Project description:Cajal-Retzius cells (CR cells) are the earliest born neurons in the cerebral cortex, and have been implicated in neuronal migration and development of cortical circuitry. One of the sources of CR cells is the cortical hem, which is rich in morphogens of the canonical WNT signaling family. In this study we examine the effect of stabilization of β-CATENIN (gain of function, GOF) in the mouse cortical hem and show that although neurons are still produced, they do not acquire a CR cell identity. The trajectory of differentiation examined using single-cell transcriptomics revealed that upon β-CATENIN GOF, hem-derived CR cells do not display a TBR2+ stage characteristic of immature CR cells. This effect is only seen when β-CATENIN GOF is driven in hem progenitors and is not seen upon β-CATENIN GOF in postmitotic CR cells. These data suggest that a TBR2+ stage may be important for hem-derived CR cell development and that this step appears to be sensitive to levels of stabilized β-CATENIN in hem progenitors.
Project description:Characterization of three hepatocyte cell lines (2 parental and 1 clone), under two different media conditions. Parental cell lines HepG2 and HC-04, single cell cloned from HC-04 defined as HC-04.J7. The goal of the project initially was to investigate the invasion of hepatocytes by Plasmodium falciparum sporozoites, and once found that the HC-04 cell had a higher invasion than HepG2, single cell isolates were generated from HC-04 before being expanded. The resulting HC-04.J7 isolate further improved the invasion rate. Transcriptomic and proteomic data sets were generated from all cell lines in both media, than analyzed for potential receptors or biochemical pathways that play a role in the increased invasion of HC-04 and specifically, HC-04.J7.
Project description:Metagenome data from soil samples were collected at 0 to 10cm deep from 2 avocado orchards in Channybearup, Western Australia, in 2024. Amplicon sequence variant (ASV) tables were constructed based on the DADA2 pipeline with default parameters.
Project description:In an abundant aquatic groups of microorganisms, SAR11, the transition between salt- and freshwater environments has happened only once: all freshwater SAR11 belong to subclade IIIb/LD12, which has also been found to inhabit coastal environments where salinity varies widely. The first reported isolates of the SAR11 freshwater clade LD12 and a member of the sister clade IIIa from the same region are now available. This project quantifed concentrations of select, known intracellular metabolites within two strains of SAR11: LSUCC0261 and LSUCC0530.