Ontology highlight
ABSTRACT:
PROVIDER: PRJNA663106 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| SRR12662100_1.fastq.gz | Fastqsanger.gz | |||
| SRR12662100_2.fastq.gz | Fastqsanger.gz | |||
| SRR12662101_1.fastq.gz | Fastqsanger.gz | |||
| SRR12662101_2.fastq.gz | Fastqsanger.gz | |||
| SRR12662102_1.fastq.gz | Fastqsanger.gz |
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Similar Datasets
Project description:Codonopsis pilosula, a kind of traditional Chinese medicine, possesses anti-aging effect. However, the anti-aging effect of Codonopsis pilosula on lung remains largely unknow and the exact molecular mechanism of Codonopsis pilosula anti-aging also needs to be further studied. Thus, we investigated the protective effect of Codonopsis pilosula on aging lung and the exact molecular mechanism of Codonopsis pilosula anti-aging. To test this hypothesis, we established the aging mice model, and then used Codonopsis pilosula to intervene. Microarray analysis and bioinformatics methods were used to comprehensively analyze lncRNA-miRNA-mRNA (ceRNA) network. Our results showed that the aging mice model was established. From the microarray analysis, 138 lncRNAs, 128 mRNAs and seven miRNAs significantly changed after aging, whereas, 282 lncRNAs, 283 mRNAs and 19 miRNAs were dysregulated after intervention of Codonopsis pilosula. KEGG pathway analysis was performed to explore the signaling pathways involved. Compared with the ceRNA network in aging mice and after intervention of Codonopsis pilosula, we found three mRNAs (Hif3a, Zbtb16, Plxna2) and one lncRNA (NONMMUT063872) associated with Codonopsis pilosula anti-aging and they were validated by qRT-PCR. Altogether, our results showed that Codonopsis pilosula has the protective effect on aging lung and the ceRNA network plays an important role in Codonopsis pilosula anti-aging.
2020-02-26 | GSE139875 | GEO
Project description:Codonopsis pilosula, a kind of traditional Chinese medicine, possesses anti-aging effect. However, the anti-aging effect of Codonopsis pilosula on lung remains largely unknow and the exact molecular mechanism of Codonopsis pilosula anti-aging also needs to be further studied. Thus, we investigated the protective effect of Codonopsis pilosula on aging lung and the exact molecular mechanism of Codonopsis pilosula anti-aging. To test this hypothesis, we established the aging mice model, and then used Codonopsis pilosula to intervene. Microarray analysis and bioinformatics methods were used to comprehensively analyze lncRNA-miRNA-mRNA (ceRNA) network. Our results showed that the aging mice model was established. From the microarray analysis, 138 lncRNAs, 128 mRNAs and seven miRNAs significantly changed after aging, whereas, 282 lncRNAs, 283 mRNAs and 19 miRNAs were dysregulated after intervention of Codonopsis pilosula. KEGG pathway analysis was performed to explore the signaling pathways involved. Compared with the ceRNA network in aging mice and after intervention of Codonopsis pilosula, we found three mRNAs (Hif3a, Zbtb16, Plxna2) and one lncRNA (NONMMUT063872) associated with Codonopsis pilosula anti-aging and they were validated by qRT-PCR. Altogether, our results showed that Codonopsis pilosula has the protective effect on aging lung and the ceRNA network plays an important role in Codonopsis pilosula anti-aging.
