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Effect of platelets on ovarian cancer cell spheroids

ABSTRACT: Effect of platelets on ovarian cancer cell spheroids

PROVIDER: PRJNA650234 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR12366638_1.fastq.gz	Fastqsanger.gz
	SRR12366638_2.fastq.gz	Fastqsanger.gz
	SRR12366639_1.fastq.gz	Fastqsanger.gz
	SRR12366639_2.fastq.gz	Fastqsanger.gz
	SRR12366640_1.fastq.gz	Fastqsanger.gz

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Effect of platelets on ovarian cancer cell spheroids

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Project description:Spheroids are 3D multi-cell aggregates formed in non-addherent culture conditions. In ovarian cancer (OC), they serve as a vehicle for cancer cell dissemination in the peritoneal cavity. We investigated genes and networks upregulated in three dimensional (3D) versus two-dimensional (2D) culture conditions by Affymetrix gene expression profiling and identified ALDH1A1, a cancer stem cell marker as being upregulated in OC spheroids. Network analysis confirmed ALDH1A1 upregulation in spheroids in direct connection with elements of the beta-catenin pathway. A parallel increase in the expression levels of beta-catenin and ALDH1A1 was demonstrated in spheroids vs. monolayers an in successive spheroid generations by using OC cell liness and primary OC cells. The percentage of Aldefluor positive cells was significantly higher in spheroids vs. monolayers in IGROV1, A2780, SKOV3, and primary OC cells. B-catenin knock-down decreased ALDH1A1 expression and chromatin immunoprecipitation demonstrated that beta-catenin directly binds to the ALDH1A1 promoter. Both siRNA mediated beta-catenin knock-down and a novel ALDH1A1 small molecule enzymatic inhibitor described here for the first time, decreased the number of OC spheroids (p<0.001) and cell viability. These data strongly support the role of beta-catenin regulated ALDH1A1 in the maintenance of OC spheroids and of a stem cell phenotype and propose new ALDH1A1 inhibitors targeting this cell population. Different gene profiles were observed in ovarian cancer spheroids versus ovarian cancer monolayers. Nine samples were analyzed in triplicate. Each group is a reference.

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Project description:To discover the core gene expression features of ovarian cancer spheroids The compared the whole transcript expression profiling between 5 pairs of spheroids and monolayer cancer cells, including the RMG1, MCAS and Ov432 plus two cases of serous ovarian cancer ascites cells.

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