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Arabidopsis RBV is a conserved WD40 repeat protein that promotes microRNA biogenesis and ARGONAUTE1 loading

ABSTRACT: Arabidopsis RBV is a conserved WD40 repeat protein that promotes microRNA biogenesis and ARGONAUTE1 loading

PROVIDER: PRJNA640818 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR12060854.fastq.gz	Fastqsanger.gz
	SRR12060855.fastq.gz	Fastqsanger.gz
	SRR12060856.fastq.gz	Fastqsanger.gz
	SRR12060857.fastq.gz	Fastqsanger.gz
	SRR12060858.fastq.gz	Fastqsanger.gz

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Arabidopsis RBV is a conserved WD40 repeat protein that promotes microRNA biogenesis and ARGONAUTE1 loading[RNA-seq]

Project description:Arabidopsis RBV is a conserved WD40 repeat protein that promotes microRNA biogenesis and ARGONAUTE1 loading[RNA-seq]

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Arabidopsis RBV is a conserved WD40 repeat protein that promotes microRNA biogenesis and ARGONAUTE1 loading [sRNA-seq]

Project description:Arabidopsis RBV is a conserved WD40 repeat protein that promotes microRNA biogenesis and ARGONAUTE1 loading [sRNA-seq]

| PRJNA640823 | ENA

Arabidopsis RBV is a conserved WD40 repeat protein that promotes microRNA biogenesis and ARGONAUTE1 loading[RNA-seq]

Project description:MicroRNAs (miRNAs) play crucial roles in gene expression regulation through RNA cleavage or translation repression. Here, we report the identification of an evolutionarily conserved WD40 domain protein as a player in miRNA biogenesis in Arabidopsis thaliana. A mutation in the REDUCTION IN BLEACHED VEIN AREA (RBV) gene encoding a WD40 domain protein led to the suppression of leaf bleaching caused by an artificial miRNA; the mutation also led to a global reduction in the accumulation of endogenous miRNAs. The nuclear protein RBV promotes the transcription of MIR genes into pri-miRNAs by enhancing the occupancy of RNA polymerase II (Pol II) at MIR gene promoters. RBV also promotes the loading of miRNAs into AGO1. In addition, RNA-seq revealed a global splicing defect in the mutant. Thus, this evolutionarily conserved, nuclear WD40 domain protein acts in miRNA biogenesis and RNA splicing.

2022-02-26 | GSE152910 | GEO

Arabidopsis RBV is a conserved WD40 repeat protein that promotes microRNA biogenesis and ARGONAUTE1 loading [sRNA-seq]

2022-02-26 | GSE152909 | GEO

Arabidopsis RBV is a conserved WD40 repeat protein that promotes microRNA biogenesis and ARGONAUTE1 loading.

Project description: Not available

| S-EPMC8904849 | biostudies-literature

Arabidopsis RBV is a conserved WD40 repeat protein that promotes microRNA biogenesis and ARGONAUTE1 loading

Project description:This SuperSeries is composed of the SubSeries listed below.

2022-02-26 | GSE152911 | GEO

stem transcriptome in a triple wd40 mutant.-Gene disruption in a triple WD40 mutant.

Project description:ra16-04_3wd40 - stem transcriptome in a triple wd40 mutant. - Are cell wall genes affected in a triple WD40 mutant (LWD1xLWD2xTTG1)? - Not specified

2018-09-17 | GSE119889 | GEO

stem transcriptome in a triple wd40 mutant.-Gene disruption in a triple WD40 mutant.

Project description:stem transcriptome in a triple wd40 mutant.-Gene disruption in a triple WD40 mutant.

| PRJNA490641 | ENA

Systematic functional profiling of WD40 proteins reveals Wcp1, a cyclophilin that links CO2/heat tolerance to acidic pH adaptation in Cryptococcus neoformans [RNA-Seq]

Project description:WD40 domains are major protein–protein interaction (PPI) scaffolds, yet their contributions to fungal pathogenicity remain poorly defined. We systematically analysed 94 canonical WD40 proteins in Cryptococcus neoformans. Conditional expression and sporulation analyses identified 36 essential WD40 proteins, while in vitro and in vivo profiling of 103 signature-tagged deletion strains spanning 52 genes uncovered 31 pathogenicity-related WD40 proteins, including epigenetic and post-transcriptional regulators. We highlighted Wcp1, a WD40–cyclophilin fusion required for growth at 37 °C under 5% CO₂. Both modules supported CO₂/heat tolerance and virulence. Notably, Wcp1 couples these functions to acidic pH adaptation: wcp1Δ mutants failed to grow under elevated temperature and CO₂ at acidic pH, exhibited enhanced intracellular acidification, reduced survival within macrophages and attenuated virulence in both Drosophila and murine models. Integrated transcriptomic and proteomic analyses revealed that Wcp1 acts as a central integrator of intracellular pH homeostasis by coordinating proton transport, metabolic adaptation and stress-buffering networks.

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Hierarchical rules for Argonaute loading in Drosophila

Project description:Drosophila Argonaute-1 and Argonaute-2 differ in function and small RNA content. AGO2 binds to siRNAs, whereas AGO1 is almost exclusively occupied by microRNAs. microRNA duplexes are intrinsically asymmetric, with one strand, the miR strand, preferentially entering AGO1 to recognize and regulate the expression of target mRNAs. The other strand, miR*, has been viewed as a by-product of microRNA biogenesis. Here, we show that miR*s are often loaded as functional species into AGO2. This indicates that each microRNA precursor can potentially produce two mature small RNA strands that are differentially sorted within the RNAi pathway. miR* biogenesis depends upon the canonical microRNA pathway, but loading into AGO2 is mediated by factors traditionally dedicated to siRNAs. By inferring and validating hierarchical rules that predict differential AGO loading, we find that intrinsic determinants, including structural and thermodynamic properties of the processed duplex, regulate the fate of each RNA strand within the RNAi pathway. These were independently processed and sequenced using the Illumina GAII platform. In total, five libraries were analyzed.

2009-11-15 | E-GEOD-17734 | biostudies-arrayexpress

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