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RNA-seq analysis of mammary tissue reveals persistent changes in gene expression profiles dependent upon the level of folic acid intake in C56BL/6 mice.

ABSTRACT: RNA-seq analysis of mammary tissue reveals persistent changes in gene expression profiles dependent upon the level of folic acid intake in C56BL/6 mice.

PROVIDER: PRJNA643823 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR12137000_1.fastq.gz	Fastqsanger.gz
	SRR12137000_2.fastq.gz	Fastqsanger.gz
	SRR12137001_1.fastq.gz	Fastqsanger.gz
	SRR12137001_2.fastq.gz	Fastqsanger.gz
	SRR12137002_1.fastq.gz	Fastqsanger.gz

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RNA-seq analysis of mammary tissue reveals persistent changes in gene expression profiles dependent upon the level of folic acid intake in C56BL/6 mice.

Project description:Purpose: To identify, using RNA-sequencing, differentially expressed genes in the mammary gland of mice supplemented with FA. We aimed to ascertain whether gene expression is persistently altered after increased FA supplementation, whether such alterations are related to genes implicated in epigenetic regulation and disease risk. Methods: Mammary gland mRNA profiles of mice 4 weeks after folic acid supplementation (5mg/kg vs 1mg/kg) during adulthood were generated by deep sequencing using RNA sequencing. The sequence reads that passed quality filters were aligned to the mouse genome using TopHat and analyzed at the gene level using DESeq2. qRT–PCR validation was performed using SYBR Green assays Results: Using an optimized data analysis workflow, we mapped reads to the mouse genome (build mm10) and identified 222 genes differentially expressed between the two group (adjsuted p value <0.05). 191 of these were upregulated and 31 were downregulated. Geneset enrichment analysis identified several enriched pathways, including epithelial mesenchymal transition and myogenesis. Conclusions: Our study provides novel insights into the persistent effects of FA intake on the C57BL/6 mammary transcriptome and identifies putative gene targets and molecular pathways that help to understand how increasing FA intake may modulate the long term expression of gene networks and disease pathways.

2020-09-15 | GSE153732 | GEO

Folic acid intake during juvenile/peri-pubertal development modulates the mammary tissue transcriptome of female C56BL/6 mice

Project description:Folic acid intake during juvenile/peri-pubertal development modulates the mammary tissue transcriptome of female C56BL/6 mice

| PRJNA789906 | ENA

Folic acid intake during juvenile/peri-pubertal development modulates the mammary tissue transcriptome of female C56BL/6 mice

Project description:We tested the hypothesis that high FA (HFA) intake alters the juvenile/peri-pubertal mammary transcriptome in a manner consistent with increased potential for breast cancer, detectable beyond the period of intake. C57BL/6 mice were fed control FA (CFA) (1 mg/kg diet) or HFA (5 mg/kg diet) diets for 4 weeks, followed by AIN93M maintenance diet for 4 weeks. RNA sequencing of the mammary transcriptome (~83 million reads) showed 18 transcripts (17 downregulated) differentially expressed between groups. Gene Set Enrichment showed downregulated genes significantly enriched in oxidative phosphorylation, adipogenesis, fatty acid metabolism and epithelial mesenchymal transition. Cancer and developmental disorder were the most altered Disease and Disorder pathways, with top upstream regulators identified as DNA methyltransferases. This study demonstrates HFA intake during juvenile/peri-pubertal mammary development induces changes in the mammary transcriptome associated with altered energy metabolism, cell neoplasia and epigenetic regulation of gene.

2024-12-01 | GSE191160 | GEO

A folic acid-enriched diet attenuates prostate involution in response to androgen deprivation

Project description:Folic acid is present in pre-natal vitamins, fortified cereal grains and multi-vitamin supplements. High intake of folic acid through these sources has resulted in populations with increased levels of serum folate and unmetabolized folic acid. Although the benefits of folic acid in the prevention of neural tube defects are undeniable, the impact of long-term consumption of folic acid on the prostate is not fully understood. In this study, we used a rodent model to test whether dietary folic acid (FA) supplementation changes prostate homeostasis and response to androgen deprivation. Although intact prostate weights do not differ between diet groups, we made the surprising observation that dietary folic acid supplementation confers partial resistance to castration-mediated prostate involution. More specifically, male mice that were fed a folic acid supplemented diet and then castrated had greater prostate wet weights, greater prostatic luminal epithelial cell heights, and more abundant RNAs encoding prostate secretory proteins compared to mice that were fed a control diet and castrated. We used RNA-seq to identify signaling pathways enriched in the castrated prostates from folic acid supplemented diet fed mice compared to control mice. We observed differential expression of genes involved in several metabolic pathways in the FA supplemented mice. Together, our results show that dietary FA supplementation can impact metabolism in the prostate and attenuate the prostate’s response to androgen deprivation. This has important implications for androgen deprivation therapies used in the treatment of prostate disease, as consumption of high levels of folic acid could reduce the efficacy of these treatments.

