Project description:Arabidopsis polyadenylation factor PCFS4 functioned in the alternative polyadenylation of FCA. To identify additional genes differetially processed by PCFS4, we compared the transcriptomes of PCFS4 mutant, pcfs4-1 and wild type Col with Arabidopsis tiling array analyses and a newly developed data processing protocol, RADPRE. We found 68 genes were differentially processed and 114 genes differetially expressed in pcfs4-1 mutant. The top 7 of 68 differetially processed genes were further confirmed with real-time RT-PCR analysis.
Project description:The sid2.2 mutant (Dewdney et al. 2000) with deletion/arrangement in exon 9 is deficient for salicylic acid synthesis. We used Affymetrix microarrays to detail the global programme of gene expression underlying metabolic and cellular changes in the rosettes of the Arabidopsis sid2.2 mutant and wild type grown under strong sulfate limitation.
Project description:ARABIDOPSIS SKP1-LIKE1 (ASK1) protein is involved in regulating flower development. We have compared ask1 mutant floral transcriptome with wild-type Ler to identify the role of ASK1-containing E3 ubiquitin ligases in regulating flower transcriptome. In this dataset, we include the expression data obtained from Arabidopsis thaliana Ler and ask1 mutant flower buds. We identified 42 genes and 74 genes that are down-regulated and up-regulated, respectively.
Project description:Glycolytic Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) catalyzes the conversion of glyceraldehyde 3-phospate to 1,3-bisphosphoglycerate by coupling with the reduction of NAD+ to NADH. Both cytosolic and plastidial isoforms of GAPDH has been described but the in vivo functions of the plastidial isoforms is unresolved. We generated mutants of the Arabidopsis plastidial GAPDH isoforms (At1g79530, At1g16300; GAPCp1, GAPCp2) and performed a microarray analysis comparing gapcp double (gapcp1 gapcp2) mutant and wild type seedlings