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Single-cell transcriptome analysis of endothelial cell heterogeneity under laminar shear stress

ABSTRACT: Single-cell transcriptome analysis of endothelial cell heterogeneity under laminar shear stress

PROVIDER: PRJNA637536 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR11932654.fastq.gz	Fastqsanger.gz
	SRR11932655.fastq.gz	Fastqsanger.gz
	SRR11932656.fastq.gz	Fastqsanger.gz
	SRR11932657.fastq.gz	Fastqsanger.gz
	SRR11932658.fastq.gz	Fastqsanger.gz

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Single-cell transcriptome analysis of endothelial cell heterogeneity under laminar shear stress

Project description:Heterogeneity of endothelial cells that form the innermost layer of all vessels is critical for vascular sprouting and angiogenesis. After new vessels form, endothelial cell heterogeneity is believed to be gradually lost as vessels respond to flow-mediated signals, mature, remodel and become homeostatic. However, whether and at what level endothelial cell lost heterogeneity is poorly understood. Here we investigated heterogeneity change of endothelial cells under homeostatic laminar shear tress in comparison to static cultures.

2021-05-25 | GSE151867 | GEO

HUVECs cultured under normal or high laminar shear stress

Project description:Many studies have characterised the effect of normal laminar shear stress (LSS) on endothelial responses, however elevated shear stress, as would be experienced overlying a stenotic plaque, has not been studied in depth. Therefore we used transcriptomics and related functional analyses to compare cells exposed to laminar shear stress at 15 or 75 dynes/cm2 for 24 hours (LSS15-normal or LSS75-high shear stress). Human umbilical vein endothelial cells (HUVEC n=4 per flow condition from different batches of pooled donor HUVEC p2) were cultured for 24 hours on gelatin coated slides under laminar shear stress of either 15 dynes/cm2 or 75 dynes/cm2 (LSS15 or LSS75) to assess the effect of high shear stress on endothelial cells. Total RNA was obtained using Qiagen kit and array analysis performed by Service XS (Leiden, Netherlands).

2011-02-01 | E-GEOD-23289 | biostudies-arrayexpress

HUVECs cultured under normal or high laminar shear stress

2011-02-01 | GSE23289 | GEO

Endothelial Cell Cycle Responses to Laminar Shear Stress

Project description:Endothelial Cell Cycle Responses to Laminar Shear Stress

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Transcription profiling by array of human young and senescent HUVECs under static and laminar shear stress conditions

Project description:Laminar shear stress due to constant blood flow is known to play a critical role in maintaining vascular health. In contrast, endothelial cell senescence appears to be closely associated with the incidence of vascular disorder. In an attempt to identify functional biomarkers for age-related vascular health/disease, the present study investigated differential gene expression of young and senescent human umbilical vein endothelial cells (HUVECs) under static and laminar shear stress. We used a cDNA microarray method to compare gene expression profiles of young and senescent HUVECs under static and laminar shear stress conditions. Experiment Overall Design: Senescent cells were prepared by continuous subculture in vitro, and a cone-and-plate device was used to impose laminar shear stress onto cells. Young and senescent cells were exposed to laminar shear stress or maintained under static conditions. Total mRNA was extracted and gene expression profiles were analyzed by cDNA microarray.

2009-06-01 | E-GEOD-13712 | biostudies-arrayexpress

Vascular-type heterogeneity is associated with differential gene expression profiles of endothelial cells under shear stress

