Plant root sample
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA640497 | ENA |
REPOSITORIES: ENA
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Project description:Inter-plant communication is key for the successful thriving of plant communities in nature with yet untapped potential for agroecosystem engineering towards more resilient crops and plant defences against pathogens. Arbuscular mycorrhizal fungi (AMF) are ubiquitous root symbionts, well-known to constitute underground common mycelial networks (CMNs) linking multiple plant hosts underground. The wired connection among plants via CMNs are hypothesized to play a key role for information exchange between plants for the neighbour-primed inter-plant defences. However, the key transcriptomic and metabolome responses in receiver plant associated with inter-plant CMN connections remain yet to be elucidated. Moreover, no studies to date have clearly resolved the confounding effects of hyphal damage from the inter-plant network disconnection. To uncover the contribution of CMN integrity to neighbour-primed plant defences, we used model AMF Rhizophagus irregularis to inter-connect two Medicago truncatula plants and explored the effect of sender wounding and flg22 elicitation on receiver plants’ leaf responses and pathogen tolerance. For the first time, we demonstrate that changes in receiver plants’ biotic stress and defence signalling pathways rely on inter-plant signals via the CMN, not on mycelial network damage itself. This response was associated with distinct leaf isoprenoid production, including volatile monoterpenes and triterpene saponins. Furthermore, CMN-mediated signals from stressed senders enhanced receiver resistance to Fusarium sporotrichoides whilst simultaneously increasing susceptibility to Botrytis cinerea. Our findings highlight the critical role of CMNs in inter-plant signalling for pathogen-specific susceptibility and resistance which can be a key for understanding plant community-level defence in nature and agroecosystems.
2026-06-06 | GSE325469 | GEO
Project description:Root exudates play major roles in the recruitment of plant microbiota. The metabolic composition of root exudates varies according to plant developmental stage, nutrient availability, (a)biotic stresses and interaction with the root-associated microbiota, including arbuscular mycorrhizal fungi (AMF), which play a key role in plant mineral nutrition and stress tolerance. While it is well established that AMF can perceive plant root exudate compounds, little is known about plant root exudate modifications in response to AMF inoculation. Here, we developed an aeroponic-based culture system suitable for the analysis of maize root exudates during symbiosis with the AMF Rhizophagus irregularis while controlling nutrient availability. We validated the functionality of the system by monitoring both maize root colonization by the AMF and the expression profile of symbiotic root marker genes. We then investigated the composition of root exudates (strigolactones and specialized metabolites) from mycorrhizal and non-mycorrhizal plants grown under different N and P regimes. Comparisons of specialized metabolite profiles from root exudates, root tissues, and fungal extracts allowed us to identify candidate metabolic features specifically accumulating in mycorrhizal root exudates. Thus, we provide an innovative method to better understand the role of root exudate metabolites in shaping the microbiota of mycorrhizal plants.
2025-05-22 | MSV000097969 | MassIVE
Project description:Anthropogenic nutrient inputs alter soil biodiversity; however, it remains largely unknown whether changes in soil microeukaryotes (fungi and protists) are primarily driven by direct effects, such as modifications in soil properties, or by indirect effects, such as plant diversity loss. To disentangle these mechanisms, we investigated the long-term effects (11 years) of fertilization and manipulated plant diversity (1, 2, or 4 plant species) on soil microeukaryote communities in a temperate grassland experiment using long-amplicon rRNA sequencing. Our results indicate that fertilization generally had a stronger influence on microeukaryote communities than plant species richness. Fertilization altered the community composition of fungi and protists, increased OTU richness by 20.8% and 52.7%, respectively, and shifted community dominance from fungi to protists. Regarding plant diversity, we observed an effect exclusively on the protist community. Changes were primarily explained by increased plant biomass (driven by both fertilization and plant diversity) and by higher soil phosphorus and lower soil pH levels (driven exclusively by fertilization). Regarding life strategies, we observed synergistic treatment effects: fertilization primarily enhanced fungal saprophytes (only richness), fungal animal pathogens, and protist consumers, whereas plant diversity affected phototrophic protists (reduction) and protist animal pathogens (enhancement). Notably, fertilization and plant diversity decline together led to a cumulative increase in fungal plant pathogens. In conclusion, we highlight that fertilisation alone has a significant effect on soil microeukaryotes, while the additional decline in plant diversity affects different soil groups that are not directly affected by fertilisation. This synergistic pattern indicates that fertilization can influence the entire microeukaryote community through direct and indirect mechanisms, with a cumulative enhancement on certain groups, such as plant pathogens.
2026-07-06 | E-MTAB-15101 | biostudies-arrayexpress
Project description:Fungal community in plant root Raw sequence reads
| PRJNA687162 | ENA
Project description:Many of the microorganisms that are normally present in the soil, actually inhabit the rhizosphere and interact with plants. Those plant–microorganisms interactions may be beneficial or harmful. Among the first are the arbuscular mycorrhizal fungi (AMF). These soil fungi have been reported to improve plant resistance/tolerance to pests and diseases. On the other hand, soilborne pathogens represent a threat to agriculture generating important yield losses, depending upon the pathogen and the crop. One example is the “Sudden Death Syndrome” (SDS), a severe disease in soybean (Glycine max (L.) Merr) caused by a complex of at least four species of Fusarium sp., among which Fusarium virguliforme and F. tuccumaniae are the most prevalent in Argentina. This study provides, under strict in vitro culture conditions, a global analysis of transcript modifications in mycorrhizal and non-mycorrhizal soybean root associated with F. virguliforme inoculation. Microarray results showed qualitative and quantitative changes in the expression of defense-related genes in mycorrhizal soybean, suggesting that AMF are good candidates for sustainable plant protection against F. virguliforme.
2018-11-12 | GSE108964 | GEO
Project description:AMF community diversity in ecological floating beds
| PRJNA699679 | ENA