Project description:Chimonocalamus is the only bamboo species known to secrete aromatic oils. The species is named after the distinctive aromatic oil found within the cavity of its bamboo stem. This study employed sensory analysis, headspace-gas chromatography-mass spectrometry (HS-SPME-GC-MS), and transcriptome sequencing to investigate the volatile component composition and formation mechanisms in Chimonocalamus delicatus, an endemic bamboo species of Yunnan Province. The bamboo samples analyzed included stems at different stem ages, as well as various parts. The aroma components of Chimonocalamus delicatus primarily consist of sesquiterpenes, including Humulene and Caryophyllene, which are terpenoid compounds. Principal component analysis (PCA) revealed significant differences in the volatile compounds across various stem ages and plant parts. This may constitute a key source of the characteristic aroma of Chimonocalamus delicatus at different stem ages and plant parts. Transcriptome analysis revealed that the aroma of Chimonocalamus delicatus was predominantly enriched in the secondary metabolite pathways, including the MVA and MEP pathways. The enzyme activities of key enzymes in its metabolic pathway were also determined. A comprehensive analysis of differential aroma metabolites and differentially expressed genes (DEGs) in Chimonocalamus delicatus led to the identification of key genes (DXR, FPS, TPS6, TPS7) involved in the regulation of aroma compound biosynthesis. In this study, the volatile components and regulatory network of Chimonocalamus delicatus essential oil were analyzed, which provided a solid theoretical basis for the development of bioactive compounds in Chimonocalamus delicatus.
Project description:Investigation of whole genome gene expression level changes in Pichia stipitis CBS 6054 grown aerobically in xylose, compared to the same strain grown aerobically in glucose.
Project description:To reveal the role of sulfur metabolism genes in memory formation processes, transcriptome libraries were obtained from the heads of 5-day-old naive males. The libraries were generated from Drosophila strains created in our laboratory with deleted cbs genes ( CBS-/-(5) and CBS-/-(8), cse (CSE-/-) and strains with double deletion of cbs and cse genes (CBS-/-,CSE-/-(1) and (CBS-/-,CSE-/-(2). Strain 58492, in which deletions were introduced by the CRISP/CAS9 method, was used as a control strain.
Project description:Investigation of whole genome gene expression level changes in Pichia stipitis CBS 6054 grown aerobically in xylose, compared to the same strain grown aerobically in glucose. A six array study using total RNA recovered from three separate cultures of Pichia stipitis CBS 6054 grown in glucose and three separate cultures of Pichia stipitis CBS 6054 grown in xylose. Each array measures the expression level of 374,100 probes (average probe length 53.6 +/- 4.1 nt) tiled across the Pichia stipitis CBS 6054 genome with a median spacing distance of 33 nt. During data processing, probes are filtered to include only those probes corresponding to annotated protein-coding genes.