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Pervasive promoter-proximal regulation in archaea: RNA-seq of cells during stationary phase

ABSTRACT: Pervasive promoter-proximal regulation in archaea: RNA-seq of cells during stationary phase

PROVIDER: PRJNA657270 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR12455257_1.fastq.gz	Fastqsanger.gz
	SRR12455257_2.fastq.gz	Fastqsanger.gz
	SRR12455258_1.fastq.gz	Fastqsanger.gz
	SRR12455258_2.fastq.gz	Fastqsanger.gz
	SRR12455259_1.fastq.gz	Fastqsanger.gz

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Pervasive promoter-proximal regulation in archaea: ChIP-seq stationary phase

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Pervasive promoter-proximal regulation in archaea: ChIP-seq stationary phase

Project description:Recruitment of RNA polymerase and initiation factors to the promoter is the only known mechanisms for transcription activation and repression in archaea. Whether any of the subsequent steps towards productive transcription elongation is involved in regulation is not known. We characterised how the basal transcription machinery is distributed along genes in the archaeon Sulfolobus solfataricus. We discovered a distinct early elongation phase where RNA polymerases sequentially recruit the elongation factors Spt4/5 and Elf1 to form the transcription elongation complex (TEC) before the TEC escapes into productive transcription. TEC escape is rate-limiting for transcription output during exponential growth. Oxidative stress causes changes in TEC escape that correlate with changes in the transcriptome. Our results thus establish that TEC escape contributes to the basal promoter strength and facilitates transcription regulation. Impaired TEC escape coincides with the accumulation of initiation factors at the promoter and recruitment of termination factor aCPSF1 to the early TEC. This suggests two possible mechanisms for how TEC escape limits transcription, physically blocking upstream RNA polymerases during transcription initiation and premature termination of early TECs.

2020-09-14 | GSE141617 | GEO

Pervasive promoter-proximal regulation in archaea: RNA-seq of cells during stationary phase

Project description:Recruitment of RNA polymerase and initiation factors to the promoter is the only known mechanisms for transcription activation and repression in archaea. Whether any of the subsequent steps towards productive transcription elongation is involved in regulation is not known. We characterized how the basal transcription machinery is distributed along genes in the archaeon Sulfolobus solfataricus. We discovered a distinct early elongation phase where RNA polymerases sequentially recruit the elongation factors Spt4/5 and Elf1 to form the transcription elongation complex (TEC) before the TEC escapes into productive transcription. TEC escape is rate-limiting for transcription output during exponential growth. Oxidative stress causes changes in TEC escape that correlate with changes in the transcriptome. Our results thus establish that TEC escape contributes to the basal promoter strength and facilitates transcription regulation. Impaired TEC escape coincides with the accumulation of initiation factors at the promoter and recruitment of termination factor aCPSF1 to the early TEC. This suggests two possible mechanisms for how TEC escape limits transcription, physically blocking upstream RNA polymerases during transcription initiation and premature termination of early TECs.

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Pervasive promoter-proximal regulation in archaea: ChIP-exo exponential growth

Project description:Pervasive promoter-proximal regulation in archaea: ChIP-exo exponential growth

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Pervasive promoter-proximal regulation in archaea: Permanganate ChIP-seq exponential growth

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Project description:Pervasive promoter-proximal regulation in archaea: ChIP-exo hydrogen peroxide-treated cells

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