Plant metagenome
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA646829 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| SRR12352497_1.fastq.gz | Fastqsanger.gz | |||
| SRR12352497_2.fastq.gz | Fastqsanger.gz |
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Project description:<p>Plant-leaf endophytic fungi mutualism is essential for plants to adapt to adverse environments. Though introgression has been extensively studied in many plants, the underlying effects of introgression on fungi-driven adaptability still remain unanswered. Here, we performed landscape-scale sampling of natural populations across its typical distribution range in China to disentangle how introgression effects endophytic fungal community within oak leaves. Our results showed that fungal diversity was decreased while fungal co-occurrence network complexity was increased with increasing introgression index, reflecting a “dual adaptation strategy” to respond to environmental challenges. This pattern was also linked to metacommunity: the lowest diversity occurred in metacommunity that characterized by the lowest mean annual temperature and annual precipitate, whereas the highest fungal network complexity appeared in metacommunity that had the highest mean annual temperature and optimal annual precipitate. Importantly, the differential metabolite intensities (e.g., organoheterocyclic compounds, and organoheterocyclic compounds) and climate factors mediated the leaf endophytic fungi-driven adaptability of oak trees. This work not only advances our understanding of plant-microbe interactions in the context of evolutionary ecology but also provides insight into the importance of fungal community restructuring in facilitating plant adaptation to environmental change.</p>
2025-11-28 | MTBLS13410 | MetaboLights
Project description:Transgenic expression of a double-stranded RNA in plants can induce silencing of homologous mRNAs in fungal pathogens. Although such host-induced gene silencing is well documented, the molecular mechanisms by which RNAs can move from the cytoplasm of plant cells across the plasma membrane of both the host cell and fungal cell are poorly understood. Indirect evidence suggests that this RNA transfer may occur at a very early stage of the infection process, prior to breach of the host cell wall, suggesting that silencing RNAs might be secreted onto leaf surfaces. To assess whether Arabidopsis plants possess a mechanism for secreting RNA onto leaf surfaces, we developed a protocol for isolating leaf surface RNA separately from intercellular (apoplastic) RNA. This protocol yielded abundant leaf surface RNA that displayed an RNA banding pattern distinct from apoplastic RNA, suggesting that it may be secreted directly onto the leaf surface rather than exuded through stomata or hydathodes. Notably, this RNA was not associated with either extracellular vesicles or protein complexes; however, RNA species longer than 100 nucleotides could be pelleted by ultracentrifugation. Furthermore, pelleting was inhibited by the divalent cation chelator EGTA, suggesting that these RNAs may form condensates on the leaf surface. These leaf surface RNAs are derived almost exclusively from Arabidopsis, but come from diverse genomic sources, including rRNA, tRNA, mRNA, intergenic RNA, microRNAs, and small interfering RNAs, with tRNAs especially enriched. We speculate that endogenous leaf surface RNA plays an important role in the assembly of distinct microbial communities on leaf surfaces.
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Project description:<p>Entomopathogenic fungi have the ability to both directly kill insect pests and act as plant endophytic fungi to impact plant growth and development. Despite this, the widespread endophytic use of these fungi in tea plants (Camellia sinensis) is still limited. This study examined how Beauveria bassiana colonizes tea plant tissues and its impact on tea plant growth and development. Through amplicon sequencing and liquid chromatography-tandem mass spectrometry (LC-MS/MS), the study investigated the response patterns of dominant endophytic microbial populations in tea plants during fungal colonization and the alterations in secondary metabolites in tea plants due to the colonization process. B. bassiana effectively colonized tea seedlings through root irrigation and foliar spraying methods, showing a preference for stems, and the colonization persisted for more than 90 days. Colonization resulted in a reduction in the diversity and structural stability of the endophytic microbial community in tea plants; however, it also enhanced the importance of ecologically mutualistic relationships or cooperative interactions in community assembly. Additionally, colonization had a more pronounced effect on endophytic fungi compared to endophytic bacteria. It led to an increase in the relative abundance of arbuscular mycorrhizal fungi in root tissues and a decrease in the relative abundance of total plant pathogens in tea plant tissues. Terpenoids were the most significantly associated differential metabolites following B. bassiana colonization, followed by steroids and their derivatives, and flavonoids. Colonization by B. bassiana resulted in elevated levels of the majority of differential metabolites in tea plant stems at the onset of colonization. The colonization of B. bassiana in tea plants displayed a strong positive correlation with 26 distinct metabolites, such as proanthocyanidin B2 and L-malic acid. This colonization altered the endophytic microbial community, impacting metabolic pathways associated with plant hormone synthesis, volatile compound production, as well as the growth, development, and defense of tea plants.</p>
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Project description:<p>The induced capacity of plant growth-defense trade-offs under biotic stress is increasingly recognized. While many studies mainly focus on plants’ defense activation stage during insect attacks, limited attention has been given to post-stress recovery. Herein, leaves from normal and post-herbivory tea plants were collected during the compensation stage, and their microbiomes, metabolomes, and transcriptomes were used to dissect the mechanisms underlying growth-defense trade-offs. The results revealed that apart from altering the microbial community diversity, insect herbivory significantly enriched leaf-associated pathogens, particularly Alternaria spp. Network analysis and in-situ separation jointly revealed the role of Sphingomonas aquatilis in resisting pathogen invasion. Meanwhile, the restructured microbiota exhibited stronger network stability, indicating the enhancement of pathogen resistance among the endophytic community. Moreover, we found that downregulated flavonoids were defense compounds against pathogens and upregulated saccharides were plant growth-promoting compounds, which were verified in subsequent tests. Overall, tea plants compensate for reduced defense through restructuring of the endophytic microbiota, and prioritize growth over defense through metabolic resetting after insect herbivory. This study also revealed new biocontrol and growth-promoting resources for tea plants.</p>
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