Molecular interactions at the bovine embryo–endometrial epithelium interface
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA637537 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| SRR11932700.fastq.gz | Fastqsanger.gz | |||
| SRR11932701.fastq.gz | Fastqsanger.gz | |||
| SRR11932702.fastq.gz | Fastqsanger.gz | |||
| SRR11932703.fastq.gz | Fastqsanger.gz | |||
| SRR11932704.fastq.gz | Fastqsanger.gz |
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Project description:We aimed to interrogate the embryo–maternal interface for embryo-induced changes in the global transcriptome of bovine endometrial epithelial cells (bEECs). In vitro-produced day-5.5 morulae were cultured in juxtacrine apposition (Contact+) with bEECs, or in transwells (Contact⎼), for 48 h. A group of bEECs cultured in the absence of embryos (Control bEEC) was also included. After co-culture, bEECs were recovered for transcriptome analysis.
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Project description:This SuperSeries is composed of the following subset Series: GSE20974: Bovine pre-transfer endometrium and embryo transcriptome fingerprints as predictors of pregnancy success after embryo transfer (endometrial study) GSE21047: Bovine pre-transfer endometrium and embryo transcriptome fingerprints as predictors of pregnancy success after embryo transfer (embryo study) Refer to individual Series
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Project description:Extracellular vesicles (EVs) released by cells contain mRNAs, miRNAs, lncRNAs, lipids, and proteins, playing crucial roles in cell-cell communication. While full-length mRNA transcripts have been documented in EVs secreted by cancer cells, there are no reports on full transcripts secreted by embryos. Our study aimed to identify EV mRNAs in the culture media of bovine embryos and investigate their roles in embryo-mother communication. Following the isolation of EVs from in-vitro fertilization media samples and RNA sequencing, we identified a full mRNA transcript of DPPA3, known to play an essential role in embryo development. To examine the role of DPPA3 in embryo-mother communication, an in vitro transcribed mRNA of DPPA3 was transfected into bovine endometrial epithelial cells. Transfected and control cells were subsequently analyzed with RNA sequencing and proteomics to assess the effects of DPPA3 on gene expression. A total of 24 genes were found to be upregulated, and one gene was downregulated (FDR < 0.01) following DPPA3 transfection, many with known functions in pregnancy recognition. Proteomic analysis revealed 28 differentially expressed, with 19 upregulated and 15 downregulated. Two proteins, ISG15 and MX1, overlapped with the differentially expressed mRNAs. To mimic the natural transfer of EVs from embryos to endometrial cells, we performed co-culture with day-8 blastocysts or supplemented the cells with embryo-conditioned culture media. DPPA3 presence was detected in endometrial cells exposed to embryo-conditioned media after just 30 minutes. Overall, our study highlights the significant role of EVs in cell-cell communication through mRNA signaling from the embryo to the mother.
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Project description:- Endometrial sEV protein cargo are hormonally regulated – EP treated sEVs are enriched in key players of embryo implantation - Endometrial sEVs are taken up by recipient trophectoderm cells - Endometrial EP-sEVs (or secretory phase sEVs) promote trophectoderm cell invasion via MAPK signalling pathway - Invasion-related proteins are enriched on Tsc cell surface following EP-sEV treatment
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Project description:Successful embryo implantation requires coordinated changes in the uterine luminal epithelium, including structural alterations, apical-basal polarity shifts, intrauterine fluid resorption, and cellular communication. These processes, collectively termed plasma membrane transformation (PMT), are crucial but poorly understood, especially regarding their regulation by planar cell polarity (PCP) proteins. PCP proteins, essential for cell organization, are understudied in the context of uterine physiology. Prickle proteins, particularly Prickle1, play critical roles in cellular polarization and tissue morphogenesis. However, their function in the endometrial epithelium during embryo implantation is unknown. To investigate Prickle1's role in uterine physiology, we developed an endometrial epithelial conditional knockout (cKO) of Prickle1 using Lactoferrin-icre. PRICKLE1 ablation in the endometrial epithelium resulted in decreased fertility and embryo implantation by gestational day 4.5. Three-dimensional imaging revealed abnormal luminal folding, impaired luminal closure, and altered glandular structure in mutant uteri. Additionally, we observed decreased aquaporin-2 expression, disrupted cellular architecture, and protein expression and localization in mutant uteri. Evidence of epithelial-to-mesenchymal transition (EMT) was found within luminal epithelial cells, further linking PRICKLE1 loss to uterine pathologies. Furthermore, altered cellular division planes and increased multinucleated cells suggested a connection to EMT. Our findings highlight PRICKLE1's critical role in the PCP pathway within the uterus, revealing its importance in the molecular and cellular responses essential for successful pregnancy establishment.
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