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RNA-Seq analysis of wild-type and ttpA-C240ins mutant D. discoideum amebae in surface and liquid growth

ABSTRACT: RNA-Seq analysis of wild-type and ttpA-C240ins mutant D. discoideum amebae in surface and liquid growth

PROVIDER: PRJNA659064 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR12511654_1.fastq.gz	Fastqsanger.gz
	SRR12511654_2.fastq.gz	Fastqsanger.gz
	SRR12511655_1.fastq.gz	Fastqsanger.gz
	SRR12511655_2.fastq.gz	Fastqsanger.gz
	SRR12511656_1.fastq.gz	Fastqsanger.gz

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RNA-Seq analysis of wild-type and ttpA-C240ins mutant D. discoideum amebae in surface and liquid growth

Project description:In many eukaryotes, mRNAs containing specific AU-rich motifs are regulated by proteins of the tristetraprolin (TTP) family, which bind to these motifs through a tandem zinc finger (TZF) domain. This binding leads to promotion of subsequent deadenylation and decay, partly through a conserved carboxyl-terminal CNOT1 binding domain. We explored the physiological role of the single TTP family member (TtpA) expressed in Dictyostelium discoideum. This study shows the effect of an insertional mutation within the TZF domain (ttpA-C240ins), which creates a null genotype, evaluated in amebae in growth medium during surface culture and liquid culture under axenic conditions. The mutant cells exhibited severe growth defects and a multinucleate phenotype during liquid culture, but not in surface culture.

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Project description:In many eukaryotes, mRNAs containing specific AU-rich motifs are regulated by proteins of the tristetraprolin (TTP) family, which bind to these motifs through a tandem zinc finger (TZF) domain. This binding leads to promotion of subsequent deadenylation and decay, partly through a conserved carboxyl-terminal CNOT1 binding domain. We explored the physiological role of the single TTP family member (TtpA) expressed in Dictyostelium discoideum. This study shows the effect of an insertional mutation in the TZF domain (ttpA-C240ins), which disrupted the TZF RNA binding domain, evaluated in amebae in axenic conditions in growth medium during surface culture, and during short term (2 and 6 hours) nutrient deprivation. RNA-Seq analysis was performed on WT and mutant cells in parallel during this short time course.

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