Project description:Background: Endothelial cell (ECs) heterogeneity is an emerging area of research in EC biology. Veins, arteries, and microvascular endothelial cells, termed “vascular-type” heterogeneity in this report, have been shown to have heterogeneity in gene expression and function. In addition to this innate heterogeneity, we hypothesized that different vascular-type ECs would also demonstrate heterogeneity in their response to shear stress. Objectives: We interrogated whether vascular-type ECs would demonstrate variations in transcriptional expression patterns under shear stress. Methods: Human umbilical vein endothelial cells (HUVECs), human pulmonary arterial endothelial cells (HPAECs), and human microvascular endothelial cells (HMVECs) were commercially purchased and subjected to shear stress conditions of 0, 1, 4, and 10 dynes/cm2 of laminar shear stress. After exposure to shear stress, cells were analyzed for cellular alignment and RNA was extracted and evaluated via bulk RNA-sequencing (RNA-seq). Results: All ECs demonstrated significant changes in alignment under shear stress. Shear stress significantly affected the transcriptomes of ECs resulting in differentially expressed genes and pathways; while several genes were differentially expressed across all three vascular-types (44.2%), most differentially expressed genes were limited to 1 or 2 of the vascular-types. Hemostatic and thrombotic genes were found to have differential expression patterns under conditions of shear stress, and VWF demonstrated a pattern of vascular-type heterogeneity in response to shear stress. Conclusions: Shear stress causes changes in cellular alignment and transcriptional patterns in ECs that is dependent upon underlying vascular-type. Therefore, endothelial vascular-type heterogeneity can regulate response to shear stress, especially in hemostatic and thrombotic gene expression.

2025-07-23 | GSE294621 | GEO

Differential gene expression of young and senescent HUVECs under static and laminar shear stress conditions

2009-05-23 | GSE13712 | GEO

CircRNA profiling in laminar shear stress-treated human umbilical vein endothelial cells.

Project description:Laminar shear stress (LSS) suppresses endothelial inflammation and protects the arteries from atherosclerosis. Circular RNAs (circRNAs) are powerful regulators of vascular homeostasis and atherosclerosis; however, their roles in mediating the effects of LSS remain unexplored. To identify the changes in circRNA expression patterns after shear stress stimulation, we conducted circRNA microarray analysis using RNA extracted from HUVECs cultured for 24 h under static or LSS conditions.

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Genome-wide analysis of mechano-responsive gene expression of mesenchymal stem cells after laminar shear stress

Project description:Analysis of mechano-regulation of mesenchymal stem cell gene expression level. The hypothesis tested in the present study was that laminar shear stress influences the cytoskeleton arrangement and amount and then triggers seriers of cell metabolism and cellular functions. Results provide important information of the response of mesenchymal stem cell to laminar shear stress, such as specific mechano-responsive genes, up- or down-regulated specific anabolic/catabolic cellular functions.

2018-12-31 | GSE109180 | GEO

Transcriptome analysis of the role of SMAD6 in mediating endothelial cell response to laminar shear stress by high throughput RNA sequencing

Project description:Laminar shear stress regulates blood vessel morphogenesis and subsequent quiescence, but how endothelial cells (EC) enact and maintain the vascular homeostasis required in most vessels for proper vessel function is poorly understood. SMAD6, a scaffold for several signaling pathways, is expressed in developing arteries and its expression is flow-regulated. We found that SMAD6 is essential for endothelial cell flow-mediated responses, and that it functions downstream of the mechanosensor Notch1. Endothelial cells with reduced SMAD6 levels failed to align under stable laminar shear flow that promotes vascular homeostasis, while forced SMAD6 expression rescued misalignment induced by reduced Notch1 signaling. SMAD6-dependent homeostatic laminar flow required the Notch ligand Dll4 and Notch transcriptional activity. Mechanistically, neither the N-terminal nor the C-terminal domain of SMAD6 alone rescued flow alignment upon loss of Notch signaling. Endothelial cells with reduced Smad6 levels has compromised barrier function, and RNA profiling revealed upregulation of proliferation-associated genes and down regulation of junction-associated genes. Among junction-related genes affected by SMAD6 levels, the proto-cadherin PCDH12 was upregulated by homeostatic flow and required for proper flow-mediated endothelial cell alignment. Thus, SMAD6 is a critical integrator of flow-mediated signaling inputs downstream of Notch1, as vessels transition from an angiogenic to a homeostatic phenotype.

2021-03-29 | GSE147036 | GEO

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