Ontology highlight
ABSTRACT:
PROVIDER: PRJNA639597 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| SRR12018971_1.fastq.gz | Fastqsanger.gz | |||
| SRR12018971_2.fastq.gz | Fastqsanger.gz | |||
| SRR12018972_1.fastq.gz | Fastqsanger.gz | |||
| SRR12018972_2.fastq.gz | Fastqsanger.gz | |||
| SRR12018973_1.fastq.gz | Fastqsanger.gz |
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Project description:Plastics are highly stable materials with widespread applications, but their resistance to degradation poses a significant environmental challenge, often resulting in accumulation in landfills or pollution in the form of microplastics. Biodegradation using insect larvae has recently emerged as a promising strategy to address this issue, though the molecular basis of plastic degradation in these organisms remains poorly understood due to limited genomic resources. In this study, we present a complete genome of the lesser wax moth, Achroia grisella, and tissue-specific RNA-Seq data of both the lesser and the greater wax moth, Galleria mellonella, two species known to consume various plastics. Our analyses reveal several highly expressed secretory enzymes in gut and labial tissues. Orthologous comparisons of differentially expressed genes also identified five enzymes (three hexamerins and two monooxygenases) from the lesser wax moth that have been shown or are predicted to have plastic-degrading potential in the greater wax moth. We also identified enzymes that may potentially be involved in polyethylene and polystyrene degradation based on their identities with known bacterial enzymes that have been experimentally validated and are involved in plastic degradation pathways. Together, these genomic and transcriptomic resources provide a foundation for understanding plastic degradation in wax moths and highlight candidate genes for future functional validation.
2026-03-08 | GSE322731 | GEO
Project description:Plastics are highly stable materials with widespread applications, but their resistance to degradation poses a significant environmental challenge, often resulting in accumulation in landfills or pollution in the form of microplastics. Biodegradation using insect larvae has recently emerged as a promising strategy to address this issue, though the molecular basis of plastic degradation in these organisms remains poorly understood due to limited genomic resources. In this study, we present a complete genome of the lesser wax moth, Achroia grisella, and tissue-specific RNA-Seq data of both the lesser and the greater wax moth, Galleria mellonella, two species known to consume various plastics. Our analyses reveal several highly expressed secretory enzymes in gut and labial tissues. Orthologous comparisons of differentially expressed genes also identified five enzymes (three hexamerins and two monooxygenases) from the lesser wax moth that have been shown or are predicted to have plastic-degrading potential in the greater wax moth. We also identified enzymes that may potentially be involved in polyethylene and polystyrene degradation based on their identities with known bacterial enzymes that have been experimentally validated and are involved in plastic degradation pathways. Together, these genomic and transcriptomic resources provide a foundation for understanding plastic degradation in wax moths and highlight candidate genes for future functional validation.
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Project description:Dietary oligosaccharides are prebiotics that fuel gut microbes, but individual microbiomes may respond differently depending on oligosaccharide structure and microbiome composition and function. The extent to which specific gut microbial communities exhibit personalized functional responses to distinct oligosaccharides remains underexplored. We applied a standardized ex vivo microbiome culture, called RapidAIM, coupled with metaproteomics to examine how six structurally diverse oligosaccharides affect the gut microbiota functional response.
2026-01-13 | PXD064733 | Pride
Project description:Polyethylene pollutions are considered inert in nature and adversely affect the entire ecosystem. Larvae of greater wax moths (Galleria mellonella) have the ability to masticate and potentially biodegrade polyethylene films at elevated rates. The wax moth has been thought to metabolize PE independently of gut flora, however, the role of the microbiome is poorly understood and degradation by the wax moth might be involved. To determine whether the salivary glands of the wax moth were potentially involved in the PE degradation, it was investigated how surface changes of polyethylene were affected by mastication and consumption. Formation of pitting and degradation intermediates, including carbonyl groups, indicated that salivary glands could assist in polyethylene metabolism. We investigated the biochemical effect of exposure to PE on the composition of the salivary gland proteome. The expression of salivary proteins was found to be affected by PE exposure. The proteins that were significantly affected by the exposure to PE revealed that the wax moth is undergoing general changes in energy levels, and that enzymatic pathways associated with fatty acid beta oxidation were induced during PE consumption.
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Project description:Galleria mellonella larvae efficiently biodegrade LDPE plastic via gut monooxygenase activity.
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Project description:Effect of certain microorganisms on mouse gut microbial communities in aged group.
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Project description:Gut microbes elicit specific changes in gene expression in the colon of mice. We colonized germ-free mice with microbial communities from the guts of humans, zebrafish and termites, human skin and tongue, soil and estuarine microbial mats. We used microarrays to detail the differences in global gene expression in colon tissue that are caused by the different microbial communities 28 days after gavage into the germfree animal. Three biological replicates per group, male C57BL/6 mice (12-16 weeks old)
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Project description:The study aimed to explore the potential of bacterial biodegradation as a solution to the global problem of plastic pollution, specifically targeting polyethylene (PE), one of the most common types of plastic. The goals of the study were to isolate a bacterial strain capable of breaking down PE, identify the key enzymes responsible for the degradation process, and understand the metabolic pathways involved. By investigating these aspects, researchers sought to gain critical insights that could be used to optimize plastic degradation conditions and inform the development of artificial microbial communities for effective bioremediation strategies. This research has significant relevance, as it addresses the pressing need for innovative and sustainable approaches to tackle the ever-growing issue of plastic waste and its impact on the environment.
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