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RNAseq anaysis of entorhinal cortex layer II neurons upon Ptbp1 modulation

ABSTRACT: RNAseq anaysis of entorhinal cortex layer II neurons upon Ptbp1 modulation

PROVIDER: PRJNA635608 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR11863035_1.fastq.gz	Fastqsanger.gz
	SRR11863035_2.fastq.gz	Fastqsanger.gz
	SRR11863036_1.fastq.gz	Fastqsanger.gz
	SRR11863036_2.fastq.gz	Fastqsanger.gz
	SRR11863037_1.fastq.gz	Fastqsanger.gz

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RNAseq anaysis of entorhinal cortex layer II neurons upon Ptbp1 modulation

Project description:Purpose: To identify gene expression changes in entorhinal cortex layer II (ECII) neurons upon Ptbp1 modulation (silencing and overexpression) Method: bacterial artificial chromosome - Translating Ribosome Affinity Purification (bacTRAP) to isolate actively translated mRNA in ECII neurons, 2 weeks after stereotaxic injection of an AAV1 vector in the EC of ECII-bacTRAP mice; followed by RNAseq. Note: Ptbp1 was significantly overexpressed in the overexpression experiment, but no silencing was achieved with the silencing vector, probably because of tight control of Ptbp1 expression

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