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ABSTRACT: RNA-seq of IPEC-J2 cell

PROVIDER: PRJNA644872 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR12172855_1.fastq.gz	Fastqsanger.gz
	SRR12172855_2.fastq.gz	Fastqsanger.gz
	SRR12172856_1.fastq.gz	Fastqsanger.gz
	SRR12172856_2.fastq.gz	Fastqsanger.gz
	SRR12172857_1.fastq.gz	Fastqsanger.gz

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Comparing two intestinal porcine epithelial cell lines (IPECs): global expression patterns to characterise a in vitro model of intestinal physiology

Project description:The intestinal epithelial cell lines 1 and J2 (IPEC-1, IPEC-J2) - spontaneously immortalised cell lines from the porcine intestine - are important tools for studying intestinal function. Microarrays (GeneChip Porcine Genome Array) were used to compare the expression pattern at basal in vitro conditions. Expression analyses complemented by morphological, functional and biochemical analyses revealed that IPEC-J2 is a morphologically and functionally more differentiated cell line in comparison to IPEC-1. In addition, IPEC-2 cells are a preferential tool for in vitro studies with the focus on metabolism.

2015-12-31 | GSE67407 | GEO

Transcriptome-wide m6A methylome of intestinal porcine epithelial cells (IPEC-J2) induced by deoxynivalenol

Project description:Deoxynivalenol (DON) frequently detected in a wide range of foods and feeds, inducing cytotoxicity to animals and humans. N6-methyladenosine (m6A) is an important epitranscriptomic marker with high abundance in eukaryotic mammals mRNAs. However, the role of the m6A methylomes in DON damage is still poorly understood. Here, we investigated the m6A transcriptome-wide profile in intestinal porcine epithelial cells (IPEC-J2) with and without 1000 ng/mL DON treatment via m6A sequencing and RNA sequencing. In total, 5406 new m6A peaks appeared with the disappearance of 2615 peaks in DON-induced IPEC-J2. The unique m6A-modified genes in DON-induced IPEC-J2 were associated with TNF signaling pathway. We identified 733 differentially expressed mRNA transcripts with hyper-methylated or hypo-methylated m6A peaks between DON-induced IPEC-J2 and normal IPEC-J2. Protein interaction network analysis and qPCR validation suggested that CSF2 probably acts as a promising new target for combating DON damage in IPEC-J2. Our first report of m6A transcriptome-wide map of IPEC-J2 cells presented here provides a starting roadmap for uncovering m6A functions that may affect DON infection.

2020-08-13 | GSE156078 | GEO

Project description:RNA-seq of IPEC-J2 cells

| PRJNA862330 | ENA

Sus scrofa

Project description:NLRC5 Knockdown IPEC-J2 cell and NLRC5 normal IPEC-J2 cell Transcriptome

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Project description:RNA-sequencing of IPEC-J2

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Project description:IPEC-J2 cell transcriptome sequencing

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Effect of antimicrobial peptide PR-39 on the gene expression of porcine intestinal epithelial cell line (IPEC-J2)

Project description:To gain a more complete understanding of how porcine cathelicidin PR-39 influence the porcine intestinal epithelial cells, we profiled gene expression patterns in IPEC-J2 cell line in the presence or the absence of PR-39.

2014-06-28 | GSE58876 | GEO

Project description:RNA-seq of PEDV Infection in IPEC-J2 Cells

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Amplicon sequencing data of sgRNA cassettes from pig CRISPR screening time course to identify essential genes in PK15 and IPEC-J2 cell lines

Project description:This study describes a genome-wide CRISPR-Cas9 knockout (GeCKO) screen performed in two porcine cell lines, PK15 and IPEC-J2. A custom-designed porcine GeCKO (pGeCKO) library targeting protein-coding genes, lncRNAs, and miRNAs was used. Genomic DNA was harvested from GeCKO cell populations at four timepoints between 3-26 days post-transduction in each cell line. Amplicon sequencing libraries were generated from guide RNA regions using custom primers with Illumina adapter sequences, and sequenced on the NovaSeq 6000 platform (PE150). This dataset enables quantification of guide RNA abundance over time and identification of essential genes in pig cells, supporting research in comparative genomics, functional genomics, and translational animal models.

2025-08-30 | E-MTAB-15487 | biostudies-arrayexpress

RNA-seq of IPEC-J2 cells samples from different miRNA treatment groups

Project description:RNA-seq of IPEC-J2 cells samples from different groups to search for miRNA effect on gene expression. Lentivirus of miRNA 133, and miRNA 143 were used to knock down (KD), or overexpress (OV) the two miRNAs. LV3NC is the control for knock down, Lv20NC is control for overexpression. LV133 is the miRNA 133 knockdown, LV143 is the miRNA 133 knock down. OV143 is the miRNA 143 overexpression. miRNA 133 is no overexpression.

2019-10-31 | GSE138428 | GEO

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