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Ignition of supporting cell activation for hair cell regeneration in the avian auditory epithelium: an explant culture model

ABSTRACT: Ignition of supporting cell activation for hair cell regeneration in the avian auditory epithelium: an explant culture model

PROVIDER: PRJNA646062 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR12214485_1.fastq.gz	Fastqsanger.gz
	SRR12214485_2.fastq.gz	Fastqsanger.gz
	SRR12214486_1.fastq.gz	Fastqsanger.gz
	SRR12214486_2.fastq.gz	Fastqsanger.gz
	SRR12214487_1.fastq.gz	Fastqsanger.gz

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Similar Datasets

Ignition of supporting cell activation for hair cell regeneration in the avian auditory epithelium: an explant culture model

Project description:Sensorineural hearing loss is included in the most common disabilities, and often caused by loss of sensory hair cells in the cochlea. Hair cell regeneration has long been a main target for developing novel therapeutics for sensorineural hearing loss. In the mammalian cochlea, hair cell regeneration occurs in very limited situations, while auditory epithelia of non-mammalians retain the capacity for hair cell regeneration. In the avian basilar papilla, an auditory sensory epithelium, supporting cells, which are sources for regenerated hair cells, are usually quiescent, while hair cell loss induces both direct transdifferentiation of supporting cells and mitotic division of supporting cells. In the present study, we aimed to establish an explant culture model for hair cell regeneration in chick basilar papillae, and validated usefulness of our model to investigate the initial phase of hair cell regeneration. Histological assessments demonstrated that hair cell regeneration via direct transdifferentiation of supporting cells occurred in our model. Labeling assay using 5-ethynyl-2'-deoxyuridine (EdU) revealed the occurrence of mitotic division of supporting cells in the specific location in basilar papillae, while no EdU labeling was identified in newly generated HCs. RNA sequencing indicated alterations in known signaling pathways associated with hair cell regeneration, which is consistent with previous findings. In addition, unbiased analyses of RNA sequencing data indicated novel genes and signaling pathways that could be related to the ignition of supporting cell activation in chick basilar papillae. These results indicate the advantages of our model using explant cultures of chick basilar papillae for exploring molecular mechanisms for hair cell regeneration. Further studies such as single-cell RNA sequencing will allow us to capture the spatiotemporal information by using our explant culture model.

2020-10-23 | GSE154375 | GEO

High-resolution transcriptional analysis reveals stepwise fate conversion of supporting cells to hair cells in the chicken auditory epithelium

Project description:In contrast to mammals, the avian cochlea, specifically the basilar papilla, can regenerate sensory hair cells, which involves fate conversion of supporting cells to hair cells. To determine the mechanisms for converting supporting cells to hair cells, we used single-cell RNA sequencing during hair cell regeneration in explant cultures of chick basilar papillae. We identified dynamic changes in the gene expression of supporting cells, and the pseudotime trajectory analysis demonstrated the stepwise fate conversion from supporting cells to hair cells. Initially, supporting cell identity was erased and transition to the precursor state occurred. A subsequent gain in hair cell identity progressed together with downregulation of precursor-state genes. Transforming growth factor beta receptor 1-mediated signaling was involved in induction of the initial step, and its inhibition resulted in suppression of hair cell regeneration. Our data provide new insights for understanding fate conversion from supporting cells to hair cells in avian basilar papillae.

2023-01-10 | GSE209791 | GEO

EDNRB2 regulates fate determination, migration and maturation of hair-cell precursors in regenerating avian auditory epithelia

Project description:The mammalian cochlea loses the ability for hair cell (HC) regeneration after birth, while in the avian auditory epithelium, namely the basilar papilla (BP), supporting cells (SCs) retain the capability for HC regeneration throughout life. Our previous study using single-cell RNA sequencing indicated stepwise fate conversion of SCs to HCs via the precursor state, in which EDNRB2 exhibited specifically high expression, in the process of HC regeneration in chick BP. The present study aimed to reveal a distinct role of EDNRB2 in HC regeneration in chick BP. During HC regeneration in chick BP explant cultures, EDNRB2 expression emerged in a part of ATOH1-expressing SCs immediately after HC loss and decreased according to the progress of HC regeneration process. In embryonic chick BP, EDNRB2 expression was specifically identified in the precursor cell state, which confirmed that mature SCs were reprogrammed to the precursor state in response to HC loss. Pharmacological inhibition of endothelin receptor type B (EDNRB) signaling decreased regenerated HCs in chick BP explant cultures. RNA sequencing revealed that inhibition of EDNRB signaling downregulated genes for HC differentiation and maturation together with genes for cell migration. Histological assessments clarified the deterioration of migration of HC precursors and the delay of HC regeneration processes by inhibition of EDNRB signaling. These results indicate that the expression of EDNRB2 contributes to the fate determination of HCs and that EDNRB signaling regulates the migration and maturation of HC precursors during chick HC regeneration.

