Project description:We analysed the transcriptomic response of 3 rhizobial symbionts of Mimosa pudica (Rhizobium mesoamericanum STM3625, Cupriavidus taiwanensis LMG19424 and Burkholderia phymatum STM815) when cultivated in a minimum culture medium (control condition) versus induced by root exudates of their host plant Mimosa pudica. We used RNAseq using illumina technology.
Project description:Priming is a mechanism used by plants to rapidly respond to external stimuli, becoming essential for their survival and resistance to unfavorable conditions. The physiological state acquired by a plant after the exposure to repeated stress is the result of molecular mechanisms determining plant’s memory of past stress events. Mimosa pudica is a plant known for its ability to respond to physical disturbance by folding its leaves. The movement is determined by a strong Ca2+ influx, which acts as both osmotic pressure regulator and secondary messenger for gene expression. Considering the need to intervene in a sustainable way to counteract the ever-increasing biotic and abiotic stresses caused by climate change, it would be particularly interesting to deepen our knowledge on stress tolerance development in primed plants. From this perspective, M. pudica can be a valuable model for studying the molecular mechanisms related to adaptive memory acquisition after repeated stress events. In this study, the gene regulatory networks underlying M. pudica leaf closure following single and multiple mechanical disturbances (droppings) were investigated.
Project description:We first report the use of next-generation massively parallel sequencing technologies and de novo transcriptome assembly to gain insight into the wide range of transcriptome of Hevea brasiliensis. The output of sequenced data showed that more than 12 million sequence reads with average length of 90nt were generated. Totally 48,768 unigenes (mean size = 488 bp) were assembled through transcriptome de novo assembly, which represent more than 3-fold of all the sequences of Hevea brasiliensis deposited in the GenBank. Assembled sequences were annotated with gene descriptions, gene ontology and clusters of orthologous group terms. Total 37,373 unigenes were successfully annotated and more than 10% of unigenes were aligned to known proteins of Euphorbiaceae. The unigenes contain nearly complete collection of known rubber-synthesis-related genes. Our data provides the most comprehensive sequence resource available for study rubber tree and demonstrates the availability of Illumina sequencing and de novo transcriptome assembly in a species lacking genome information. The transcriptome of latex and leaf in Hevea brasiliensis
Project description:We combined multi-omics approaches including de novo transcriptome assembly, ribosome profiling and MS-based peptidomics to study the global role of mRNA translation and small ORFs (sORFs) in rice herbicide resistant mutant.