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RNA Sequencing Analysis of Wild Type and Myrf +/− mice Retina

ABSTRACT: RNA Sequencing Analysis of Wild Type and Myrf +/− mice Retina

PROVIDER: PRJNA662876 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR12623327_1.fastq.gz	Fastqsanger.gz
	SRR12623327_2.fastq.gz	Fastqsanger.gz
	SRR12623328_1.fastq.gz	Fastqsanger.gz
	SRR12623328_2.fastq.gz	Fastqsanger.gz
	SRR12623329_1.fastq.gz	Fastqsanger.gz

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RNA Sequencing Analysis of Wild Type and Myrf +/− mice Retina

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Project description:Our research revealed that the transmembrane transcription factors MYRF-1 and MYRF-2 are crucial for activating the microRNA lin-4 at the end of the first larval stage in C. elegans. To determine whether MYRF regulates other microRNA genes during this period, we conducted a microRNA sequencing analysis to compare microRNA expression in late L1 larvae of wild type and myrf-1(ju1121) mutants. The myrf-1(ju1121) mutation causes a loss of function in both MYRF-1 and MYRF-2. Our analysis identified a subset of microRNAs that were differentially expressed between the wild type and the myrf-1(ju1121) mutants.

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Project description:The rod photoreceptor-specific neural retina leucine zipper protein Nrl is essential for rod differentiation and plays a critical role in regulating gene expression. In the mouse retina, rods account for 97% of the photoreceptors; however, in the absence of Nrl (Nrl-/-), no rods are present and a concomitant increase in cones is observed. Using mouse GeneChips (Affymetrix), we have generated expression profiles of the wild-type and Nrl-/- retina at three time-points representing distinct stages of photoreceptor differentiation. Experiment Overall Design: Both the Nrl-/- mice and wild type controls were of a matched mixed genetic background (R1 and C57BL/6 strains).

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