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Combinatorial action of temporally-segregated transcription factors

ABSTRACT: Combinatorial action of temporally-segregated transcription factors

PROVIDER: PRJNA649565 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR12345938.fastq.gz	Fastqsanger.gz
	SRR12345939.fastq.gz	Fastqsanger.gz
	SRR12345940.fastq.gz	Fastqsanger.gz
	SRR12345941.fastq.gz	Fastqsanger.gz
	SRR12345942.fastq.gz	Fastqsanger.gz

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Combinatorial action of temporally-segregated transcription factors

Project description:Combinatorial action of transcription factors(TFs) with partially-overlapping expression is a widespreadstrategyto generate novel gene-expression patternsand thuscellular diversity. Known mechanisms underlying combinatorialactivityrequireco-expression of TFswithin the same cell. Here, we describe themechanismby which twoTFsthat are never co-expressed, generate a new, intersectional expression pattern in C. elegans embryos:lineage-specific priming of a gene by a transiently-expressedTFgeneratesa uniqueintersectionwith a second TF acting on the same gene four cell divisions later;the second TF is expressed in multiple cells butonly activatestranscription in those where priming occurred.Early induction of active transcription is necessary and sufficient to establish a competent state that is maintained by broadly-expressed regulators in absence of theinitial trigger. We further uncover new cells diversified through this mechanism. Our findings define anovel mechanism forcombinatorial TF activity with important implications for generation of cell-type diversity in vivo and in vitro.

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Combinatorial Action of Temporally Segregated Transcription Factors.

Project description:Combinatorial action of transcription factors (TFs) with partially overlapping expression is a widespread strategy to generate novel gene-expression patterns and, thus, cellular diversity. Known mechanisms underlying combinatorial activity require co-expression of TFs within the same cell. Here, we describe the mechanism by which two TFs that are never co-expressed generate a new, intersectional expression pattern in C. elegans embryos: lineage-specific priming of a gene by a transiently expressed TF generates a unique intersection with a second TF acting on the same gene four cell divisions later; the second TF is expressed in multiple cells but only activates transcription in those where priming occurred. Early induction of active transcription is necessary and sufficient to establish a competent state, maintained by broadly expressed regulators in the absence of the initial trigger. We uncover additional cells diversified through this mechanism. Our findings define a mechanism for combinatorial TF activity with important implications for generation of cell-type diversity.

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Project description:During T cell development, multipotent progenitors relinquish competence for other fates and commit to the T cell lineage by turning on Bcl11b, which encodes a transcription factor. To clarify lineage commitment mechanisms, we followed developing T cells at the single-cell level using Bcl11b knock-in fluorescent reporter mice. Notch signaling and Notch activated transcription factors collaborate to activate Bcl11b expression irrespectively of Notch-dependent proliferation. These inputs work via three distinct, asynchronous mechanisms: an early locus âpoisingâ function dependent on TCF-1 and GATA-3, a stochastic-permissivity function dependent on Notch signaling, and a separate amplitude-control function dependent on Runx1, a factor already present in multipotent progenitors. Despite their necessity for Bcl11b activation, these inputs act in a stage specific manner, providing a multitiered mechanism for developmental gene regulation. Two sets of samples were generated from DN T-cell sub-populations derived from culture of bone marrow progenitors from mice containing a knock-in Bcl11b-YFP reporter

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