Ontology highlight
ABSTRACT:
PROVIDER: PRJNA636927 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Genome sequencing of actinomycetes for natural products discovery
| PRJNA393980 | ENA
Project description:Gram-negative bacteria are an emerging source of natural products with pharmaceutical potential. However, the limited availability of essential genetic tools for drug discovery and sustainable production remains a challenge. Burkholderia serves as a promising source for such tools, as it produces diverse natural products and can be experimentally manipulated in the laboratory. Hence, this study aimed to identify strong and constitutive promoters from Burkholderia stagnalis TBRC 18363 for unlocking the potential of cryptic biosynthetic genes and enhance natural product production. Highly expressed genes were identified through transcriptomic analysis. Twenty-six promoters were evaluated in Escherichia coli and Pseudomonas putida reporter systems. Among all, promoters p2035 and p5642 exhibited superior performance in both systems. These promoters were selected for further investigation and were found to enhance the production titer of icosalide in B. stagnalis TBRC 18363 and FR900359, a Gq/11 protein inhibitor depsipeptide, in Chromobacterium vaccinii. This research emphasizes efficient promoters for target gene overexpression in Gram-negative bacteria.
2025-10-31 | GSE269653 | GEO
Project description:For peloid natural product discovery we have produced 12 spectral libraries, 6 for nighttime sampled peloid (.mgf libraries containing suffix "N") and 6 for daytime sampled peloid (.mgf libraries containing suffix "D" in their name), containing a total of 14,417 individual mass spectra, using a Quadrupole Time-of-Flight (qTOF; 6546 LC/Q-TOF, Agilent, USA) LC-HRMS. In order to identify known natural products, preferably with predetermined biological activity, which would enable us to evaluate Nin peloid mud medicinal property claims, we have used freely available COlleCtion of Open Natural prodUcTs (COCONUT).
2021-11-24 | MSV000088445 | MassIVE
Project description:Natural products exhibit potential as candidates for developing multi-target agents for Alzheimer's disease treatment. The aim of this study is to utilize network-based medicine to identify novel natural products for Alzheimer's disease, and investigate their efficacy and mechanisms of action. In this study, we identified (-)-Vestitol and Salviolone as new potential natural products for treating Alzheimer's disease via an Alzheimer's disease-related pathway-gene network. Both natural products improved the cognition of APP/PS1 transgenic mice, reduced Aβ deposition, and lowered soluble toxic Aβ levels in the brain. Notably, a synergistic effect was observed when the two natural products were combined. Transcriptomic analysis and qRT-PCR experiments revealed that the synergistic mechanism of (-)-Vestitol and Salviolone combination is associated with the regulation of a broader range of AD-related pathways and genes, particularly the neuroactive ligand-receptor interaction pathway and calcium signaling pathway.
2025-02-19 | GSE270100 | GEO
Project description:Reeindeer lichens as potential natural products source
| PRJEB34718 | ENA