Project description:mRNA expression data for VPS39-silenced human muscle cells and controls (n=6 per group) were obtained using GeneChip™ Human Gene 2.0 ST Assay (Applied Biosystems)
Project description:mRNA expression data for the mouse muscle (n=6 per sex and genotype, for a total of 24 mice) were obtained using Clariom™ S Assay Mouse (Applied Biosystems) according to the manufacturer’s recommendations.
Project description:The African swine fever virus (ASFV) is a large and complex DNA virus that causes a highly le-thal disease in swine, for which no antiviral drugs or vaccines are currently available. Studying viral–host protein-protein interactions advances our understanding of the molecular mecha-nisms underlying viral replication and pathogenesis and can facilitate the discovery of antiviral therapeutics. In this study, we employed affinity tagging and purification mass spectrometry to characterize the interactome of VPS39, an important cellular factor during the early phase of ASFV replication. The interaction network of VPS39 revealed associations with mitochondrial proteins involved in membrane contact sites formation and cellular respiration. We show that ASFV proteins CP204L and A137R target VPS39 by interacting with its clathrin heavy chain func-tional domain. Furthermore, we elaborate on the potential mechanisms by which VPS39 may contribute to ASFV replication and prioritize interactions for further investigation into mito-chondrial protein function in the context of ASFV infection.
Project description:Purpose: found out the regulated genes of nulliplex-branch and its forming molecular mechanism Methods: the GhBRC1 genes of nulliplex branch and short branch cotton are silenced by VIGS, and then the shoot apical mRNA of controls and treated were sequenced, in four repeats, using Illumina HiSeq 2000. Results: we found 3519 and 17 differnent expressed genes in nulliplex-branch and short branch cotton, respectively. Conclusions: Our study represents the genes control development of lateral branch.
Project description:African swine fever virus (ASFV) is a large DNA virus causing a highly contagious disease in domestic and wild boar, for which no treatment is available. ASFV vaccine development is hindered by large gaps in knowledge considering virus protein function and virus-host interaction. This study shows that the ASFV CP204L is a multifunctional protein engaged in endosomal trafficking and localizes to virus replication sites. Moreover, VPS39, a component of the HOPS complex, is a direct host interactor of CP204L. The virus CP204L binds the VPS39 domain responsible for its recruitment to the endosomal membrane, prevents its integration into the HOPS complex, and promotes the clustering of lysosomes. Additionally, we show that VPS39 is an important factor in the early phase of infection, as virus replication and protein synthesis in VPS39 knockout cells are delayed. These results uncover a novel function of viral protein CP204L and extend our understanding of complex interaction between virus and host, providing insights for developing a vaccine to prevent and control ASFV.