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The microRNA landscape of MYCN-amplified neuroblastoma

ABSTRACT: The microRNA landscape of MYCN-amplified neuroblastoma

PROVIDER: PRJNA656107 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR12417900.fastq.gz	Fastqsanger.gz
	SRR12417901.fastq.gz	Fastqsanger.gz
	SRR12417902.fastq.gz	Fastqsanger.gz
	SRR12417903.fastq.gz	Fastqsanger.gz
	SRR12417904.fastq.gz	Fastqsanger.gz

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The microRNA landscape of MYCN-amplified neuroblastoma

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The Super Elongation Complex Drives Transcriptional Addiction in MYCN-amplified Neuroblastoma

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Project description:Purpose: Identify new targets in MYCN-amplified Neuroblastoma Methods: ChIP-Seq experiments were performed on Kelly and LAN-1 neuroblastoma cells by using the following antibodies: anti-EZH2 (Cell Signaling 5246S); anti-H3K27me3 (Millipore 07-449); anti-H3K4me3 (Abcam ab8580). We evaluated the global EZH2 PRC2-dependence by identifiying direct genome-wide target genes for EZH2, H3K27me3 and H3K4me3. Results: We found that EZH2 serves a PRC2-dependent function in neuroblastoma, repressing neuronal differentiation. Moreover, EZH2-regulated genes were strongly repressed in MYCN-amplified and high-risk primary tumors. Conclusion: Our study supports testing EZH2 inhibitors in patients with MYCN-amplified neuroblastoma.

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