Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

RNA-seq of CBD-treated SK-N-BE(2) cells over a time course of 3hr - 24hr with vehicle (ethanol) matched controls at each time point.

ABSTRACT: RNA-seq of CBD-treated SK-N-BE(2) cells over a time course of 3hr - 24hr with vehicle (ethanol) matched controls at each time point.

PROVIDER: PRJNA636172 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR11884800.fastq.gz	Fastqsanger.gz
	SRR11884900.fastq.gz	Fastqsanger.gz
	SRR11884801.fastq.gz	Fastqsanger.gz
	SRR11884901.fastq.gz	Fastqsanger.gz
	SRR11884802.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 224

Similar Datasets

RNA-seq of CBD-treated SK-N-BE(2) cells over a time course of 3hr - 24hr with vehicle (ethanol) matched controls at each time point.

Project description:Cannabidiol (CBD) is an FDA approved drug for treatment of drug-intractable forms of pediatric epilepsy. While a unified mechanism of action has yet to emerge from numerous proposed molecular targets of CBD, preclinical studies reveal a pleiotropic pharmacology. Here, we report the application of temporally-resolved multi-omic profiling and biosensor screening to dissect the molecular mechanism of CBD in human neuroblastoma cells. CDB treatment led to a rise in cytosolic calcium and activation of AMPK signaling within 2 hours. Subcellular profiling of proteins, metabolites, and mRNA transcripts identified CBD-dependent activation of cholesterol biosynthesis, transport and storage. We found that CBD incorporates into cellular membranes, alters cholesterol activity, and increases lipid order. We also report CBD-induced apoptosis in multiple human cell lines, which is rescued by statins and potentiated by compounds that disrupt cholesterol trafficking and storage. Our data point to pharmacological interaction of CBD with cholesterol homeostasis pathways, with potential implications in its therapeutic use.

2020-06-01 | GSE151512 | GEO

ATAC-seq analysis for DND-41 and MOLT4 cells with ACBI-1 treatment

Project description:ATAC-seq analysis was performed in two T-ALL cell lines (DND-41 and MOLT-4) with either DMSO or ACBI-1 treatment at two different time points (3hr and 24hr).

2024-07-02 | GSE230584 | GEO

Elucidating the transcriptomic response of adult hypothalamic neuron cells to cannabidiol (CBD) exposure

Project description:Cannabidiol (CBD) actions in the brain are largely complex and not fully understood. One important brain structure that is a target for CBD, and for which health-beneficial CBD consequences can be seen is the hypothalamus. Given the fundamental role of gene expression in the hypothalamus's regulatory functions, this study undertook the analysis of changes arising in hypothalamic cells’ transcriptomes following various CBD treatments. For this purpose, we used a hypothalamic cellular model, namely we employ adult-derived mHypoA-2/12 mouse cell lines. In the course of the study, the neural cells were treated with different CBD doses (ranging from 0.325 to 3 µM) and vehicle as a control for 6 and 24 hours. The experiment setup allowed us to evaluate both the dosage and time-dependent effect of CBD on hypothalamic cells, particularly on their viability, apoptosis, and transcriptome profile.

2024-08-01 | GSE270378 | GEO

Canabidiol (CBD) modulates the effect of ethanol on the fibroblast cells transcriptome in vitro

Project description:Considering reports about positive role of Cannabidiol (CBD) in alcohol abuse disorders, in this study we used cellular model of human fibroblasts to analyse the effect of CBD on ethanol-treated cells. We assumed, that CBD will (to some extent), prevent adverse transcriptional changes driven by alcohol administration and this effect will be detectable on the transcriptome level. As a by-product of this analysis, ethanol and CBD alone stimulated transcriptome changes were also described. We moreover considered that CBD effects are dose and time-dependent. As a model for these analyses we selected human fibroblast cell line as a most abundant cell type in human body that express e.g. both cannabinoid receptors (CB1 and CB2) and is able to metabolize ethanol trough alcohol dehydrogenase activity.

2023-08-01 | GSE232624 | GEO

Effects of CBD on the glioma stem cell molecular signature

Project description:Primary glioma stem cells cultured as neurospheres in NBL media with growth factors were subjected to treatment with the non-toxic, non-psychoactive cannabis compound cannabidiol (CBD). Control and CBD- treated cultures were used to generate RNA used to hybridize on Affymetrix DNA arrays Fluorescence intensities data were RMA-normalized using Partek software.

2015-04-17 | E-GEOD-57978 | biostudies-arrayexpress

cbd-1

Project description:Caenorhabditis elegans cbd-1, Chitin-binding domain protein cbd-1 [Source:UniProtKB/Swiss-Prot;Acc:O45599], is expressed in 1 baseline experiment(s);

| WBGene00010351 | ExpressionAtlas

cbd-1

Project description:Caenorhabditis elegans cbd-1, Chitin-binding domain protein cbd-1 [Source:UniProtKB/Swiss-Prot;Acc:O45599], is differentially expressed in 7 experiment(s);

| WBGene00010351 | ExpressionAtlas

Elucidating The Role Of ∆9-Tetrahydrocannabinol (Thc) And Cannabidiol (Cbd) In Developmental Toxicity

Project description:Increased availability of cannabis and cannabinoid-containing products necessitates the need for understanding how exposure to these compounds can affect development. Using cannabinoid receptor-null zebrafish (cnr1-/- and cnr2-/-), we conducted experiments to assess the roles of these receptors in ∆9-tetrahydrocannabinol (THC) and cannabidiol (CBD) developmental and behavioral toxicity. THC increased mortality and deformities (pericardial and yolk sac edemas, a reduction in size) in cnr1-/- and cnr2-/- fish. Conversely, CBD-induced malformations and mortality were significantly reduced in the cnr1-/- and cnr2-/- larvae. THC and CBD exposure caused significantly decreased larval behavior (96 hpf), however, decreased distance travelled was protected in the cnr1-/- and cnr2-/- fish, suggesting these receptors are responsible for mediating behavioral toxicity. Transcriptomic profiling in cnr+/+ embryos developmentally exposed to 4 μM THC or 0.5 μM CBD revealed that a significant portion of differentially expressed genes were targets of PPARγ, a predicted upstream regulator. In Cnr-positive embryos, co-exposure to the PPARγ inhibitor GW9662 and THC or CBD, there was increased toxicity compared to exposure with THC or CBD alone. Co-treatment in the cnr2-/- fish with GW9662 did not alter the CBD-induced decrease in activity. However, co-treatment with GW9662 did remove the protective effect observed in cnr1-/- fish treated to CBD alone. Collectively, these results indicate that PPARγ, Cnr1, and Cnr2 all play crucial roles in the developmental toxicity of THC and CBD.

2021-01-01 | GSE164128 | GEO

Effects of CBD on the glioma stem cell molecular signature

2015-04-17 | GSE57978 | GEO

WT and aP2-Cre Adipocyte Bmal1 KO 24hr Time Course, Liver Tissue RNA-seq

Project description:WT and aP2-Cre Adipocyte Bmal1 KO 24hr Time Course, Liver Tissue RNA-seq

| PRJNA474430 | ENA