:Synapse formation and regulation require signaling interactions between pre- and postsynaptic proteins, notably cell adhesion molecules (CAMs). It has been proposed that the functions of neuroligins (Nlgns), postsynaptic CAMs, rely on the formation of trans-synaptic complexes with neurexins (Nrxns), presynaptic CAMs. Nlgn3 is a unique Nlgn isoform that localizes at both excitatory and inhibitory synapses. However, Nlgn3 function mediated via Nrxn interactions is unknown. Here we demonstrate that Nlgn3 localizes at postsynaptic sites apposing vesicular glutamate transporter 3-expressing (VGT3+) inhibitory terminals and regulates VGT3+ inhibitory interneuron-mediated synaptic transmission in mouse organotypic slice cultures. Gene expression analysis of interneurons revealed that the αNrxn1+AS4 splice isoform is highly expressed in VGT3+ interneurons as compared with other interneurons. Most importantly, postsynaptic Nlgn3 requires presynaptic αNrxn1+AS4 expressed in VGT3+ interneurons to regulate inhibitory synaptic transmission. Our results indicate that specific Nlgn-Nrxn signaling generates distinct functional properties at synapses.

| S-EPMC7758064 | biostudies-literature

Neuronal TIMP2 regulates hippocampus-dependent plasticity and extracellular matrix complexity

Project description:The functional output of the hippocampus, a brain region subserving memory processes, depends on highly orchestrated cellular and molecular processes that regulate synaptic plasticity throughout life. The structural requirements of such plasticity and molecular processes involved in this regulation are poorly understood. Specific molecules, including tissue inhibitor of metalloproteinases-2 (TIMP2) have been implicated in serving a pro-plasticity role in the hippocampus, a role that decreases with brain aging. Here, we report that TIMP2 is highly expressed by neurons within the hippocampus and its loss drives changes in cellular programs related to adult neurogenesis and dendritic spine turnover with corresponding impairments in hippocampus-dependent memory. We find that TIMP2 regulates accumulation of extracellular matrix (ECM) around synapses in the hippocampus with concomitant hindrance in migration of newborn neurons through a denser ECM network. A conditional TIMP2 KO mouse reveals that neuronal TIMP2 regulates adult neurogenesis, accumulation of ECM, and ultimately hippocampus-dependent memory. Our results define a mechanism whereby hippocampus-dependent function is regulated by TIMP2 and its interactions with the ECM to regulate diverse processes associated with synaptic plasticity.

2023-11-02 | GSE223188 | GEO

Rapid conversion of fibroblasts into functional forebrain GABAergic interneurons by direct genetic reprogramming

Project description:Transplantation of GABAergic interneurons (INs) can sustain long-standing benefits in animal models of epilepsy and other neurological disorders. In a therapeutic perspective, a renewable source of functional GABAergic INs is needed. Here, we identified five factors (Foxg1, Sox2, Ascl1, Dlx5 and Lhx6) able to convert fibroblasts directly into induced GABAergic INs (iGABA-INs), displaying the molecular signature of telencephalic INs. The selected factors recapitulate in fibroblasts the activation of transcriptional networks required for the specification of GABAergic fate during telencephalon development. iGABA-INs exhibited progressively maturing firing patterns comparable to those of cortical INs, had synaptic currents and released GABA. Importantly, upon grafting in the hippocampus, iGABA-INs survived, matured and their optogenetic stimulation triggered GABAergic transmission and inhibited the activity of connected granule cells. The five factors also converted human cells into functional GABAergic neurons. These properties define iGABA-INs as a promising tool for disease modeling and cell-based therapeutic approaches. Comparison of iGABA-INs transcriptional profile with those of starting fibroblasts and GAD67-GFP+ cortical interneurons.

2015-12-15 | E-GEOD-74065 | biostudies-arrayexpress

SFRS8 regulates memory by modulating pre-mRNA splicing of synaptic genes in mice

Project description:SFRS8 is a member of SR family protein. It is also a known splicing factor that is highly expressed in the brain. However, its function in the brain remains elusive. Here, we find that SFRS8 is required to maintain normal synaptic protein levels as well as synaptic densities. Mechanistically, we demonstrate that SFRS8 interacts with SF3B3, a major component of U2 snRNP complex, and regulates the RNA alternative splicing process. Specifically, SFRS8 regulates the levels of PSD95, through enhancing the loading of Psd95 mRNA on the U2 snRNP complex and increasing the skipping of exon18 of Psd95. We further demonstrate that SFRS8 knockdown in the hippocampus leads to reduced synaptic protein expression, decreased spine density, consequently defective memory in mice. Similar reduction in synaptic protein expression and synaptic density are also evident in cultured neurons. Taken together, SFRS8 regulates memory function in mice through altering the alternative splicing and expression of synaptic genes via SFRS8-SF3B3 interaction.

2025-06-06 | GSE273869 | GEO

Pumilio regulates synaptic protein function in a 3’UTR-dependent manner

Project description:Pumilio regulates synaptic protein function in a 3’UTR-dependent manner

| PRJNA976181 | ENA

Pumilio regulates synaptic protein function in a 3’ UTR-dependent manner

Project description:Pumilio regulates synaptic protein function in a 3’ UTR-dependent manner

| PRJNA1213792 | ENA

Pumilio regulates synaptic protein function in a 3’UTR-dependent manner [Pum_3RIPseq]

Project description:Pumilio regulates synaptic protein function in a 3’UTR-dependent manner [Pum_3RIPseq]

| PRJNA976183 | ENA

Pumilio regulates synaptic protein function in a 3’ UTR-dependent manner[primary_neuron_RNASeq]

Project description:Pumilio regulates synaptic protein function in a 3’ UTR-dependent manner[primary_neuron_RNASeq]