2020-02-26 | GSE139874 | GEO
Project description:Objective: To explore the molecular mechanism of Codonopsis pilosula in enhancing the immune function of aging mice, the expression profile of long-chain non-coding RNA (lncRNA), mRNA in spleen of mice was detected. Methods: An aging mouse model was established, the aging mice were treated with low, middle and high doses of Codonopsis pilosula, and the aging related indexes, spleen pathology and cellular ultrastructure were detected and analyzed. Then microarray analysis and bioinformatics methods were used to analyze the lncRNA and mRNA expression profile. GO enrichment analysis and KEGG pathway analysis were performed to analyze the differential genes between groups using KOBAS. Finally, the common lncRNAs and mRNAs in high-dose Codonopsis pilosula treatment group compared with the model group and the model group compared with the control group were selected for lncRNA-mRNA co-expression network construction, and real-time quantitative polymerase chain reaction (qRT-PCR) was used to verify the genes in the network. Results: The aging mouse model was successfully established. Codonopsis pilosula can improve the pathology and cellular ultrastructure of spleen in aging mice. Microarray analysis showed that 96 lncRNAs and 65 mRNAs changed significantly after aging, 623 lncRNAs and 435 mRNAs expressed abnormally after high-dose Codonopsis pilosula treatment. KEGG pathways involved in aging and high-dose Codonopsis pilosula treatment are mainly related to immunity, including allograft rejection, graft-versus-host disease, autoimmune thyroid disease, antigen processing and presentation and so on. The three mRNAs (Enpp6, Cped1, and Galnt15) in lncRNA-mRNA co-expression network may be related to Codonopsis pilosula improving the immunity of aging mice, which were verified by qRT-PCR. Conclusion: Codonopsis pilosula has a certain protective effect on the spleen of aging mice and the lncRNA-mRNA network may play an important role in enhancing the immunity of aging mice.
2022-05-17 | GSE150773 | GEO
Project description:To investigate the underlying mechanism that Codonopsis Pilosula anti-aging in gastrointestinal tract of aged mice induced by D-galactose.we integrated stomach and intestine tissues, and aimed to explore the underlying mechanism that Codonopsis Pilosula participates in protecting gastrointestinal structure and regulating gastrointestinal function by microarray and real-time quantitative polymerase chain reaction (qRT-PCR).
2020-12-31 | GSE140949 | GEO
Project description:To unravel the molecular mechanisms underpinning Codonopsis pilosula drought stress tolerance, we conducted comprehensive comparative transcriptome and physiological analyses of two C. pilosula cultivars that had been exposed to drought treatment for four days.
2024-11-20 | GSE270657 | GEO
Project description:In this study, distinctive biological CDs derived from Codonopsis pilosula were designed and synthesized. In traditional Chinese medicine, Codonopsis pilosula is considered to be a good blood tonic. The synthesized CDs comprise sp2/sp3 carbon atoms and oxygen/nitrogen-based groups and exhibit good biocompatibility and membrane permeability. The in vitro and in vivo experiments were conducted using an erythroid differentiation system and a mouse model, respectively, and showed that the CDs significantly promote the proliferation of erythroid cells. To reveal the detailed mechanisms of CDs in stimulating erythroblast proliferation, we performed RNA sequencing to analyze the gene expression profile change between untreated andmCDs treated erythroblasts.
2024-12-31 | GSE245893 | GEO
Project description:Introduction: This study investigates the active component Mannose-B from Codonopsis pilosula and its effect on human trophoblast cell function, particularly focusing on the regulation of Laminin Subunit Beta 1 (LAMB1) expression and its implications in subchorionic hematoma (SCH). Methods: Key genes involved in SCH pathology were identified through RNA sequencing and bioinformatics analysis. Network pharmacology was utilized to screen active components in Codonopsis pilosula and their critical targets. In vitro, HTR-8/Svneo cells were used to assess proliferation, migration, and invasion through CCK8, Transwell, and cell migration assays. A SCH rat model was established to evaluate changes in coagulation parameters, litter size, fetal viability, and fetal and placental weights. In vivo validation of Mannose-B's effects on LAMB1 expression and SCH pathology was performed using RT-qPCR and Western Blot. Results: Network pharmacology and molecular docking identified Mannose-B as an effective compound in Codonopsis pilosula, potentially beneficial for SCH treatment, with LAMB1 as a significant target. In vitro experiments showed that Mannose-B enhanced HTR-8/Svneo cell proliferation, migration, and invasion by reducing LAMB1 expression. In vivo experiments confirmed Mannose-B's inhibitory effect on placental LAMB1 expression and its potential in ameliorating SCH pathology. Conclusion: Mannose-B from Codonopsis pilosula inhibits LAMB1 expression, promoting human placental trophoblast cell proliferation, migration, and invasion, thereby mitigating the progression of SCH pathology.
2025-10-20 | PXD059213 | Pride
Project description:Codonopsis pilosula isolate:Codonopsis | cultivar:pilosula Genome sequencing
| PRJNA755884 | ENA