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Time-course transcriptome analysis of non-antibiotics producer and actinorhodin over-producing Streptomyces coelicolor strain

ABSTRACT: Time-course transcriptome analysis of non-antibiotics producer and actinorhodin over-producing Streptomyces coelicolor strain

PROVIDER: PRJNA655669 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR12400263.fastq.gz	Fastqsanger.gz
	SRR12400264.fastq.gz	Fastqsanger.gz
	SRR12400265.fastq.gz	Fastqsanger.gz
	SRR12400266.fastq.gz	Fastqsanger.gz
	SRR12400267.fastq.gz	Fastqsanger.gz

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Time-course transcriptome analysis of non-antibiotics producer and actinorhodin over-producing Streptomyces coelicolor strain

Project description:To identify unique gene expression in higher antibiotics producing Streptomyces coelicolor strain, non-producer M1146 and the derivative strain M1146+ACT (M1146 with actinorhodin biosynthetic genes cluster) was choosen for comparative transcriptome analysis. The genes with different gene expression might be key genes important for antibiotics production.

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Project description:This SuperSeries is composed of the following subset Series: GSE22394: S. coelicolor M145 SMM medium time course study (Culture # 1) GSE22395: S. coelicolor M145 SMM medium time course study (Culture # 2) GSE22397: S. coelicolor M145 SMM medium time course study (Culture # 3) Refer to individual Series

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S. coelicolor YSK4425 Time-course Study in R5- medium (Culture#2)

Project description:Antibiotic biosynthesis in Streptomyces species is controlled by a complex genetic and biochemical network of global and pathway specific regulators. Details of their precise interactions in mediating temporal and spatial expression of secondary metabolite genes remain poorly defined. In this study, we employed whole-genome microarrays to investigate the temporal transcriptome profiles of S. coelicolor A3(2) afsS::apr mutant strain (YSK4425) and compare it to wild-type M145 strain. The regulatory protein encoded by afsS is known to affect antibiotic biosynthesis (Floriano, B., Bibb, M. 1996. afsR is a pleiotropic but conditionally required regulatory gene for antibiotic production in Streptomyces coelicolor A3(2). Mol Microbiol, 21, 385-96). Keywords: Time course

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Response of Streptomyces coelicolor to treatment with the antibiotics vancomycin bacitracin and moenomycin A

Project description:To determine the response at the transcriptional level of S. coelicolor cells to treatment with 3 antibiotics that target distinct stages of cell wall biosynthesis, biological triplicate cultures of strain M600 were treated with sub-lethal concentrations (10 µg/ml) of vancomycin, bacitracin or moenomycin A. Samples were taken 0, 30, 60, 90 minutes after addition of the antibiotic. A negative control which received no drug treatment was also performed.

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Project description:Antibiotic biosynthesis in Streptomyces species is controlled by a complex genetic and biochemical network of global and pathway specific regulators. Details of their precise interactions in mediating temporal and spatial expression of secondary metabolite genes remain poorly defined. In this study, we employed whole-genome microarray methods to investigate the temporal transcriptome profiles of S. coelicolor A3(2) ∆afsS::apr mutant strain and compared with the M145 Wild-type strain (series submitted separately). Keywords: Time course

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Project description:Antibiotic biosynthesis in Streptomyces species is controlled by a complex genetic and biochemical network of global and pathway specific regulators. Details of their precise interactions in mediating temporal and spatial expression of secondary metabolite genes remain poorly defined. In this study, we employed whole-genome microarray methods to investigate the temporal transcriptome profiles of S. coelicolor A3(2) ∆absA1::apr mutant strain and compared with the M145 Wild-type strain (series submitted separately). Keywords: Time course

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Project description:Antibiotic biosynthesis in Streptomyces species is controlled by a complex genetic and biochemical network of global and pathway specific regulators. Details of their precise interactions in mediating temporal and spatial expression of secondary metabolite genes remain poorly defined. In this study, we employed whole-genome microarray methods to investigate the temporal transcriptome profiles of S. coelicolor A3(2) ∆absA1::apr mutant strain and compared with the M145 Wild-type strain (series submitted separately). Keywords: Time course

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Project description:Antibiotic biosynthesis in Streptomyces species is controlled by a complex genetic and biochemical network of global and pathway specific regulators. Details of their precise interactions in mediating temporal and spatial expression of secondary metabolite genes remain poorly defined. In this study, we employed whole-genome microarrays to investigate the temporal transcriptome profiles of S. coelicolor A3(2) M145 wild type and disruption mutants of regulatory genes (afsS and absA1) known to affect antibiotic biosynthesis. Keywords: Time course

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Project description:Antibiotic biosynthesis in Streptomyces species is controlled by a complex genetic and biochemical network of global and pathway specific regulators. Details of their precise interactions in mediating temporal and spatial expression of secondary metabolite genes remain poorly defined. In this study, we employed whole-genome microarrays to investigate the temporal transcriptome profiles of S. coelicolor A3(2) M145 wild type and disruption mutants of regulatory genes (afsS and absA1) known to affect antibiotic biosynthesis. Keywords: Time course

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