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Differential expression of endogenous retroelements (long terminal repeats) in ADA2 depleted primary endothelial cells (HUVECs)

ABSTRACT: Differential expression of endogenous retroelements (long terminal repeats) in ADA2 depleted primary endothelial cells (HUVECs)

PROVIDER: PRJNA637655 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR11939103_1.fastq.gz	Fastqsanger.gz
	SRR11939103_2.fastq.gz	Fastqsanger.gz
	SRR11939104_1.fastq.gz	Fastqsanger.gz
	SRR11939104_2.fastq.gz	Fastqsanger.gz
	SRR11939105_1.fastq.gz	Fastqsanger.gz

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Differential expression of endogenous retroelements (long terminal repeats) in ADA2 depleted primary endothelial cells (HUVECs)

2020-06-20 | GSE151924 | GEO

The role of the central region of Ada2 in gene regulation

Project description:The SAGA complex of Saccharomyces cerevisiae contains greater than 20 components that acetylate and deubiquitylate nucleosomal histones. Its acetyltransferase, Gcn5 preferentially acetylates histones H3 and H2B and is regulated through interactions with Ada2 and Ngg1/Ada3. The N-terminal region of Ada2 contains a SANT domain that contacts Gcn5 near its catalytic site. Sequence alignments of Ada2 homologues indicate a conserved ~120 amino acid residue central region that interacts with Ngg1.To examine the function of this central region, we constructed ada2 alleles with mutations of clustered conserved residues. One of these alleles, ada2-RLR, resulted in a ~3-fold reduction in transcriptional activation of the PHO5 gene and growth changes that parallel deletion of ada2. Microarray analyses further revealed that ada2-RLR alters expression of a subset of those genes affected by deletion of ada2. Keywords: yeast, Ada2, SAGA complex, gene expression, genetic modification

2008-11-11 | GSE11377 | GEO

Impact of ADA2 on human macrophages

Project description:This study aims to understand the impact of adenosine deaminase 2 (ADA2) on human monocyte-derived macrophages

2021-09-24 | GSE184572 | GEO

Tanscriptional profile of primary endothelial cells (HUVEC) in ADA2 depleted setting and effect of Dipyridamole (DPM) treatment

Project description:Adenosine deaminase 2 deficiency (DADA2) is an inherited disorder which can cause vasculitis of medium-sized blood vessels. The disease is caused by mutations in the CECR1 gene, which encodes a key metabolic enzyme in the purine pathway. It is not clear how DADA2 leads to vasculitis, and there are currently limited treatment options. We, therefore, as a first step aimed to study the altered gene expression profile of primary endothelial cells in ADA2 depleted setting and then assessed the effect of Dipyridamole (DPM), a pharmacological inhibitor of ENT- transporters, on the differentially expressed genes. The whole genome transcriptome analysis revealed a robust induction of an IFNβ-stimulated gene signature in unstimulated siADA2-treated cells. Differentially expressed transcripts in this dataset included genes encoding pro-inflammatory cytokines, chemokines, and innate immune response proteins. Pre-treatment with dipyridamole (DPM), reduced expression of the IFNβ-driven gene signature upon depletion of ADA2.

2020-06-01 | GSE150540 | GEO

Transcriptional network controlled by Candida glabrata Ada2, a transcription coactivator

Project description:In order to identify genes governed by Ada2, a transcription coactivator in C. glabrata, we compared the genome-wide expression profile in wild-type strain CBS138 and ada2 mutant via RNA sequencing. According to our analyses, 43 genes were down- and 443 genes were up-regulated in ada2 mutant when compared with the wild-type. The 43 down-regulated genes involved in ergosterol biosynthesis pathway (ERG6, ERG13), adhesin or adhesin-like genes (ICS2, AWP2, EPA7), heat shock genes (HSP30, SSB2) and other cellular functions. Among the 443 up-regulated genes, nine adhesin-like EPA family genes (EPA1, EPA6, EPA8, EPA10, EPA11, EPA12, EPA15, EPA20, EPA23) and three yapsin family genes (YPS4, YPS8, YPS10) were included.