2025-06-21 | GSE287538 | GEO

Mapping the regulatory landscape of auditory hair cells from single cell multi-omics data

Project description:Auditory hair cells transduce sound to the brain and in mammals these cells reside together with supporting cells in the sensory epithelium of the cochlea, called the organ of Corti. To establish the organ’s delicate function during development and differentiation, spatiotemporal gene expression is strictly controlled by chromatin accessibility and cell type specific transcription factors, jointly representing the regulatory landscape. Bulk-sequencing technology and cellular heterogeneity obscured investigations on the interplay between transcription factors and chromatin accessibility in inner ear development. To study the formation of the regulatory landscape in hair cells, we collected single-cell chromatin accessibility profiles accompanied by single-cell RNA data from genetically labeled murine hair cells and supporting cells after birth.

2021-03-11 | GSE157398 | GEO

Cell-type identity of the avian utricle

Project description:The avian utricle, a vestibular organ of the inner ear, displays turnover of sensory hair cells throughout life. This is in sharp contrast to the mammalian utricle, which shows limited regenerative capacity. Here, we use single-cell RNA-sequencing to identify distinct marker genes for the different sensory hair cell subtypes of the chicken utricle, which we validated in situ . We provide markers for spatially distinct supporting cell populations, and identified two transitional cell populations of dedifferentiating supporting cells and developing hair cells. Trajectory reconstruction resulted in an inventory of gene expression dynamics of natural hair cell generation in the avian utricle.

2022-09-07 | GSE212831 | GEO

Gene Expression from hair cells and supporting cells in chick utricle

Project description:In adult mammals, hair cell loss is irreversible and may result in hearing and balance deficits. In contrast, birds can regenerate hair cells through differentiation of supporting cells and restore inner ear function, suggesting that hair cell progenitors are present in the population of supporting cells. We used microarrays to identify novel genes related to the regeneration in the chicken utricle. Supporting cell and hair cell populations of chicken utricle obtained by laser capture microdissection, following to do RNA extraction and hybridization on Affymetrix microarrays.

2016-05-17 | E-GEOD-81461 | biostudies-arrayexpress

Initiation of Supporting Cell Activation for Hair Cell Regeneration in the Avian Auditory Epithelium: An Explant Culture Model.

Project description:Sensorineural hearing loss is a common disability often caused by the loss of sensory hair cells in the cochlea. Hair cell (HCs) regeneration has long been the main target for the development of novel therapeutics for sensorineural hearing loss. In the mammalian cochlea, hair cell regeneration is limited, but the auditory epithelia of non-mammalian organisms retain the capacity for hair cell regeneration. In the avian basilar papilla (BP), supporting cells (SCs), which give rise to regenerated hair cells, are usually quiescent. Hair cell loss induces both direct transdifferentiation and mitotic division of supporting cells. Here, we established an explant culture model for hair cell regeneration in chick basilar papillae and validated it for investigating the initial phase of hair cell regeneration. The histological assessment demonstrated hair cell regeneration via direct transdifferentiation of supporting cells. Labeling with 5-ethynyl-2'-deoxyuridine (EdU) revealed the occurrence of mitotic division in the supporting cells at specific locations in the basilar papillae, while no EdU labeling was observed in newly generated hair cells. RNA sequencing indicated alterations in known signaling pathways associated with hair cell regeneration, consistent with previous findings. Also, unbiased analyses of RNA sequencing data revealed novel genes and signaling pathways that may be related to the induction of supporting cell activation in the chick basilar papillae. These results indicate the advantages of our explant culture model of the chick basilar papillae for exploring the molecular mechanisms of hair cell regeneration.

| S-EPMC7688741 | biostudies-literature

EDNRB2 regulates fate determination, migration and maturation of hair-cell precursors in regenerating avian auditory epithelia

Project description:EDNRB2 regulates fate determination, migration and maturation of hair-cell precursors in regenerating avian auditory epithelia

| PRJNA1213701 | ENA

Gene Expression from hair cells and supporting cells in chick utricle

2016-05-17 | GSE81461 | GEO

High-resolution transcriptional analysis reveals stepwise fate conversion of supporting cells to hair cells in the chicken auditory epithelium

Project description:High-resolution transcriptional analysis reveals stepwise fate conversion of supporting cells to hair cells in the chicken auditory epithelium

| PRJNA862478 | ENA

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