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RNA-seq analysis of mammary tissue reveals persistent changes in gene expression profiles dependent upon the level of folic acid intake in C56BL/6 mice.

ABSTRACT: RNA-seq analysis of mammary tissue reveals persistent changes in gene expression profiles dependent upon the level of folic acid intake in C56BL/6 mice.

PROVIDER: PRJNA643823 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR12137000_1.fastq.gz	Fastqsanger.gz
	SRR12137000_2.fastq.gz	Fastqsanger.gz
	SRR12137001_1.fastq.gz	Fastqsanger.gz
	SRR12137001_2.fastq.gz	Fastqsanger.gz
	SRR12137002_1.fastq.gz	Fastqsanger.gz

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Project description:Four-times daily milking during the first 3 weeks of lactation elicits an increase in milk yield, which persists through late lactation even after twice-daily milking is imposed. We hypothesized that this milk yield response would be associated with changes in mammary proliferation, apoptosis, and gene expression, which would persist throughout lactation. Six multiparous cows were assigned to unilateral frequent milking (UFM; twice daily milking of the left udder half (2X), four-times daily milking of the right udder half (4X)) on days 1 to 21 of lactation, followed by 2X thereafter. Udder halves initially milked 4X produced more milk than those milked 2X during, and after UFM treatment, through 180 days in milk (DIM). To determine the mechanisms involved in the persistent milk yield response, we obtained mammary biopsies from both udder halves at 21, 23, and 40 DIM. Rates of [3H]-thymidine incorporation into DNA in vitro and mammary cell apoptosis were not affected by UFM or DIM. Using Affymetrix GeneChipM-BM-. Bovine Genome Arrays, we determined that the differential expression (4X vs. 2X) of 18 genes was significantly affected by DIM. Within the group of 18 differentially expressed genes, we identified a cluster of 15 genes with a similar temporal pattern of differential expression. Nine of the genes in the cluster remained differentially expressed at 40 DIM, indicating that they may be involved in the persistent milk yield response. Among the genes in the cluster were chitinase 3-like (CHI3L)-1, clusterin, early growth response (EGR)-1, and sex determining region Y-box (SOX)-4. These genes have been associated with mammary development, differentiation and remodeling; all of which may be functionally related to the increase in milk yield. We conclude that frequent milking during early lactation does not alter mammary growth but is associated with changes in mammary expression of 18 genes. Future experiments will determine the function of these genes in the mammary gland, and will clarify their role in the autocrine regulation of milk production and long-term alteration of mammary function. 36 samples from 6 cows; 3 timepoints

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RNA-seq analysis of mammary tissue reveals persistent changes in gene expression profiles dependent upon the level of folic acid intake in C56BL/6 mice.

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