2018-08-22 | GSE116299 | GEO

Maternal folic acid overconsumption in mice leads to sex-specific transcriptional alterations in cerebral cortex in offspring

Project description:Recent concerns have been raised regarding high folate intake during pregnancy and lactation, particularly following food fortiﬁcation and increased vitamin supplement use. Epidemiologic studies have shown that high folic acid (FA) intake can negatively impact motor and neurocognitive development in offspring. We used microarray to investigate the mechanisms by which a supplemented diet containing 5-fold higher FA than recommended (FASD) affects brain function in both male and female offspring.

2024-03-03 | GSE134588 | GEO

Nanopore sequencing reveals the roles of spermatozoal DNA N6-Methyladenine modification in mediating transgenerational lipid metabolism disorder induced by folic acid excessive consumption in chicken [RNA-seq]

Project description:Increased consumption of folic acid is prevalent due to its beneficial effects to help protecting from genome damage. Growing evidence is emphasizing the side effects pointing to excessive dietary intake. Effects of paternal folic acid excessive consumption on the offspring and its transgenerational inheritance mechanism remain elusive. We hypothesize that the folic acid excessive consumption will alter sperm DNA N6-methyladenine (6mA) and 5-methylcytosine (5mC) methylation, and heritably influence offspring metabolic homeostasis. This is the results of mRNA sequencing part

2020-12-16 | GSE163238 | GEO

folic acid

Project description:Caco-2, HT29, CCD841CoTr exposed to 10ng/mL, 100ng/mL and 4000ng/mL folic acid

2010-06-10 | E-GEOD-2047 | biostudies-arrayexpress

Mus musculus strain:C56BL/6J

Project description:Mus musculus strain:C56BL/6J Raw sequence reads

| PRJNA1112412 | ENA

Maternal folic acid over consumption in mice leads to sex-specific transcriptional alterations in cerebral cortex in offspring

Project description:Recent concerns have been raised regarding high folate intake during pregnancy and lactation, particularly following food fortiﬁcation and increased vitamin supplement use. Epidemiologic studies have shown that high folic acid (FA) intake can negatively impact motor and neurocognitive development in offspring. We used microarray to investigate the impact of a supplemented diet containing 5-fold higher FA than recommended (5xFASD) on cerebral transcription profile in both male and female postnatal day (P) 30 pups. We also studied gene expression changes due to sex.

2022-03-15 | GSE193355 | GEO

Testicular MTHFR deficiency may explain sperm DNA hypomethylation associated with high dose folic acid supplementation

Project description:Supplementation with high doses of folic acid, an important mediator of one-carbon transfers for DNA methylation, is used clinically to improve sperm parameters in infertile men. We recently detected an unexpected loss of DNA methylation in the sperm of idiopathic infertile men after 6 months of daily supplementation with 5mg folic acid (>10× the daily recommended intake-DRI), exacerbated in men homozygous for a common variant in the gene encoding an important enzyme in folate metabolism, methylenetetrahydrofolate reductase (MTHFR 677C>T). To investigate the epigenomic impact and mechanism underlying effects of folic acid on male germ cells, wildtype and heterozygote mice for a targeted inactivation of the Mthfr gene were fed high-dose folic acid (10× the DRI) or control diets for six months. No changes were detected in general health, sperm counts or methylation of imprinted genes. Reduced representation bisulfite sequencing revealed sperm DNA hypomethylation in Mthfr+/- mice on the 10× diets. Wildtype mice demonstrated sperm hypomethylation only with a very high dose (20×) of folic acid for 12 months. Testicular MTHFR protein levels decreased significantly in wildtype mice on the 20× diet but not in those on the 10× diet, suggesting a possible role for MTHFR deficiency in sperm DNA hypomethylation. In-depth analysis of the folic acid-exposed sperm DNA methylome suggested mouse/human susceptibility of sequences with potential importance to germ cell and embryo development. Our data provide evidence for a similar cross-species response to high dose folic acid supplementation, of sperm DNA hypomethylation, and implicate MTHFR downregulation as a possible mechanism.

2018-01-23 | GSE100849 | GEO

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