2017-05-24 | GSE76338 | GEO

Impact of ADA2 on human macrophages

Project description:Impact of ADA2 on human macrophages

| PRJNA765187 | ENA

Next Generation Sequencing of HUVECs stimulated by conidia of Aspergillus fumigatus

Project description:Purpose: The goal of this study is to investigate the responses of HUVECs after the stimulation of conidia of A. fumigatus Methods: HUVECs were stimulated with conidia of Aspergillus fumigatus for 2 and 6 hours. Three biological repeats of stimulated cells or un-stimulated controls were send for RNA sequencing. Results: Using an optimized data analysis workflow, we mapped about 40 million sequence reads per sample to the human genome (build hg38) and identified round 80,000 transcripts in the HUVECs upon stimulation. Conclusions: Our resutls showed the detailed analysis of HUVECs transcriptomes upton conidia of Aspergillus fumigatus stimulation.

2022-03-29 | GSE191240 | GEO

GCN5-ADA2 Recruits ATP-Citrate Lyase (ACL) and Forms Condensates to Modulate Acetylation at H3K9 for Functional Genes in Rice Meristem

Project description:The SAGA complex is a highly conserved histone acetyltransferase (HAT) complex in eukaryotes, playing crucial role in regulating gene transcription throughout organismal development. The complex consists of two core components: GCN5, the HAT subunit, and ADA2, which primarily functions as an adaptor, facilitating interactions between GCN5 and other proteins and enhancing the complex’s accessibility to nucleosomes. Beyond its well-established roles, we discovered that, in rice, ADA2 possesses an evolutionary conserved intrinsically disordered region (IDR) that directs the SAGA complex to form nuclear condensates. Moreover, we identified a physical interaction between ADA2 and ATP-citrate lyase subunit A2 (ACLA2), leading to the formation of a GCN5-ADA2-ACLA2 (GAA) complex. Both GCN5 and ACLA2 exhibit phase separation in vivo, a process that is dependent on ADA2. Within these condensates, ACL contributes to the production and enrichment of acetyl-CoA, thereby promoting histone acetylation. Genetic evidence showed that knock-out or suppression of the three indicated genes collectively resulted in diminished meristem zones at both root tips and inflorescences, accompanied by a significant reduction in genomic H3K9 acetylation and transcriptional attenuation of essential genes for meristem function. In summary, our findings unveiled a novel mechanism for HAT in modulating gene transcription and plant development, in which the GAA complex phase separates and enriches acetyl-CoA metabolites within the nuclear puncta to facilitate the acetylation of histones at target genes.

2025-09-23 | GSE300491 | GEO

DNAJA1 KO on HUVECs with heat stress

Project description:HS-induced proteome changes in WT and DNAJA1-KO cells were quantified using label free-based quantitative proteomic analysis. Four groups:wild type HUVECs group, DNAJA1 knockout HUVECs group, WT HUVECs with heat stress group, DNAJA1 knockout HUVECs with heat stress group,were conducted label free-based quantitative proteomic analysis to detect the mechanism of DNAJA1's protective effects on heat stress.

2024-03-26 | PXD043512 | Pride

Nse4-FLAG CHIP-seq analysis in gcn5, ubp8, ada2 deletion background in S.pombe, control wt 501 - no tag

Project description:to find functional relevance between SMC5/6 complex and SAGA histone acetyltransferase complex in Schizosaccharomyces pombe by chromatin immunoprecipitation of Nse4-FLAG tagged protein from gcn5, ubp8 and ada2 deletion strains and 2 control strains (WT - Nse4-FLAG, 501 - no tag).

2023-02-20 | E-MTAB-12401 | biostudies-arrayexpress

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