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Gene expression in gamma-glutamylcyclotransferase-overexpressing mouse embryonic fibroblast NIH3T3 cells.

ABSTRACT: Gene expression in gamma-glutamylcyclotransferase-overexpressing mouse embryonic fibroblast NIH3T3 cells.

PROVIDER: PRJNA646743 | ENA |

REPOSITORIES: ENA

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Gene expression in gamma-glutamylcyclotransferase-overexpressing mouse embryonic fibroblast NIH3T3 cells.

Project description:Although hypoxia inducible factor-1a (HIF-1a) was originally identified as a transcriptional factor to adapt hypoxia, HIF-1a has also been shown to regulate metabolic pathways specific for cancer, including aerobic glycolysis, namely “Warburg effect”. However, the mechanisms by which HIF-1a mediates the metabolic pathway under normoxia are not fully understood. Here, we identified gamma-glutamylcyclotransferase (GGCT) as a novel regulator of HIF-1a. GGCT is a highly expressed protein in various cancer tissues. Knockdown of GGCT exerts anticancer effects both in vitro and in vivo; thus, it is considered a promising therapeutic target. In this study, we show that depleting GGCT downregulates the protein and mRNA expressions of HIF-1a in PC3 prostate cancer cells, and that overexpressing GGCT upregulates them in mouse fibroblast NIH3T3 cells. We also show that depleting GGCT downregulates HIF-1a downstream target genes involved in glycolysis, whereas overexpressing GGCT exhibits upregulation pattern in these genes. Furthermore, we suggest that GGCT induces the metabolic switch from citric acid cycle to glycolysis under normoxia. Additionally, we show that GGCT regulates expression of HIF-1a protein via AMPK-mTORC1-4EBP1 pathway in PC3 cells. These results indicate the GGCT plays a regulatory role in the expression of HIF-1a followed by glycolytic switch in cancer cells.

2020-07-17 | GSE154592 | GEO

Mus musculus strain:mouse embryonic fibroblast (MEF) cells line NIH3T3

Project description:Mus musculus strain:mouse embryonic fibroblast (MEF) cells line NIH3T3 Raw sequence reads

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Mus musculus strain:mouse embryonic fibroblast (MEF) cells line NIH3T3

Project description:Mus musculus strain:mouse embryonic fibroblast (MEF) cells line NIH3T3 Raw sequence reads

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Role of SIRT7 overexpression in mouse fibroblast cells NIH3T3

Project description:Human SIRT7 GFP or GFP alone were overexpressed in NIH3T3 cells.In confluent conditions, SIRT7GFP overexpressing cells but not GFP expressing cells showed presence of distinct compact cellular colonies composed of small sized cells (ST7). Along with this SIRT7GFP overexpressing cells with long and fibroblast morphoogy(LT7) were also present. Single cell clones from these cells were expanded to form ST7 (Small SIRT7GFP expressing) and LT7 (Long SIRT7GFP expressing) lines. RNA from GFP expressing cells (SKGFP), ST7 and LT7 lines was then isolated and applied to microarray analysis to identify gene expression changes in two cell lines

2020-07-14 | GSE148486 | GEO

Expression profile of NIH3T3 cells overexpressing FOXP2 or a FOXP2 arrangement (FOXP2-CPED1)

Project description:To study the underlying mechanism of tumor initation driven by FOXP2, we performed transcriptome analysis of transformed NIH3T3 overexpressing FOXP2 or FOXP2-CEPD1 and control cells to explore the FOXP2-related transcriptome in tumorigenesis.

2019-05-08 | GSE114350 | GEO

Role of SIRT7 overexpression in mouse fibroblast cells NIH3T3

Project description:Role of SIRT7 overexpression in mouse fibroblast cells NIH3T3

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Expression data from transgenic mice overexpressing RXR-gamma in the skeletal muscle (RXR-gamma mice)

Project description:Retinoid X receptor (RXR)-gamma is a nuclear receptor-type transcription factor expressed mostly in the skeletal muscle, and regulated by nutritional conditions. Previously, we established transgenic mice overexpressing RXR-gamma in the skeletal muscle (RXR-gamma mice), which showed lower blood glucose than the control mice. We used microarrays to investigate their glucose metabolism gene expression change.

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Identification of Protein Expression Changes During Fibroblast Activation in Response to LAMP2A Overexpression

Project description:Proteomic analysis comparing LAMP2A-TGFβtreated group versus Vector-TGFβ 3 treated group in NIH3T3 fibroblast cells to study the effect of LAMP2A overexpression on protein expression changes During Fibroblast Activation.

2024-09-02 | PXD055463 |

Gene expression changes associted with overexpression of Foxc1 transcription factor in NIH3T3 fibroblasts

Project description:To identify pathways transcriptionally controlled by Foxc1, NIH3T3 fibroblasts stably overexpressing Foxc1 ORF, or an empty retroviral vector control, were subjected to RNA-sequencing.

2025-06-27 | GSE297719 | GEO

Effects of Nogo-B receptor overexpression on transcriptomic profiling in the NIH3T3 fibroblast cell line

Project description:Unrestrained receptor tyrosine kinase (RTK) signaling and epigenetic dysregulation are responsible in part for tumorigenesis. Aberrant RTK signaling results in the remodeling of histone modification. As a Ras modulator, NgBR promotes Ras-mediated RTK signaling pathways. Meanwhile, we observed that NgBR overexpression in NIH3T3 cells significantly increased the acetylation of histone H3 and histone H4. To further elucidate the underlying mechanism that NgBR upregulates histone acetylation in tumorigenesis, we carried out RNA-seq analysis to examine the transcriptome alternation in NIH3T3 cells and NgBR-overexpressed NIH3T3 cells (3T3NgBR cells) and revealed that overexpression of NgBR up-regulates ACSS2 expression, but not ACSS1 and ACSS3. Moreover. ACSS2 overexpression abrogated NgBR deficiency-caused decrease of histone acetylation in NIH3T3 cells. Taken together, all the data suggested that NgBR regulates tumorigenesis in NIH3T3 cells via ACSS2-mediated histone acetylation.

2025-05-01 | GSE253418 | GEO

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